TheProteinStABIlityComboKitMD1-103
Thermalshiftassaysprovideinformationontheeffectvariousenvironmentalfactorshaveonthethermaldenaturationofaprotein.Thermaldenaturationisrelatedtoproteinstabilityandthereforecrystallizability.ThemostcommonthermalshiftassayisThermoFluor®,whichmeasuresincreasingfluorescenceinducedbySYPRO®Orangebindingtotheexposedproteinhydrophobiccore.Buttherearemanyothersdependingontherequirementsofyoursample. Ourproteinstabilityscreensarenowavailableinonepackageatadiscountofmorethan20%comparedtobuyingthemseparately(orapproximately4kitsforthepriceof3). TheDurhamScreens DevelopedbyEmilyCardewandEhmkePohlattheUniversityofDurham,thesekitsprovidegreaterdepthofanalysisoftheeffectofindividualbuffers,pHandsalts.TheDurhamscreenisdesignedtoworkwithNAMI,aGUI-basedprogramtoaidinterpretationofthermalshiftdata. TheDurhampHScreencontainsdeconvolutesbuffermoleculeandpHeffectsforaboradrangeofmolecule(28differentbuffers)andpH(4-11). TheDurhamSaltScreenusesmorethan30differentsalts,includingarangeoflanathanides.Itallowdiscriminationbetweendifferentanionsandcationsaswellasrevealingtheeffectsofionicstrength. ReadmoreaboutTheDurhamScreensin:Grøftehauge,MK,Hajizadeh,NR,Swann,MJandPohl,E.Protein-ligandinteractionsinvestigatedbythermalshiftassays(TSA)anddualpolarizationinterferometry(DPI).ActaCrystD71:36-44(2015). TheRUBICScreens InventedbyStaphaneBoivinandRobMeijersattheSamplePerparationandCharacterisationfacilityatEMBLHamburg.TheRubicsscreenconsistsoftwokits: TheRUBICBufferScreendeconstructsthebuffercomponentstoallowarationalinterpretationanddeconvolutionofpH,buffermoleculeandionicstrengtheffects. TheRUBICAdditiveScreenanalyzestheeffectonstabilityofvariouscommonadditivesincludingions,detergents,sugars,aminoacids,imidazole,cryoprotectantsandreducingagents.
TheRUBICScreensaredescribedin:BoivinS,KozakS,MeijersR.(2013)OptimizationofproteinpurificationandcharacterizationusingThermofluorscreens.ProteinExprPurif.91(2):192-206.
Reference:NewmanJ.(2004)Novelbuffersystemsformacromolecularcrystallization.ActaCrystallogrDBiolCrystallogr.60:610-2.
Storageconditions:
TheDurhampHScreenispresentedas96x0.5mLconditionsinadeep-wellblock.Theyarepreservativefreeanditisrecommendedthattheyarestoredat4°C,howevertheycanbeusedatroomtemperatureforscreenset-up.
RUBICBufferscreenisfreeofpreservatives.ShippingatRoomTemperature.Short-termStorageat4˚C.Itisrecommendedthatusersprefillplatesandstorethemat-20˚C.
ThermoFluorisaregisteredtrademarkofJohnsonandJohnson.SYPROisaregisteredtrademarkofLifeTechnologies.
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bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
A、Na2CO3HClH2SO4NaNO3
B、K2CO3H2SO4HNO3BaCl2
C、HClHNO3AgNO3NaCl
D、NaOHFeCl3MgCl2BaCl2
A.H2SO4Na2SO4AgNO3BaCl2.
B.NaOHNa2CO3NaHSO4MgCl2
C.CaCl2NaNO3MgSO4BaCl2
D.HNO3KOHKClK2CO3
请问下有无同学需要H37RA的?我是做EAE模型的,上个月购买了BDDifco公司的H37RA(货号),因为购买的时候只能整盒6支购买,但我们用不了那么多,所以想问问有无同学需要的,100mg/支,800元/支或用等价试剂交换。地址广州。有需要的请私信,谢谢!
2.NaHCO3,Ba(OH)3,H2SO4
3.HCL,NaAlO2,NaHSO4
4.Ca(OH)2,Na2CO3,BaCO3
谢谢了
要原因
C.MgCl2溶液和氨水D.盐酸和NaAlO2溶液
为什么
暂无品牌问答