SolutionΙCellresUSPensionsolution(50mMTris-HCl,pH7.5,10mMEDTA,RNaseA100μg/ml)1MTris-HCl(pH7.6)2.5ml0.25MEDTA2.0mlddH2O45mlsterilebyautoclaveadd1%RNase0.5mlstoreatroomtemperatureSolutionⅡCelllysissolution(0.2NNaOH,1%SDS)Mix0.4NNaOHand2%SDSinsamevolume.SolutionⅢNeutralizationsolution(1.32Mpotassiumacetate,pH5.2)5Mpotassiumacetate13.2mlHac27mladjustpHto5.2addddH2Oto50mlsterilebyautoclavestoreatroomtemperatureRNaseA:10mg/ml溶于TE中,在沸水中煮10-30分钟之后分成小份储存于-20℃