【求助】转录因子表达载体能与荧光素酶报告载体共转染吗
2021-07-29
问题描述：
我想研究转录因子A对B物质表达有无影响，先构建转录因子A的真核表达载体和含B物质全长启动子区的荧光素酶报告质粒，那么转染时细胞是否加1.A表达载体 2.B荧光素酶报告质粒 3.作为reference的内参质粒？请教有哪位高手做过？可以这么做吗？
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mpark
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Before you start these experiments, you need to check NCBI data base to see if the transcription factor A interacts with the promoter B. If there is no such kind data available, you need to run DNA gel retardation assay or DNase I assay to make sure that the transcription factor A interacts with promoter B specifically. Your plan is an important cell-based assay to confirm that the regulation of promoter B transcriptional activities by transcription factor A in tissue cultures.
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maoliping70
用户Thank mpark very much.
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