Description
Product Highlights
RNA was isolated from 20 mg freeze-dried budding leaves of A. thaliana using ISOLATE II RNA Plant Kit. The extracted RNA was diluted in a 2-fold serial dilution (lanes 1-7) and PCR was performed using MyTaq One-Step RT-PCR Kit.The results illustrate the quality of the RNA obtained, as it can be used for very sensitive cDNA synthesis and PCR without further purification.
Product Description
The ISOLATE II RNA Plant Kit provides a simple, efficient column-based method for the isolation of total RNA from a wide variety of plant materials, including leaves, bark, roots and fruits, without the need for hazardous reagents such as phenol.
By combining the stringency of guanidinium thiocyanate lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II RNA Plant Kit provides a fast method for the purification of high-quality total RNA from most plant cells, including plant tissues, leaves, bark, roots and fruits.
Some plant tissues such as maize endosperm and the mycelia of filamentous fungi do not lyse well in guanidinium thiocyanate and can solidify, resulting in poor yields and so guanidinium hydrochloride lysis buffer is also provided as an alternative. Residual amounts of contaminating DNA are selectively removed using a column, however for ultrasensitive applications, remaining DNA can be removed usingRNase-free DNase I that is supplied with the kit.
The ISOLATE II RNA Plant Kit has been designed to deliver optimal performance in RT-qPCR in conjunction with either the SensiFAST cDNA Synthesis Kit and SensiFAST Real-Time PCR Kits, or the SensiFAST One-Step Real-Time RT-PCR Kits. Additionally, the ISOLATE II RNA Plant Kit can be used to purify samples prior to RT-PCR amplification using the Tetro cDNA Synthesis Kit and any enzyme from the Meridian PCR portfolio, including MyTaq DNA Polymerase.
Applications
- RT-qPCR
- End-point RT-PCR
- Northern, dot and slot blotting
- Array analysis
- Poly A+ RNA selection
- RNA-Seq
Nucleic Acid Isolation Guide
Download the ISOLATE II Guide with detailed product descriptions and performance data to help you choose the best product for your researchISOLATE II Selection Chart
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希望知道如何购买的朋友告诉一下，谢谢。
我使用的是北京北方生物技术研究所提供的KIT.盒子里面有睾酮标准品，兔抗睾酮抗体，带碘标记的睾酮，和驴抗兔免疫分离剂。实验过后我查了一下生物化学书，发现睾酮这种激素的化学本质非蛋白质，而是缁醇。这样问题就出来了：据我所知，产生免疫反应的是蛋白质，然而性类固醇激素的化学本质是缁醇。请问各位，缁醇也能产生免疫反应吗？
一般来说，试剂盒曲线上的最小浓度是比较准确的，低于这个值因为与曲线是个“S"型结构有关，所以结果是有偏差的，是一种估算。再加上实验误差，所以达不到理想的效果。建议选择试剂盒时，应该看曲线上最小的浓度值，而不是试剂盒上写的灵敏度。
虽然酶活性调节ELISA方法的灵敏度目前并不十分理想，但在酶活性放大ELISA中，检测的灵敏度远比RIA高。根据质量作用定律。即免疫反应所形成的免疫复合物量与反应物浓度成正比。推测所检测的待测物分子数为1。已知1个摩尔浓度含6.02×1023个分子，那么理论推测酶活性放大ELISA方法的最低检测限可达1.7×10-24mol/L。虽然在实际应用中由于反应条件和试剂纯度以及仪器精度等因素的影响，往往达不到这个水平(大于104个分子)，但表明ELISA在灵敏度方面的改进潜力是很大的。
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