PhageDNAIsolationKit
Thiskitprovidesarapidspincolumnmethodforthe purificationoftotalDNAfromabroadspectrumof bacteriophagespropagatedinbacteriagrowninliquidcultures.TheDNAisisolatedwithouttheuseofphenol,chloroformorcesiumchloridebandingprocedures.Thespin-columnbasedprocedureisrapid andcanbecompletedinlessthan45minutes.Thekitishighlyefficientforprocessingsmallvolumesofphagesupernatant(500µL–1mL)and withtheoptionalDNaseandProteinaseKtreatmentsphageDNAyieldsaremaximizedwhilehostDNAcontaminationisminimized. PurifiedtotalphageDNAisofthehighestintegrity,andcanbeusedinanumberofdownstreamapplicationsincludingPCR,qPCR, RestrictionFragmentLengthPolymorphism(RFLP),sequencing,cloning, SouthernBlotandmore.
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