SequencingReactionClean-UpKit
Thiskitprovidesarapidspincolumnprocedureforthepurificationandclean-upofsequencingandvariousotherenzymaticreactionsincludingrestrictionenzymedigests,Klenowreactions,alkalinephosphatasereactions,andligations.Thekitisusedtoremovereactioncontaminantsincludingdyeterminators,salts,enzymes,excessprimersandprimerdimers.Contaminantsareundesirableastheycaninterferewithmanydownstreamapplicationsincludingsequencing,RFLP,restrictionenzymedigestionsandligation.Purificationisbasedonspin-columnchromatographywithouttheuseofphenol,chloroformoralcoholprecipitation.Thekitprovidesahighqualityproductwithupto90%recovery.
Thekitisalsoavailableina96-wellformatforhigh-throughputsequencingreactionclean-up. Purification withthe96-wellplatecanbeperformedusingeitheravacuummanifoldorcentrifugation.
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