Description:
ThermostableDNAHelicaseisaDNAhelicasethatusestheenergygeneratedbythehydrolysisofnucleosidetriphosphatestobreakthehydrogenbondslinkingthetwostrandstogetherinduplexDNA.Itisactiveinatemperaturerangeof45to65ºC.
SUPPLIEDWITH:
10XAnnealingBufferII
100mMMgSO4
SuppliedbyUsers:ATP
Source:
PurifiedfromanE.colistrainthatoverexpressesthehelicasegeneisolatedfromathermophilicmicroorganism.
Reactioncondition:
1XAnnealingBufferII:
10mMKCl
20mMTris-HCl,pH8.8@25°C
Supplementwith4mMMgSO4and3mMATP.
Incubateat45-65ºC.
StorageConditions:
StorageTemperature:
-20ºC
Concentration:
0.5mg/ml
StorageBuffer:
20mMTris-HClpH7.4
200mMNaCl
1mMDTT
0.1mMEDTA
50%Glycerol
QualityControl:
QualityAssuranceStatement:
ThermostableHelicaseispurifiedfreeofcontaminatingendonucleasesandexonucleases.EachlotistestedforDNAunwindingactivity.
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