Description:
TheT7Gp4AHelicaseexhibitsa5to3helicaseactivityaswellasprimaseactivity.Itishighlyprocessive,travelinganaverageof75kbofsinglestrandedDNAbeforedissociating.
SUPPLIEDWITH:
10XGp4AReactionBuffer
SuppliedbyUsers:dTTP
Source:
PurifiedfromanE.colistrainthatoverexpressesthegp4AgeneisolatedfromtheT7phage.
Reactioncondition:
1XGp4AReactionBuffer:
35mMTrisacetate,pH7.5
11mMmagnesiumacetate
5mMDTT
0.01%TritonX-100
Supplementwith2mMdTTP.
Incubateat25-37ºC.
NOTE:
TheconcentrationofdTTPshouldbeoptimizedforeachspecificapplication.
Fortheprimaseactivity,supplementreactionswithNTPs.
StorageConditions:
StorageTemperature:
-20ºC
Concentration:
1mg/ml
StorageBuffer:
20mMTrisHClpH7.5
100mMNaCl
0.1mMEDTA
1mMDTT
50%Glycerol
pH7.5@RT
QualityControl:
QualityAssuranceStatement:
T7Gp4AHelicaseispurifiedfreeofcontaminatingendonucleasesandexonucleases.EachlotistestedforDNAunwindingactivityinfunctionalactivityassays.
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