货号: | DN25-10MG |
供应商: | 上海善然生物科技有限公司 |
数量: | 现货 |
保存条件: | 低温 |
规格: | DN25-10MG |
A9539-250G | 琼脂糖 |
T1503-5KG | Tris 碱 |
E5134-5KG | EDTA-Na2 |
52365-250G | CTAB |
62476-500G-F | 氯化锂 |
31650-250G | 氯化锌 |
PVP40-500G | PVP40 |
S5881-1KG | 颗粒氢氧化钠 |
217263-250G | 硫代硫酸钠 |
60489-250G-F | 过硫酸钾 |
H3506-500MG | 透明质酸酶 |
P4937-100ML | 细胞分离液 |
E1644-100G | EDTA-Na2 |
产品名称
AnalysisNote
Proteindeterminedbybiuret.
Application
用于从蛋白质样品中除去DNA。
DNAseIisusedtonickDNAasafirststeptoincorporatelabeledbasesintoDNA.TheenzymefromSigmahasbeenusedduringtheisolationofplasmamembranevesiclesfromNeurosporacrassacellculture.1IthasalsobeenusedalongwithtrypsinforthepreparationofsinglecellsUSPensionfromrattestes.2
DeoxyribonucleaseIfrombovinepancreashasbeenusedinastudytocompareseveralproceduresforreducingRNasecontaminationinpreparationsofDNase.DeoxyribonucleaseIfrombovinepancreashasalsobeenusedinastudytoinvestigatetheeffectofthecompositionofsodiumdodecylsulfatepreparationsontherenaturationofenzymesafterpolyacrylamidegelelectrophoresis.
PreparationNote
10mg/mLsolutionofDNAseIin0.15MNaClmaylose<10%ofitsactivitywhenstoredforaweekinaliquotsat−20°C.Thesamesolutionsstoredinaliquotsat2-8°Ccanloseapproximately20%activity.Itremainsactiveforuptofivehoursat60°CbetweenpH5and7,andlosesactivityin<10minutesat68°C.Itlosesactivityattherateof6%/hourinacetatebuffer(pH5.0)andtrisbuffer((pH7.2)at1mg/mLconcentration.
DerivedfromNewZealand-sourcedpancreas
UnitDefinition
在pH5.0和25°C下以I型或III型DNA为底物,一个Kunitz单位每分钟产生的ΔA260为0.001。[Mg2+]=4.2mM。
Physicalform
Crudepreparation,containscalciumchloride
Biochem/physiolActions
DNaseIisanendonucleasethatactsonphosphodiesterbondsadjacenttopyrimidinestoproducepolynucleotideswithterminal5′-phosphates.InthepresenceofMg2+,DNAseIcleaveseachstrandofDNAindependentlyandthecleavagesitesarerandom.BothDNAstrandsarecleavedatapproximatelythesamesiteinthepresenceofMn2+.3DivalentcationssuchasMn2+,Ca2+,Co2+,andZn2+areactivatorsoftheenzyme.Aconcentrationof5mMCa2+stABIlizestheenzymeagainstproteolyticdigestion.ThepHoptimumisfoundtobebetween7and8.4DNAseIfrombovinepancreasconsistsoffourchromatographicallydistinguishablecomponents,A,B,C,andD,withtheirmolarratiosbeing4:1:1respectively.OnlyminoramountsofDarefound.52-Mercaptoethanol,chelators,sodiumdodecylsulfate(SDS)6andactin7areknowntoinhibittheenzymeactivity.
性质
RelatedCategories | 3.1.x.xActingonesters,3.x.x.xHydrolases,ApoptosisandCellCycle,ApplicationIndex,EnzymeClassIndex, |
QualityLevel | PREMIUM |
form | lyophilizedpowder |
molwt | molwt~31 kDa |
composition | Protein,≥85% |
solubility | 0.15MNaCl:soluble5.0 mg/mL,hazy |
FeaturedIndustry | DiagnosticAssayManufacturing DiagnosticAssayManufacturing |
foreignactivity | RNase≤0.02% |