- Description
- Details
- Reviews
Description
Details
Stage-specific embryonic antigen-1 (SSEA-1) is a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma (EC), murine embryonic stem (ES), and murine and human germ (EG) cells. No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. SSEA-1 is associated with cell adhesion, migration and differentiation.
Sample Data Figure A: FC analysis of SSEA-1 Antibody on mouse ES cells at a 1:100 dilution. Green histogram represents SSEA-1 Antibody and open histogram represents isotype control. A PE-conjugated anti-Mouse IgM was used as the secondary antibody. Figure B: ICC analysis on mouse ES cells. Cells were stained with SSEA-1 Antibody at a 1:100 dilution followed by an Alexa Fluor 594-conjugated secondary antibody (red). Nuclei were counterstained with DAPI (blue).
Details
Details
Concentration | 0.5 mg/mL |
---|---|
Species Reactivity | Human, Mouse |
Host | Mouse Monoclonal |
Clone | MC-480 |
Isotype | IgM |
Immunogen | F9 tetracarcinoma stem cells (X-irradiated) |
Formulation | Aqueous buffer, 0.09% sodium azide. |
Storage and Stability | Store at 2-8°C. Stable for 6 months from date of receipt when stored as directed. |
Applications Tested | Flow Cytometry (FC), Immunocytochemistry/Immunofluorescence (ICC/IF) |
Recommended Dilutions | Flow Cytometry 1:100Immunocytochemistry/Immunofluorescence 1:100It is recommended that the antibody be titrated for optimal performance for each application. |
Alternative Names | CD15, Stage-specific embryonic antigen 1 |
References | Brambrink, T., et al. (2008) Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell 2: 151-159. PMID: 18371436Draper, J.S., et al. (2002) Surface antigens of human embryonic stem cells: changes upon differentiation in culture. J Anat 200: 249-258. PMID: 12033729 |
Technical Documents | ST11013 Technical Data Sheet |
Reviews
Write Your Own Review
Tags
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
代谢通路:目前在通路数据库(PATHWAY database) 中代谢通路是建立得最好的,有大约90个参考代谢途径的图形。每个参考代谢途径是一个由酶或EC号组成的网络。
同:均需要转运体蛋白.
异:易化扩散顺浓度梯度或电位梯度运输;两种主动运输逆着电化学梯度运输.
易化扩散不消耗ATP;原发性主动转运能量来源是直接分解ATP;继发性主动转运的能量来自原发性主动转运所形成的离子浓度梯度,间接利用了ATP.
一、原发性主动转运
原发性主动转运 (Primary active transport)是由ATP直接供能的逆浓度差的转运方式。Na+-K+-ATP酶(钠泵)是目前研究得最清楚的原发性主动转运。钠泵是镶嵌在膜脂质双分子层中的特殊蛋白质,除具有对Na+、K+的转运功能外,还具有ATP酶的活性,可分解ATP释放能量,用于Na+、K+的主动转运。因此,钠泵是Na+-K+依赖式的ATP酶,其对Na+、K+的主动转运是由其磷酸化和脱磷酸化循环驱动的,是一种消耗钠泵活动时,泵出细胞Na+和泵入细胞K+两个过程是耦连在一起的,生理条件下,每分解一分子ATP,可使3个Na+被泵出胞外,同时2个K+被泵回胞内(右下图)。
钠泵由α亚单位(催化亚单位)和β亚单位(调节亚单位)组成。胞内Na+与α亚单位结合时,ATP也与之结合并被水解,释放出ADP,使钠泵磷酸化而构象改变,高亲和K+而低亲和Na+,在细胞外一侧释放Na+而结合K+。当胞外K+与α亚单位结合时,钠泵脱磷酸化,返回到原来的构象,高亲和Na+而低亲和K+,在细胞内一侧释放K+而再一次结合Na+,开始下一个循环。
钠泵示意图
Na+泵广泛存在于身体各种细胞的细胞膜上,它们在维持细胞内外正常的Na+、K+浓度差中起重要作用,在此过程中消耗的ATP能是人体代谢产能的1/4,其钠泵的生理意义如下:
①维持细胞内高K+(是胞内许多代谢反应的必需条件);
②防止细胞内Na+过多(从而防止由胞内高渗引起的细胞肿胀);
③最重要的意义是,钠泵活动建立了一种势能贮备(即细胞内外的Na+、K+浓度差),这是可兴奋细胞兴奋性的基础。
除钠泵外,体内还有氢泵、钙泵和碘泵等,均属原发性主动转运。
暂无品牌问答