mEF Feeder Cells (NeoR) Cat. #. SC-001
Description:
BioPioneer"smEF Feeder Cells (NeoR) are specially manufactured mouse embryonic fibroblast (mEF) feeder cells for supporting growth of non-differentiated human and mouse embryonic stem (ES) cells. The mEF Feeder cells are neomycin-resistant, thus, useful for gene targeting applications.
Shipping and Storage Condition:
Ship in dry ice;
Store in liquid nitrogen upon receiving.
Specifications:
Cell Count: 3-5x 106 Cells/tube x 3 tubes
Inactivated: by irradiation
Sterility: no microbial growth detected
Mycoplasma: no mycoplasma growth detected
Antibiotics Resistance: Neomycin resistant
Plating Efficiency: > 70% cells adhere within 24 hours of plating
Monolayer Formation: forms confluent monolayer within 2days of plating
ES Cell Maintenance: supports growth of non-differentiatedhuman and mouse ES cells
Plating Instruction:
Store the cells in Liquid nitrogen upon receiving.Thawing the cells at 37 oC water bath (do not over incubate), immediately transfer to mEF medium, then spin to pellet the cells using a bench-top centrifuge, such as Beckman-Coulter?s ALLEGRA 6R at 800 rpm. Re-suspend the cells with VERY GENTLE pipetting in an appropriate amount (10-20 mL) of a fresh DMEM medium containing 10% FCS, 1 x Antibiotics and 50 ?M ?-Mercaptoethanol and plate the cells on 0.1% gelatin treated plate.
Applications:
? As feeder layer for mouse ES (mES) cells;
? As feeder layer for human ES (hES) cells.
Disclaimer:
Some applications in which this product can be used may be covered by patents issued and applicable in certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.
Limited Product Warranty:
This warranty limits BioPioneer?s liability to replacement of the product. No other warranties of any kind, express or implied, including, without limitation, implied warranties of merchantability or fitness for a particular purpose, are provided by BioPioneer. BioPioneer shall have no liability for any direct, indirect, consequential, or incidental damages arising out of the use, the results of use, or the inability to usethis product.
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【2】信号:信号是数据的电磁编码或电子编码。和数据一样,信号也分为模拟信号和数字信号。模拟信号是指电信号的参量是连续取值的,其特点是幅度连续。常见的模拟信号有电话、传真和电视信号等。数字信号是离散的,从一个值到另一个值的改变是瞬时的,就像开启和关闭电源一样。数字信号的特点是幅度被限制在有限个数值之内。常见的数字信号有电报符号、数字数据等。信号是运载消息的工具,是消息的载体。从广义上讲,它包含光信号、声信号和电信号等。
请问哪种神经细胞可以代替原代海马神经元培养细胞?PC12?HT-22?SH-SY5Y?BV-2一般都适用于什么模型,大家一般用哪个呢
信号通路图:向左转|向右转
相关抑制剂:Selumetinib(AZD6244)是一种有效,高选择性的MEK1抑制剂,IC50为14 nM,也抑制ERK1/2磷酸化,IC50为10 nM,对p38α, MKK6, EGFR, ErbB2, ERK2, B-Raf等没有抑制作用。Phase 3。Vemurafenib(PLX4032, RG7204)是一种新型有效的B-RafV600E抑制剂,IC50为31 nM。SB203580是一种p38 MAPK抑制剂,IC50为0.3-0.5 μM,与SAPK3(106T)和SAPK4(106T)相比选择性低10倍,且抑制PKB磷酸化,IC50为3-5 μM。参考:www.selleck.cn/pharmacological_MAPK.html
→MAPKK→MAPK→进入细胞核→其它激酶或基因调控蛋白(转录因子) 的磷酸化修钸。
信号通路的组成: 配体――生长因子; RTK—酪氨酸;
接头蛋白(生长因子受体接头蛋白-2, GRB-2); GRF--鸟苷酸释放因子; Ras—GTP 结合蛋白; Raf――是丝氨酸/苏氨酸(Ser/Thr)
蛋白激酶(称 MAPKKK)。
主要功能: 调节细胞的增殖与分化, 促进细胞存活, 以及细胞代谢过程中的调节与校正。
我想测药物干预下P65信号通路激活情况,想问下需要像wb一样做一个磷酸化/总蛋白的比值吗,还是分别测出来比较就可以了呢。
细胞信号转导分析的生物信息学方法:
a)选择关键词,从GO数据库中寻找相关基因,比如extracellular表示为分泌蛋白
b)通过GO,BioCarta和Kegg信号通路数据分析给定基因所属的信号通路,功能分类等
c)比较多组基因按功能,通路分组在统计学上的差异,从而得到各组基因的功能差异
d)新信号通路的分析
回复:【资源】很有价值的信号通路分析平台方法!-丁香园论坛
大家好,
我是今年刚毕业找到一份实验室研究工作的小猫,以前常作蛋白合成和分子克隆的,新实验室的老板想要研究些信号通路问题,具体就是研究一个病毒的致病途径是什么,定了大方向后我就扎到了文献的海洋中,可是越看就越有一个问题不明白.
就是我能找到的几乎所有文章,都是用的癌细胞做的模型,比如说hela,就是乳头瘤,293,就是肾瘤,还有SH5Y,就是神经瘤.我不太懂,就是病毒感染人的时候,不是感染的正常细胞吗,用病毒来感染癌细胞,然后在这些癌细胞中检测病毒的作用阿,信号通路之类的,要怎么绕过癌细胞其实和正常细胞不一样这个问题呢?
我看大部分文献都不在这方面作解释说明,仿佛都是约定俗成的用癌细胞,我对信号通路研究也是啥都不懂,两眼抹黑,求各路大神赐教ORZ
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