Storethisstockinadarkvial,refrigerated. Workingsolution Take1ulofeachaddedto1ulofPBS.ThiscanbestoredatRTandcanbemadeeverycoupleofweeks.Dilutecellswithanequalvolumeoftheworkingsolutionandimmediatelylookonthefluorescentmicroscope.Dilutecellsifcellcountistohigh.Remembertotakethisintoaccountwhenyoucalculateoriginalnumbers GreenisLive,RedisDead Note:thesechemicalsarehighlycarcinogenic.PIcanbeusedinplaceofEBatthesameconcentrationThisisamicroscopybasedapplicationfordefinitivediscriminationofliveanddeadcells.TrypanBlueexclusionnotoriouslyoverestimatesthenumberofdeadcells.Usingthisprotocol,youcanobtainamoreaccuratecountofcells.