Cdc25isaproteinphosphataseresponsIBLefordephosphorylatingandactivatingcdc2,acrucialstepinregulatingtheentryofalleukaryoticcellsintotheM-phaseofthecellcycle.Cdc25isphosphorylatedthroughoutinterphasebutnotinmitosis.Theprimarysiteforphosphorylationofcdc25isatSer216.Phosphorylatedcdc25bindstomembersofthe14-3-3familyofproteinswhichpreventsitsactivationofcdc2.Chk1,aproteinkinaseinvolvedintheDNAdamagecheckpointresponseinfissionyeast,hasbeenshowntophosphorylatecdc25atSer216invitro. Contributor:CellSignalingTechnology REFERENCES: