·HiroHirai"sPrimedinSituSynthesis(SchistosomaGenomeNetwork)ThePRINS(Primedinsitu)techniqueusesaspecificprimer,dNTPswithDig-11-dUTPandDNApolymerasetoperformaprimerextensionreactiononachromosomepreparation.TodateithasbeenusedtolocalizethetelomericrepeatelementandtheSmalpharepeatelementonS.mansonichromosomes.ThisprotocolusesthePRINSreactionkitfromBoehringerMannheim,togetherwithTaqDNApolymerasefromGibcoBRL.Reagentsfromothermanufacturersmayworkequallywellbuthavenotbeentested. ·PRINS(AdvancedBiotechnologies)DetailedprotocolforPRINS