商品信息
联系客服
郑重提醒:
无质量问题不接受退换货,下单前请仔细核对信息。
下单后请及时联系客服核对商品价格,订单生效后再付款。
GenWay/Dog CRP (C-Reactive Protein) ELISA Kit/GWB-3261A1/96 well
免费咨询热线
4000-520-616
Description:
ShortDescription:InthisassaytheCRPpresentinserumsamplereactswiththeanti-CRPantibodieswhichhavebeenadsorbedtothesurfaceofpolystyrenemicrotitrewells.Aftertheremovalofunboundserumproteinsbywashing,anti-CRPantibodiesconjugatedwithhorserADIshperoxidase(HRP),areadded.Theseenzyme-labeledantibodiesformcomplexeswiththepreviouslyboundserumCRP.Followinganotherwashingstep,theenzymeboundtotheimmunosorbentisassayedbytheadditionofachromogenicsubstrate,3,3’,5,5’-tetramethylbenzidine(TMB).ThequantityofboundenzymevariesdirectlywiththeconcentrationofCRPinthesampletested;thus,theabsorbance,at450nm,isameasureoftheconcentrationofCRPinthetestsample.ThequantityofCRPinthetestsamplecanbeinterpolatedfromthestandardcurveconstructedfromthestandards,andcorrectedforserumdilution.AdditionalInformation:
Name | DogCRP(C-ReactiveProtein)ELISAKit | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
RelatedProductNames | DogCRP(C-ReactiveProtein)ELISAKit;N/APTX1DogCRP(C-ReactiveProtein)CRP | ||||||||||||||||
Geneid | 488629 | ||||||||||||||||
AliasSymbols | CRP,PTX1 | ||||||||||||||||
ProteinName | Pentaxin | ||||||||||||||||
SwissprotID | T2KEN6 | ||||||||||||||||
SpeciesReactivity | Dog | ||||||||||||||||
AssayInfo | AssayMethodology:QuantitativeSandwichELISA | ||||||||||||||||
Sensitivity | 0.855ng/ml | ||||||||||||||||
KitRange | 3.125ng/ml-200ng/ml | ||||||||||||||||
Homology | Dog | ||||||||||||||||
NCBIAccNumber | NP_000558.2 | ||||||||||||||||
StandardRange | ReferToManual | ||||||||||||||||
SampleType | Serum,plasmaandotherBIOLOGicalfluids | ||||||||||||||||
ProductFormat | InSolution | ||||||||||||||||
Specimen/Volumes | ReferToManual | ||||||||||||||||
SwissProtNumber | P02741 | ||||||||||||||||
Applications | ELISA | ||||||||||||||||
ReconstitutionandStorage | 1.DILUENT The5XDiluentConcentrateisstableuntiltheexpira-tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionsshouldbestoredat4-8C. 2.WASHSOLUTION The20XWashSolutionConcentrateisstableuntiltheexpira¬tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionscanbestoredatroomtemperature(1625C)orat4-8C. 3.ENZYME-ANTIBODYCONJUGATE Undilutedhorseradishperoxidaseanti-CRPconjugateshouldbestoredat4-8Canddilutedimmediatelypriortouse.Theworkingconjugatesolutionisstableforupto8hours. 4.CHROMOGEN-SUBSTRATESOLUTION TheSubstrateSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate. 5.STOPSOLUTION TheStopSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate. 6.ANTI-DOGCRPELISAMICROPLATE Anti-DogCRPcoatedwellsarestableuntiltheexpirationdate,andshouldbestoredat4-8Cinsealedfoilpouchwithdesiccantpack. 7.DOGCRPCALIBRATOR ThelyophilizedDogCRPcalibratorshouldbestoredat4Corfrozenuntilreconstituted.Thereconstitutedcalibratorshouldbealiquotedoutandstoredfrozen(Avoidmultiplefreeze-thawcycles).Theworkingstandardsolutionsshouldbepreparedimmediatelypriortouseandarestableforupto8hours. | ||||||||||||||||
Reactivity | Canine | ||||||||||||||||
KitSize | 96well | ||||||||||||||||
KitPrinciple | GenWayBiotechCRPELISAKit(Dog)(GWB-3261A1)isbasedonstandardsandwichenzyme-linkedimmuno-sorbentassaytechnology.AnantibodyforPentaxinhasbeenpre-coatedontoa96-wellplate(12x8WellStrips).Standardsortestsamplesareaddedtothewells,incubatedandwashedtoremoveunboundproteins.AnAnti-Pentaxin-HRPconjugateddetectorantibodyisthenadded,incubatedandunboundconjugateiswashedaway.AnenzymaticreactionisproducedthroughtheadditionofTMBsubstratewhichiscatalyzedbyHRPgeneratingabluecolorproductthatchangestoyellowafteraddingacidicstopsolution.Thedensityofyellowcolorationreadbyabsorbanceat450nmisquantitativelyproportionaltotheamountofsamplePentaxincapturedinthewell. | ||||||||||||||||
KitComponent |
| ||||||||||||||||
KitDuration | 25Minutes | ||||||||||||||||
Storage | Refertokitinstructionsforcomponentstorage | ||||||||||||||||
LeadTime | Domestic:within1weekdelivery International:1week | ||||||||||||||||
IntendedUse | ResearchUseOnly | ||||||||||||||||
AdditionalInformation | IntendedUse:TheCRPtestkitsareahighlysensitivetwo-siteenzymelinkedimmunoassay(ELISA)formeasuringCRPinDogbiologicalsamples. | ||||||||||||||||
:: | Reagents: 1.DILUENTCONCENTRATE(RunningBuffer) Onebottlecontaining50mlofa5Xconcentrateddiluentrunningbuffer. 2.WASHSOLUTIONCONCENTRATE Onebottlecontaining50mlofa20Xconcentratedwashsolution. 3.ENZYME-ANTIBODYCONJUGATE100X Onevialcontaining150ulofaffinitypurifiedanti-DogCRPantibodyconjugatedwithhorseradishperoxidaseinastABIlizingbuffer. 4.CHROMOGEN-SUBSTRATESOLUTION Onevialcontaining12mLof3,3’,5,5’-tetramethybenzidine(TMB)andhydrogenperoxideincitricacidbufferatpH3.3. 5.STOPSOLUTION Onevialcontaining12ml0.3Msulfuricacid. WARNING:Avoidcontactwithskin. 6.ANTI-DOGCRPELISAMICROPLATE Twelveremovableeight(8)wellmicrowellstripsinwellholderframe.Eachwelliscoatedwithaffinitypurifiedanti-DogCRP. 7.DOGCRPCALIBRATOR OnevialcontainingalyophilizedDogCRPcalibrator. |