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GenWay/Mouse IgM ELISA Kit/GWB-C12BF2/96 well
免费咨询热线
4000-520-616
AdditionalInformation:
Name | MouseIgMELISAKit | ||||||||||||||||
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RelatedProductNames | MouseIgMELISAKit;N/AMouseIgMIGHM | ||||||||||||||||
AliasSymbols | IgmuchainCregion,IgM,IGHM | ||||||||||||||||
ProteinName | PutativeuncharacterizedIg-regionprotein | ||||||||||||||||
SwissprotID | P04219 | ||||||||||||||||
SpeciesReactivity | Mouse | ||||||||||||||||
AssayInfo | AssayMethodology:QuantitativeSandwichELISA | ||||||||||||||||
Sensitivity | 2.786ng/ml | ||||||||||||||||
KitRange | 25ng/ml-800ng/ml | ||||||||||||||||
Homology | Mouse | ||||||||||||||||
StandardRange | ReferToManual | ||||||||||||||||
SampleType | Serum,plasmaandotherBIOLOGicalfluids | ||||||||||||||||
ProductFormat | InSolution | ||||||||||||||||
Specimen/Volumes | ReferToManual | ||||||||||||||||
SwissProtNumber | P01871 | ||||||||||||||||
Applications | ELISA | ||||||||||||||||
ReconstitutionandStorage | 1.DILUENT The5XDiluentConcentrateisstableuntiltheexpira-tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionsshouldbestoredat4-8C. 2.WASHSOLUTION The20XWashSolutionConcentrateisstableuntiltheexpira¬tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionscanbestoredatroomtemperature(1625C)orat4-8C. 3.ENZYME-ANTIBODYCONJUGATE UndilutedhorserADIshperoxidaseanti-IgMconjugateshouldbestoredat4-8Canddilutedimmediatelypriortouse.Theworkingconjugatesolutionisstableforupto8hours. 4.CHROMOGEN-SUBSTRATESOLUTION TheSubstrateSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate. 5.STOPSOLUTION TheStopSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate. 6.ANTI-MOUSEIgMELISAMICROPLATE Anti-MouseIgMcoatedwellsarestableuntiltheexpirationdate,andshouldbestoredat4-8Cinsealedfoilpouchwithdesiccantpack. 7.MOUSEIgMCALIBRATOR LongTermStorage:Uponreceipt,aliquotthecalibratorandstorethemfrozen.Theywillbestableuntilexpirationdate.ShortTermStorage:thecalibratorisstableforupto14daysat4C.Theworkingstandardsolutionsshouldbepreparedimmediatelypriortouseandarestableforupto8hours. | ||||||||||||||||
Reactivity | Mouse | ||||||||||||||||
KitSize | 96well | ||||||||||||||||
KitPrinciple | GenWayBiotechIgMELISAKit(Mouse)(GWB-C12BF2)isbasedonstandardsandwichenzyme-linkedimmuno-sorbentassaytechnology.AnantibodyforPutativeuncharacterizedIg-regionproteinhasbeenpre-coatedontoa96-wellplate(12x8WellStrips).Standardsortestsamplesareaddedtothewells,incubatedandwashedtoremoveunboundproteins.AnAnti-PutativeuncharacterizedIg-regionprotein-HRPconjugateddetectorantibodyisthenadded,incubatedandunboundconjugateiswashedaway.AnenzymaticreactionisproducedthroughtheadditionofTMBsubstratewhichiscatalyzedbyHRPgeneratingabluecolorproductthatchangestoyellowafteraddingacidicstopsolution.Thedensityofyellowcolorationreadbyabsorbanceat450nmisquantitativelyproportionaltotheamountofsamplePutativeuncharacterizedIg-regionproteincapturedinthewell. | ||||||||||||||||
KitComponent |
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KitDuration | 100Minutes | ||||||||||||||||
Storage | Refertokitinstructionsforcomponentstorage | ||||||||||||||||
LeadTime | Domestic:within1weekdelivery International:1week | ||||||||||||||||
IntendedUse | ResearchUseOnly | ||||||||||||||||
AdditionalInformation | IntendedUse:TheIgMtestkitsareahighlysensitivetwo-siteenzymelinkedimmunoassay(ELISA)formeasuringIgMinbiologicalsamplesofmice. | ||||||||||||||||
:: | Reagents: 1.DILUENTCONCENTRATE(RunningBuffer) Onebottlecontaining50mlofa5Xconcentrateddiluentrunningbuffer. 2.WASHSOLUTIONCONCENTRATE Onebottlecontaining50mlofa20Xconcentratedwashsolution. 3.ENZYME-ANTIBODYCONJUGATE100X Onevialcontaining150ulofaffinitypurifiedanti-MouseIgMantibodyconjugatedwithhorseradishperoxidaseinastABIlizingbuffer. 4.CHROMOGEN-SUBSTRATESOLUTION Onevialcontaining12mLof3,3’,5,5’-tetramethybenzidine(TMB)andhydrogenperoxideincitricacidbufferatpH3.3. 5.STOPSOLUTION Onevialcontaining12ml0.3Msulfuricacid. WARNING:Avoidcontactwithskin. 6.ANTI-MOUSEIgMELISAMICROPLATE Twelveremovableeight(8)wellmicrowellstripsinwellholderframe.Eachwelliscoatedwithaffinitypurifiedanti-MouseIgM. 7.MOUSEIgMCALIBRATOR OnevialcontainingMouseIgMcalibrator. |