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GenWay/ELISA ULTRABLOCK Reagent/GWB-Q00227/100 ml
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4000-520-616
AdditionalInformation:
Name | ELISAULTRABLOCKReagent |
---|---|
RelatedProductNames | ELISAULTRABLOCKReagents,ELISASolutions,ELISAULTRABLOCK |
DescriptionofTarget | ELISAUltrablock |
ProductFormat | Readytouse-liquid |
Applications | ELISA* |
ApplicationInfo | ELISA:Neat DirectandIndirect/sandwich |
Format | ReadyToUse |
StABIlity | 18monthsfromdateofdespatch. |
ReconstitutionandStorage | Storeat+4oC. DONOTFREEZE Thisproductshouldbestoredundiluted. |
Storage | Storeat+4oC. DONOTFREEZE Thisproductshouldbestoredundiluted. |
LeadTime | Domestic:within2-3weeksdelivery International:2-3weeks |
IntendedUse | ResearchUseOnly |
AdditionalInformation | Buffersolution:Phosphatebufferedsaline Preservative:0.05%-SodiumAzide |
:: | IntendedUse: GWB-Q00227isahighperformanceELISAblockingbufferforuseinallELISAformatswithhighbackgroundproblems.Theformulationincludesaproprietaryfishextract,molecularstabilizersandanantimicrobialagent.Itisparticularlyusefulinantigen-downandsandwichELISAassayswithmammaliansamples,especiallyhuman,bovineandporcineserum,wherethenon-mammalianblockingproteinsarelesslikelytointeractwithendogenousantibodiesintheserumsample. SuperiorblockingispossIBLeduetothesmallsizeofthemoleculeswhichblocknon-specificbindingsitesontheadsorbedproteinandunoccupiedregionsofthepolystyreneplatesoftenstericallyinaccessibletotrADItionalblockers.Itprovidesalong-termstableenvironmentforcoatingantigenorcaptureantibody.Platescanbeblockedatroomtemperatureandstoredoncedriedforuptoayearat+4C. |
:: | InstructionsforUse: 1.CoatELISAplatewithantibodyorantigenasrequired. 2.Afterincubation,removethecoatingsolutionandwashtheplatex2withwashbuffer. GWB-Q00199canbeusedforthispurpose. 3.Add300-400ulofGWB-Q00227 andincubatefor2-24hours. Useavolumeequaltoorgreaterthanthevolumeofcoatingsolution. 4.Afterremovaloftheblockingbuffer,continuewiththeassayofdrytheplateforlong-termstorageat+4C. |