Description:
ThePerch(Perciformes)VitellogeninELISAKitisasensitivesandwichenzymelinkedimmunosorbentassayforthequantitativedeterminationofvitellogenininserumandmucusofperciformes.
VitellogenindeterminationisoneofthecoreendpointsinscreeningandtestingforendocrinedisruptingchemicalsstandardizedintheOECDGuidlinesforthetestingofchemicalsforestrogenicactivity:
- OECD(2009),TestNo.229
- OECD(2009),TestNo.230
- OECD(2011),TestNo.234
Vitellogenindeterminationisusedinecotoxicologicalstudiestodetermineestrogeniccompoundsinthewater.
Vitellogeninlevelscanbeusedtodeterminethesexandthestatusofsexualmaturationoffish.
Studies
StimulationStudiesusingestrADIol(E2),Ethinyl-Estradiol(EE2,)BisphenolAshowclearincreasesinVitellogeninlevelsinserumandmucus.
Range: 0-80ng/ml
Sensitivity: <0.5ng/ml
Incubationtime: 4.0hours
Samplevolume: 50µl
Samplepreparation:
- Serum:Storefreshserumsamplesimmediatelyaftercollectionat
-20°Corloweruntilassayed. - Mucus:CollectmucusasdescribedintheTECO®MucusCollectionSet(TE1034).Mucus-containingswabscanbestoredseveralmonthsat<-20°C
Referencevalues:
- Serumlevelsareintherangeofµg/mluptomg/ml
- Mucuslevelareintherangeofng/ml
Species:
- FishPerciformes
- Bluegill(Lepomismacrochirus)
- EuropeanPerch(Percafluviatilis)
KitCompostion:
Reagents
Symbol | Description | Format |
1 | 96-wellplatecoatedwithpVTGAntibody 12breakapartstripsof8wells(12x8intotal),inaframe.Readytouse. | 1plate |
S | StandardStock 10x 0.8µg/ml | 1x0.2ml |
C1 | ControlC1,10x ConcentrationseeDataSheet. | 1x0.2ml |
C2 | ControlC2,10x ConcentrationseeDataSheet. | 1x0.2ml |
2 | WashBuffer50x Dilute1:50withdeionizedwater. | 1x30ml |
3 | DilutionBuffer Readytouse. | 1x55ml |
4 | 4: MatrixSolution Readytouse. | 1x7ml |
5 | 5: BiotinylatedAntibody(Biotin-AB). Readytouse. | 1x12ml |
6 | StreptavidinPeroxidaseConjugate(SA-HRPConjugate). Readytouse. | 1x12ml |
7 | TMBSubstrate
| 1x12ml |
8 | StopSolution–1MHCI. 1Mhydrochloricacid,readytouse. | 1x12ml |
I | Kitinstruction | 1x |