Enzyme-linkedImmunosorbentAssays(ELISAs)combinethespecificityofantibodieswiththesensitivityofsimpleenzymeassays,byusingantibodiesorantigenscoupledtoaneasily-assayedenzyme.ELISAscanprovideausefulmeasurementofantigenorantibodyconcentration.Therearetwomainvariationsonthismethod:TheELISAcanbeusedtodetectthepresenceofantigensthatarerecognizedbyanantibodyoritcanbeusedtotestforantibodiesthatrecognizeanantigen.AnELISAisafive-stepprocedure:1)coatthemicrotiterplatewellswithantigen;2)blockallunboundsitestopreventfalsepositiveresults;3)addantibodytothewells;4)addanti-mouseIgGconjugatedtoanenzyme;5)reactionofasubstratewiththeenzymetoproduceacoloredproduct,thusindicatingapositivereaction.TherearemanydifferenttypesofELISAs.OneofthemostcommontypesofELISAis"sandwichELISA." 相关仪器试剂设备