- Proprietary solution of detergents, protease-inhibitors and buffer
- Efficient lysis of bacterial cells for extraction of inclusion body proteins
Cell Lysis Reagent
The Cell Lysis Reagent is designed for the gentle and efficient lysis of bacterial cells in order to assist in the extraction of inclusion body proteins. Cell lysis is accomplished through non-ionic detergent chemical disruption in conjunction with mechanical disruption. The use of a needle and syringe during the procedure helps to reduce viscosity and facilitate purification, producing proteins that are often greater than 95% pure.
Cell Lysis Reagent does not solubilize inclusion bodies. Inclusion Bodies can be solubilized using Norgen"s Inclusion Body Solubilization Reagent (available separately). Norgen"s Inclusion Body Protein Isolation Kits can also be used as complete kits for cell disruption, inclusion body solubilisation and purification using spin column chromatography
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elisa试剂盒回收率是反应待测物在样品分析过程中的损失的程度,损失越少,回收率越高,如果作标液1PPM,就是1毫克/升,而作出标准数据为0.99毫克/升,就是说你的回收率是99%,这个与真实成分有密切的关系,说明方法的准确度。比如水中总无机氯含量测定,样品水中含有无机氯20mg/L,取100mL被测水样品,加入0.1mL浓度为10mg/mL的含无机氯标准样品,测定时忽略体积变化,如果测定出样品中无机氯为2.98mg/L,则认为回收率为99%。向左转|向右转
2、规范包被操作,吸附是否均匀
3、重复性、可靠性
6、是否提供技术
7、适用于血浆、血清、组织匀浆液、细胞培养上清液、尿液等多种类型的样本
8、可检测动物类型是否丰富
9、可检测指标是否齐全
1、是不是获得SCI文献引用;
2、试剂盒是不是具备高灵敏,质量好的;
3、售后服务完全,提供代测服务;
4、后期实验提供全程技术支持!
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