Sensitize a 96-well microtiter plate with purified antigen. Prepare a solution of the purified antigen of interest in phosphate buffer (see recipe below) such 查看更多>
IntroductionQuality is important in all aspects of tissue culture since the quality of materials used i.e. media and other reagents) will affect the quality of 查看更多>
Immunoassays are a powerful technique for detecting and measuring antigens and antibodies. Immunoassays can be classified three ways based on the steps involve 查看更多>
Formalin Fixed Cell PlatesTrypsinize confluent flasksPool and count cellsCentrifuge at 1500 rpm for 10 minutesResuspend to the appropriate concentration in com 查看更多>
You will need the following solutions: PBS (Phosphate-Buffered Saline, Ca/Mg free) :- Solute1x10xNaCl8g80gKCl0.2g2gNa2PO4 (anhydrous)1.15g11.5gKH2PO4 (anhydrou 查看更多>