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GenWay/FAM FLICA CASPASE-1 KIT /GWB-Q00438/100 TESTS
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4000-520-616
Description:
Description:TheFAMFLICACaspase1kitusesaquickandeasymethodtoanalyzeactivecaspasesinapoptoticcells.AdditionalInformation:
Name | FAMFLICACASPASE-1KIT |
---|---|
RelatedProductNames | FAMFLICACASPASE-1KITKits,FAMFLICACASPASE-1KIT |
DescriptionofTarget | FAMFLICATMCASPASE-1KIT |
SpeciesReactivity | Human |
Homology | Human |
ProductFormat | Kit |
Specificity | CASPASE-1 |
Applications | FlowCytometry,Immunofluorescence |
ApplicationData | Thisproducthasbeenreportedtoworkinthefollowingapplications.Thisinformationisderivedfromtestingwithinourlaboratories,peer-reviewedpublicationsorpersonalcommunicationsfromtheoriginators.Pleaserefertoreferencesindicatedforfurtherinformation.Forgeneralprotocolrecommendations. Wherethisproducthasnotbeentestedforuseinaparticulartechniquethisdoesnotnecessarilyexcludeitsuseinsuchprocedures.Suggestedworkingdilutionsaregivenasaguideonly.Itisrecommendedthattheusertitratestheproductforuseintheirownsystemusingappropriatenegative/positivecontrols. FlowCytometry Immunofluorescence |
Format | Kit |
StABIlity | The150XFLICAreconstitutedstockisstableforupto6monthsandmaybethawedtwiceduringthattime. |
ReconstitutionandStorage | Storetheunopenedkit(andeachunopenedcomponent)at+4Cuntiltheexpirationdate.ProtecttheFLICATMreagentfromlightatalltimes.Oncereconstituted,the150XFLICATMstockshouldbestoredat20Cprotectedfromlight. |
Storage | Storetheunopenedkit(andeachunopenedcomponent)at+4oCuntiltheexpirationdate. ProtecttheFLICAreagentfromlightatalltimes. Oncereconstituted,the150XFLICAstockshouldbestoredat-20Cprotectedfromlight. |
LeadTime | Domestic:within2-3weeksdelivery International:2-3weeks |
IntendedUse | ResearchUseOnly |
KeyReference | References: 1.Hoegen,T.etal.(2011)TheNLRP3InflammasomeContributestoBrainInjuryinPneumococcalMeningitisandIs ActivatedthroughATP-DependentLysosomalCathepsinBRelease.JImmunol.187:5440-51. |
AdditionalInformation | ProductInformation: TheFAMFLICATMCaspase1kitusesaquickandeasymethodtoanalyzeactivecaspasesinapoptoticcells. TestPrinciple: CaspaseFLICATMkitsmeasureapoptosisbydetectingactivecaspasesinwhole,livingcells.ThesekitsdonotworkbyusingantibodiesorasanELISA.Instead,theirmethodologyisbasedonauniquecell-permeableandnon-cytotoxicreagentcalledtheFluorochromeInhibitorofCaspases(FLICA).TheFLICATMreagentcontainsacaspaseinhibitorsequencelinkedtoagreen(Carboxyfluorescein,FAM)fluorescentprobe. TheCaspaseFLICA TM KitsaresuitableforcellsinsUSPension,adherentcells,thintissuesections(butnotfixedor paraffin-embeddedcells)frommanyspeciesincludingmammalian,insectandyeast.Differentcelltypes,e.g.HeLa, primaryneurons,macrophagesandlymphocyteshavealsobeensuccessfullystudiedwiththesekits. Thiskitcanbeusedwithaflowcytometer,fluorescencemicroscopeorafluorescenceplatereaderusingblack microtitreplates. ReagentsInTheKit: 4vialsofFAM-YVAD-FMKFLICATM Reagent-lyophilized 10XWashBuffer,60mL Fixative,6mL PropidiumIodide,1mL HoechstStain,1mL |
:: | ShelfLife: The150XFLICATMreconstitutedstockisstableforupto6monthsandmaybethawedtwiceduringthattime. Acknowledgements: FLICATMisatrademarkofImmunochemistryTechnologies,LLC. |