Technical Documents
Description
Glen-Pak™ DNA and RNA cartridges have advantages over Poly-Pak cartridges in that a single loading of the diluted crude deprotection solution is all that is necessary. Also, the range of purification has been extended to 100+ using DMT-on oligos. Glen-Pak cartridges have similar performance to Fluoro-Pak cartridges but without the need for the fluorous DMT group at the 5" terminus and special phosphoramidites, so the cost is lower. In addition, Glen-Pak cartridges allow purification of virtually the complete range of dyes and modifiers. The Glen-Pak DNA Cartridge 3g is a large cartridge capable of purifying 10-20 µmole oligonucleotide syntheses using the standard DMT-on procedure and Glen-Pak DNA 30mg 96-Well Plates are for parallel purification of up to 50 nmole scale syntheses. The Glen-Pak DNA 3mg 384-Well Plate is designed for use with 384-well plate compatible vacuum manifold systems and can purify up to a 20 nmole scale synthesis. Each well contains 3mg of Glen-Pak DNA resin, which binds about 15 nmoles of full length 40-mer DMT-ON oligo. Scale suggestions for the Glen-Pak DNA product line are shown below:
Glen-Pak DNA Product | Catalog Number | Synthesis Scale Compatibility | |
Glen-Pak DNA 50mg Purification Cartridge | 60-5000-96 | 0 nmole - 200 nmole | |
Glen-Pak DNA Purification Cartridge | 60-5100-XX and 60-5200-XX | 10 nmole - 1.0 µmole | |
Glen-Pak DNA Cartridge 3G | 60-5300-01 | 5 µmole - 20 µmole | |
Glen-Pak DNA 30 mg 96-Well Plate | 60-5400-01 | 10 nmole - 50 nmole | |
Glen-Pak DNA 3mg 384-Well Plate | 60-5500-xx | up to 20 nmoles |
A User Guide to Glen-Pak™ Purification describes in detail the process and several applications for DNA and RNA purification.This booklet is available online at: https://www.glenresearch.com/media/productattach/g/l/glen-pak_2.9_1.pdf.
Details
Specifications | |
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Storage | Controlled room temperature |
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亲子鉴定?
ID,promega.我们实验室主要用的是这两种,由于怕有突变还会备有凯杰的。
如果测序的话,那么就是AB的bigdye吧?
有什么问题我们可以交流。
2 单细胞测序是指单个细胞水平上对基因组进行测序。
3、靶向测序步骤为 样品准备、探针/引物设计、目标序列捕获、文库制备、上机测序、数据分析。
4、单细胞的分离--DNA/RNA的提取和扩增(全基因组扩增和全转录组扩增)---测序以及后续的分析和应用。
相同:使用二代测序
不同:样本处理,文库构建,检测的区域,数据分析流程
暂无品牌问答