The Bcl-2 gene family encodes a set of proteins involved in apoptotic inhibition, which are commonly analyzed during the performance of a Bcl-2 apoptosis assay. The ApoPrimer Set (Bcl-2 family) for RT-PCRis composed of a set of primers used toamplify cDNA derived from the mRNA of seven Bcl-2 family members (mcl-1, bfl-1, bax-alpha, bcl-2, bak, bik, bcl-x), as well as a beta-actin control. A positive control RNA is also included. The beta-actin primer is a useful internal control for sample quantification and for comparison between samples. PCR products generated from the positive control RNA can be distinguished from target mRNA due to size differences of the amplified products.
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做完实验之后放8联排的卡槽怎么按都弹不出来了。求助各位可能的原因有哪些?原因越详细越多越好,避免下次再出现这种状况,有没有其他战友遇到过这种情况?谢谢!
各位大神,有使用过ThermoPX2这个型号的pcr扩增仪吗?
PCR技术类似于DNA的天然复制过程,其特异性依赖于与靶序列两端互补的寡核苷酸引物,由变性--退火--延伸三个基本反应步骤构成
普通PCR的孔所有温度是一样的。
请问PCR仪设置加样量有什么作用,我们实验室有的pcr仪可以设置有的不能设置。如果我用了25微升体系,pcr仪设置时设置了50微升体系会怎么样呢?帮忙回答一下。谢谢~
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