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Description
Data
siRNA Design
Citations
- High siRNA transfection efficiency
- Functional gene silencing post siRNA delivery
- Compatibility with diverse growth conditions (with and without serum)
- Low cytotoxicity
- Easy-to-use protocols for both adherent and suspension cells
Contents
- Hydrated GeneSilencer Lipid
- siRNA Diluent (corresponding ratio for each kit)
Storage
GeneSilencer siRNA Transfection Reagent is shipped at room temperature, as similar to other gene therapy products. For maximum stability, store all reagents at 4篊 upon receipt. If stored properly, all components are stable for 6 months.GeneSilencer Transfection Reagent Kit
- 50 rxn kit, 0.18 ml, T500020
- 200 rxn kit, 0.75 ml, T500750
- 1000 rxn kit, 5 x 0.75 ml, T505750
- 10 rxn sample kit, 0.03 ml, T500020S
Long Noncoding RNA pancEts-1 Promotes Neuroblastoma Progression through hnRNPK-Mediated β-Catenin Stabilization.Li, D.,et al (2018) Cancer Res. 78 (5): 1169-1183. Link
Apoptotic cells trigger the ABCA1/STAT6 pathway leading to PPAR‐γ expression and activation in macrophages.Kim, M-J.,et al (2018) J. Leukocyte Biol. 103(5): 885-895. Link
Transient Silencing of DNA Repair Genes Improves Targeted Gene Integration in the Filamentous Fungus Trichoderma reesei.Chum, P.Y.,et al ((2017) Appl. Environ. Microbiol. 83(15): e00535-17. Link
Programming of macrophages by apoptotic cancer cells inhibits cancer progression through exosomal PTEN and PPARγ ligands.Kim, Y-B.,et al (2017) bioRxiv 217562: 1-75. Link
Role of Sphingosine Kinase 1 and S1P Transporter Spns2 in HGF-mediated Lamellipodia Formation in Lung Endothelium.Fu, P.,et al (2016) J. Biol. Chem. 291(53): 27187?27203. Link
Ubiquitin-specific Protease 20 Regulates the Reciprocal Functions of β-Arrestin2 in Toll-like Receptor 4-promoted Nuclear Factor κB (NFκB) Activation.Jean-Charles, P-Y., (2016) J. Biol. Chem. 291 (14): 7450 ?7464. Link
Tolerogenic nanoparticles inhibit T cell杕ediated autoimmunity through SOCS2.Yeste, A.,et al (2016) Sci. Signal. 9(433): RA61. Link
Synthesis of an Endogenous Steroidal Na Pump Inhibitor Marinobufagenin, Implicated in Human Cardiovascular Diseases, Is Initiated by CYP27A1 via Bile Acid Pathway.Fedorova, O.V.,et al (2015) Circ Cardiovasc Genet. 8(5): 736-45. Link
Thioredoxin Activates MKK4-NFκB Pathway in a Redox-dependent Manner to Control Manganese Superoxide Dismutase Gene Expression in Endothelial Cells.Kundumani-Sridharan, V.,et al (2015) J. Biol. Chem. 290(28): 17505?17519. Link
Identification, Mechanism of Action, and Antitumor Activity of a Small Molecule Inhibitor of Hippo, TGF-β, and Wnt Signaling Pathways.Basu, D.,et al (2014) Mol. Cancer Ther. 13(6): 1457-1467. Link
PKC Potentiates Tyrosine Kinase Inhibitors STI571 and Dasatinib Cytotoxic Effect.Tob蚾, A.,et al (2014) Anticancer Res. 34(7): 3347-3356. Link
TREK2 Expressed Selectively in IB4-Binding C-Fiber Nociceptors Hyperpolarizes Their Membrane Potentials and Limits Spontaneous Pain.Acosta, C.,et al (2014) J. Neurosci. 34(4): 1494-1509. Link
RhoA/Phosphatidylinositol 3-Kinase/Protein Kinase B/Mitogen-Activated Protein Kinase Signaling after Growth Arrest朣pecific Protein 6/Mer Receptor Tyrosine Kinase Engagement Promotes Epithelial Cell Growth and Wound Repair via Upregulation of Hepatocyte Growth Factor in Macrophages.Lee, Y-J.,et al (2014) J. Pharma. Exp. Thera. 350(3): 563-577. Link
GeneSilencer Cell Line Citations: 3T3-L1, A549, A7, AGS, ASM Transformed, B16-F1, BEL7404, Bronchial C166, C2C12, C6R, CHO-K1, DC2.4, GS-KB-3-1, H22, H441, H460, H9c2, hCMEC/D3, HCP40, HCT-116, HEK 293, HEK 293T, HEK-293, HeLa, HepG2, HL-60, HMEC-1, HPAEC, HT29, HUT-78, JAR, Jurkat, KATOIII, L/Stab-2, LNCaP, M2, McA-RH 7777, MCF-7, MDA MB-231, MDA MB-468, MDA-MB-435, MG63, MKN 28, MKN 45, MV-4-11, Neuro2a, NIH-3T3, OK, PC-12, Cortical Neurons, Hippocampal Neurons, Macrophage, Pulmonary Artery, Endothelium, Dendritic Cells(Bone Marrow), Cortical Neurons, Coronary Artery Endothelial, Peritoneal Macrophage, Dorsal Root Ganglion Neurons, Retinal Ganglion, Dorsal Root Ganglion, Schwann Cells Cardiac Fibroblast, Cardiac Myocytes (Neonatal), Lung Microvascular Endothelial Cells, Aortic Muscle Cells, Dendritic Cells, Cerebellar Neurons, Osteoblast, HMVEC, Cardiac Myocytes, T-Cells (CD3+), Cortical Neurons, Dendritic Cells, Cortical Neurons, Aveolar Type II Cells, Macrophage, Dorsal Root Ganglion Lacrimal Gland Acinar Cells, Bone Marrow, Dendritic Cells, Pulmonary Artery Endothelium, Primary HPAEC. RAW 264.7, Renca, SaOs2, Saos-2, SCCH196, SNB19, SW480, U20S
GeneSilencerTM Reagent vs. siPORTTM NeoFXTM Reagent
HEK 293 Cells (click on images to enlarge)
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Figure Legend: HEK 293 cells were transfected with 5, 10, 30 or 50 nM of Silencer FAM Labeled GAPDH siRNA with either GeneSilencer (Genlantis) or siPORT NeoFX (Ambion) siRNA transfection reagents according to the manufacturer"s recommended protocols. Cells were incubated for 48 hours then fixed, permeablized, and incubated with AlexaFluor 555 phalloidin (Invitrogen), which stains cellular actin red. Cells were then mounted on slides and stained with DAPI (Invitrogen), which stains the cell nuclei blue. Transfected cells were visualized by fluorescence microscopy using identical exposure times for FITC, TRITC, and DAPI. The transfected GAPDH siRNA is localized in the cell cytosol and can be seen as green fluorescent specks or dots.
HeLa S3 Cells (click on images to enlarge)
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Figure Legend: HeLa S3 cells were transfected with 5, 10, 30 or 50 nM of Silencer FAM labeled GAPDH siRNA with either GeneSilencer (Genlantis) or siPORT&tm; NeoFX&tm; (Ambion) siRNA transfection reagents according to the manufacturer"s recommended protocols. Cells were incubated for 48 hours then fixed, permeablized, and incubated with AlexaFluor 555 phalloidin (Invitrogen), which stains cellular actin red. Cells were then mounted on slides and stained with DAPI (Invitrogen), which stains the cell nuclei blue. Transfected cells were visualized by fluorescence microscopy using identical exposure times for FITC, TRITC, and DAPI. The transfected GAPDH siRNA is localized in the cell cytosol and can be seen as green fluorescent specks or dots.
How to Design siRNA (Small Interfering RNA)
The sequence of the siRNA can be selected as follows:
The sequence selection process has no other constraints. It is important to note that structure within thetargeted mRNA appears to have minimal effect on the availability of the mRNA target and efficacy of thesiRNA silencing approach. To-date, successful silencing has been achieved using the above method toselect the target sequence, although the method is essentially random with respect to accounting for mRNAstructure.Although siRNA silencing appears to be extremely effective by selecting a single target in the mRNA, itmay be desirable to design and employ two independent siRNA duplexes to control for specificity of thesilencing effect. This recommendation is only for specificity for it is yet unknown if the targeting of a geneby two different siRNA duplexes would be more effective than using a single siRNA duplex. It is believedthat the rate-limiting component of the siRNA effect is the availability of cellular nuclease components andnot mRNA target availability. Therefore, doubling the number of siRNA duplexes is not expected to doublethe rate or efficiency of silencing.If the selected siRNA duplex(es) do not function for silencing, the following steps are recommended. First,a search is conducted for sequencing errors in the gene and possible polymorphisms. Initial studies on thespecificity of target recognition by siRNA duplexes indicates that a single point mutation located in thepaired region of an siRNA duplex is sufficient to abolish target mRNA degradation. Second, a reexaminationis performed to confirm whether the cell line is from the expected species. Third, a secondand/or third target are selected and the corresponding siRNA duplexes prepared.
| Cell Line | Cell Type | Source | Genlantis Product | Citation |
| 3T3-L1 | Embryo | Swiss Mouse | GeneSilencer siRNA Transfection Reagent | Mizuarai, S., Miki, S., Araki, H., Takahashi, K. and Kotani, H.(2005) Identification of Dicarboxylate Carrier Slc25a10 as Malate Transporter in de Novo Fatty Acid Synthesis. J. Biol. Chem.280(37): p. 32434-32441. |
| A549 | Lung Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Ito, K., Yamamura, S., Essilfie-Quaye, S., Cosio, B., Ito, M., Barnes, P.J. and Adcock, I.M. (2006) Histone deacetylase 2-mediated deacetylation of the glucocorticoid receptor enables NFκB suppression. J. Exp. Med. 203(1): 7-13. |
| A549 | Lung Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Stepulak, A., Sifringer, M., Rzeski, W., Endesfelder, S., Gratopp, A., Pohl, E.E., Bittigau, P., Hansen, H.H., Stryjecka-Zimmer, M., Turski, L. and Ikonomidou, C. (2005) NMDA antagonist inhibits the extracellular signal-regulated kinase pathway and suppresses cancer growth. Proc. Natl. Acad. Sci. 102: 15605 - 15610. |
| A7 | Melanoma | Human | GeneSilencer siRNA Transfection Reagent | Scott, M.G.H., Pierotti, V., Storez, H., Lindberg, E., Thuret, A., Muntaner, O., Labbe-Jullie, C., Pitcher, J.A. and Marullo, S. (2006) Cooperative Regulation of Extracellular Signal-Regulated Kinase Activation and Cell Shape Change by Filamin A and β-Arrestins. Mol. & Cell Biol, 26: 3432-3445. |
| AGS | Gastric Epithelium | Human | GeneSilencer siRNA Transfection Reagent | Nagasako, T., Sugiyama, T., Mizushima, T., Miura, Y., Kato, M. and Asaka, M. (2003) Up-regulated Smad5 Mediates Apoptosis of Gastric Epithelial Cells Induced by Helicobacter pylori Infection. J. Biol. Chem.: 278: 4821 - 4825. |
| AGS | Gastric Epithelium | Human | GeneSilencer siRNA Transfection Reagent | Varro, A., Noble, P-J. M., Pritchard, D.M., Kennedy, S., Hart, C.A., Dimaline, R. and Dockray, G.J. (2004) Helicobacter pylori Induces Plasminogen Activator Inhibitor 2 in Gastric Epithelial Cells through Nuclear Factor-B and RhoA: Implications for Invasion and Apoptosis Cancer Res. 64: 1695 - 1702. |
| ASM Transformed | Airway Smooth Muscle Cells | Human | GeneSilencer siRNA Transfection Reagent | Tran, T., Ens-Blackie, K., Rector, E.S., Stelmack, G.L., McNeill, K.D., Tarone, G., Gerthoffer, W.T., Unruh, H. and Halayko, A.J. (2007) Laminin-binding Integrin α7 is Required for Contractile Phenotype Expression by Human Airway Myocyte. Am. J. Respir. Cell Mol. Biol. 37(6): 668-80. |
| B16-F1 | Melanoma | Mouse | GeneSilencer siRNA Transfection Reagent | Bertling, E., Hotulainen, P., Mattila, P.K., Matilainen, T., Salminen, M., and Lappalainen, P. (2004) Cyclase-associated-protein 1 (CAP1) promotes cofilin-induced actin dynamics in mammalian nonmuscle cells. Mol. Biol. Cell 15 (5): p. 2324-2334. |
| B16-F1 | Melanoma | Mouse | GeneSilencer siRNA Transfection Reagent | Hotulainen, P., Paunola, E., Vartiainen, M.K. and Lappalainen, P. (2005) Actin-depolymerizing Factor and Cofilin-1 Play Overlapping Roles in Promoting Rapid F-Actin Depolymerization in Mammalian Nonmuscle Cells. Mol. Biol. Cell. 16(2): p. 649-664. |
| BEL7404 | Hepatoma | Human | GeneSilencer siRNA Transfection Reagent | Liang, X-J, Finkel, T., Shen, D-W, Yin, J-J, Aszalos, A. and Gottesman, M.M. (2008) SIRT1 Contributes in Part to Cisplatin Resistance in Cancer Cells by Altering Mitochondrial Metabolism. Mol Cancer Res 6(9): 1499-506. |
| Bronchial | Smooth Muscle | Human | GeneSilencer siRNA Transfection Reagent | Nunes, R.O., Schmidt, M., Dueck, G., Baarsma, H., Halayko, A.J., Kerstjens, H.A.M., Meurs, H. and Gosens, R. (2008) GSK-3/β-catenin signaling axis in airway smooth muscle: role in mitogenic signaling Am J Physiol Lung Cell Mol Physiol. 294: L1110 - L1118. |
| C166 | Yolk Sack Endothelium | Mouse | GeneSilencer siRNA Transfection Reagent | Zhou, X., Stuart, A., Dettin, L.E., Rodriguez, G., Hoel, B. and Gallicano G.I. (2004) Desmoplakin is required for microvascular tube formation in culture. J. Cell Sci. 117: 3129-3140. |
| C2C12 | Myoblast | Mouse | GeneSilencer siRNA Transfection Reagent | Evangelisti, C., Tazzari, P.L., Riccio, M., Fiume, R., Hozumi, Y., Fala, F., Goto, K.., Manzoli, L., Cocco, L. and Martelli, A.M. (2007) Nuclear diacylglycerol kinase-ζ is a negative regulator of cell cycle progression in C2C12 mouse myoblasts. FASEB J. 21: 3297 - 3307 |
| C6R | Glioma | Rat | GeneSilencer siRNA Transfection Reagent | Saarikangas, J., Hakanen, J., Mattila, P.K., Grumet, M., Salminen, M. and Lappalainen, P. (2008) ABBA regulates plasma-membrane and actin dynamics to promote radial glia extension. J Cell Sci. 121(Pt 9):1444-54. |
| CHO-K1 | Ovary | Chinese Hamster | GeneSilencer siRNA Transfection Reagent | Kheifets, V., Bright, R., Inagaki, K., Schechtman, D. and Mochly-Rosen, D. (2006) Protein Kinase C δ PKC-Annexin V Interaction: a required step in δ PKC translocation and function. J. Biol. Chem. 281(32): p. 23218-23226. |
| DC2.4 | Bone Marrow | Mouse | GeneSilencer siRNA Transfection Reagent | Jing, H., Yen, J-H and Ganea, D. (2004) A novel signaling pathway mediates the inhibition of CCL3/4 expression by PGE2. J. Biol. Chem. 279(53): p. 55176-55186. |
| GS-KB-3-1 | Epidermoid Adenocarcinoma | Human | GeneSilencer siRNA Transfection Reagent | Liang, X-J, Finkel, T., Shen, D-W, Yin, J-J, Aszalos, A. and Gottesman, M.M. (2008) SIRT1 Contributes in Part to Cisplatin Resistance in Cancer Cells by Altering Mitochondrial Metabolism. Mol Cancer Res 6(9): 1499-506. |
| H22 | Hepatoma | Mouse | GeneSilencer siRNA Transfection Reagent | Huang, B., Lei, Z., Zhang, G-M, Li, D., Song, C., Li, B., Liu, Y., Yuan, Y., Unkeless, J., Xiong, H. and Feng, Z-H (2008) SCF-mediated mast cell infiltration and activation exacerbate the inflammation and immunosuppression in tumor microenvironment. Blood 112(4): 1269-79. |
| H22 | Hepatoma | Mouse | GeneSilencer siRNA Transfection Reagent | Huang, B., Zhao, J., Shen, S., Li, H., He, K-L, Shen, G-X, Mayer, L., Unkeless, J., Li, D., Yuan, Y., Zhang, G.-M., Xiong, H. and Feng, Z.-H. (2007) Listeria monocytogenes Promotes Tumor Growth via Tumor Cell Toll-Like Receptor 2 Signaling. Cancer Res. 67(9): p. 4346-4352. |
| H441 | Lung Adenocarcinoma | Human | GeneSilencer siRNA Transfection Reagent | Zhang, Y-A., Nemunaitis, J., Samuel, S.K., Chen, P., Shen, Y. and Tong, A.W. (2006) Antitumor Activity of an Oncolytic Adenovirus-Delivered Oncogene Small Interfering RNA. Cancer Res. 66(19): 9736-9743. |
| H460 | Lung Cancer | Human | GeneSilencer siRNA Transfection Reagent | Ren, J., Shi, M., Liu, R., Yang, Q-H., Johnson, T., Skarnes, W.C. and Du, C. (2005) The Birc6 (Bruce) gene regulates p53 and the mitochondrial pathway of apoptosis and is essential for mouse embryonic development. Proc. Natl. Acad. Sci. USA. 102(3): p. 565-570. |
| H9c2 | Myoblast | Rat | GeneSilencer siRNA Transfection Reagent | Saotome, M., Safiulina, D., Szabadkai, G., Das, S., Fransson, A., Aspenstrom, P., Rizzuto, R. and Hajnoczky, G. (2008) Bidirectional Ca2+-dependent control of mitochondrial dynamics by the Miro GTPase. Proc Natl Acad Sci U S A 105(52): p. 20728. |
| hCMEC/D3 | Brain Microvascular Endothelia | Human | GeneSilencer siRNA Transfection Reagent | Huang, W., Eum, S.Y., Andras, I.E., Hennig, B. and Toborek, M. (2009) PPAR{alpha} and PPAR{gamma} attenuate HIV-induced dysregulation of tight junction proteins by modulations of matrix metalloproteinase and proteasome activities. FASEB J 13 Jan 2009. |
| HCP40 | Colon adenocarcinoma | null | GeneSilencer siRNA Transfection Reagent | Yao, K., Shida, S., Selvakumaran, M., Zimmerman, R., Simon, E., Schick, J., Haas, N.B., Balke, M., Ross, H., Johnson, S.W. and O"Dwyer, P.J. (2005) Macrophage Migration Inhibitory Factor Is a Determinant of Hypoxia-Induced Apoptosis in Colon Cancer Cell Lines. Clin Cancer Res. 11 (20): 7264 - 7272. |
| HCT-116 | Colon Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Wang, J., Rajput, A., Kan, J.L.C., Rose, R., Liu, X., Kuropatwinski, K., Hauser, J., Beko, A., Dominquez, I., Sharratt, E.A., Brattain, L., LeVea, C., Sun, F., Keane, D.M., Gibson, N.W. and Michael G. Brattain (2009) Knockdown of Ron kinase inhibits mutant PI3 kinase and reduces metastasis in human colon carcinoma. J. Biol. Chem. 284 (16) 10912-10922. |
| HCT-116 | Colon Adenocarcinoma | Human | GeneSilencer siRNA Transfection Reagent | Yao, K., Shida, S., Selvakumaran, M., Zimmerman, R., Simon, E., Schick, J., Haas, N.B. Balke, M., Ross, H., Johnson, S.W. and O"Dwyer, P.J. (2005) Macrophage Migration Inhibitory Factor Is a Determinant of Hypoxia-Induced Apoptosis in Colon Cancer Cell Lines. Clin. Can. Res. 11: 7264-7272. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Ahn, S., Nelson, C.D., Garrison, T.R., Miller, W.E. and Lefkowitz, R.J. (2003) Desensitization, internalization, and signaling functions of -arrestins demonstrated by RNA interference. Proc. Natl. Acad. Sci. 100: 1740 - 1744. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Ahn, S., Shenoy, S.K., Wei, H. and Lefkowitz, R.J. (2004) Differential Kinetic and Spatial Patterns of {beta}-Arrestin and G Protein-mediated ERK Activation by the Angiotensin II Receptor. J. Biol. Chem. 279 (34): 35518-35525. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Ahn, S., Wei, H., Garrison, T.R. and Lefkowitz, R.J. (2004) Reciprocal Regulation of Angiotensin Receptor-activated Extracellular Signal-regulated Kinases by (beta)-Arrestins 1 and 2. J. Biol. Chem. 279: 7807-7811. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Khundmiri, S.J., Dean, W.L., McLeish, K.R. and Lederer, E.D. (2005) Parathyroid Hormone-mediated Regulation of Na+-K+-ATPase Requires ERK-dependent Translocation of Protein Kinase C{alpha}. J. Biol. Chem. 280(10): p. 8705-8713. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Kim, J., Ahn, S., Ren, X-R., Whalen, E.J., Reiter, E., Wei, H. and Lefkowitz, R.J. (2005) Functional antagonism of different G protein-coupled receptor kinases for {beta}-arrestin-mediated angiotensin II receptor signaling. Proc. Natl. Acad. Sci.驴102: 1442 - 1447. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Kohout, T.A., Nicholas, S.L., Perry, S.J., Reinhart, G., Junger, S. and Struthers, R. (2004) Differential Desensitization, Receptor Phosphorylation, {beta}-Arrestin Recruitment, and ERK1/2 Activation by the Two Endogenous Ligands for the CC Chemokine Receptor 7. J. Biol. Chem. 279 (22): p. 23214-23222. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Liang, Y., Yu, W., Li, Y., Yan, X., Huang, Q. and Zhu, X. (2004) Nudel functions in membrane traffic mainly through association with Lis1 and cytoplasmic dynein. J. Cell Biol. 164 (4): 557-566. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Ren, X-R., Reiter, E., Ahn, S., Kim, J., Chen, W. and Lefkowitz, R.J. (2005) Different G protein-coupled receptor kinases govern G protein and {beta}-arrestin-mediated signaling of V2 vasopressin receptor. Proc. Natl. Acad. Sci. 102: 1448 - 1453. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Shenoy, S.K., Drake, M.T., Nelson, C.D., Houtz, D.A., Xiao, K., Madabushi, S., Reiter, E., Premont, R.T., Lichtarge, O. and Lefkowitz, R.J. (2006) {beta}-Arrestin-dependent, G Protein-independent ERK1/2 Activation by the {beta}2 Adrenergic Receptor. J. Biol. Chem. 281(2): 1261-1273. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Shikama, Y., Yamada, M., Miyashita, T. (2004) Caspase-8 and caspase-10 activate NF-B through RIP, NIK and IKK kinases. Eur. J. Immun. 33 (7): 1998-2006. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Wei, H., Ahn, S., Barnes, W.G. and Lefkowitz, R.J., (2004) Stable interaction between beta -arrestin2 and AT 1A receptor is required for beta -arrestin2 mediated activation of extracellular signal-regulated kinase 1 and 2. J. Biol. Chem. 279: 48255-48261. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Wei, H., Ahn, S., Shenoy, S.K., Karnik, S.S., Hunyady, L., Luttrell, L.M. and Lefkowitz, R.J. (2003) Independent -arrestin 2 and G protein-mediated pathways for angiotensin II activation of extracellular signal-regulated kinases 1 and 2. Proc. Natl. Acad. Sci. 100: 10782 - 10787. |
| HEK 293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Wu, J-H, Goswami, R., Kim, L.K., Miller, W.E., Peppel, K. and Freedman, N.J. (2005) The Platelet-derived Growth Factor Receptor-{beta} Phosphorylates and Activates G Protein-coupled Receptor Kinase-2: a Mechanism for Feedback Inhibition. J. Biol. Chem. 280(35): p. 31027-31035. |
| HEK 293T | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Wei, H., Ahn, S., Shenoy, S.K., Karnik, S.S., Hunyady, L., Luttrell, L.M. and Lefkowitz, R.J. (2003) Independent -arrestin 2 and G protein-mediated pathways for angiotensin II activation of extracellular signal-regulated kinases 1 and 2. Proc. Natl. Acad. Sci. 100: 10782 - 10787. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Ahn, S., Kim, J., Hara, M.R., Ren, X. and Lefkowitz, R.J. (2009) β -arrestin2 mediates anti-apoptotic signaling through regulation of bad phosphorylation. J Biol Chem 284(13): 8855-65. |
| HEK-293 | Embryonic Fibroblast | Human | GeneSilencer siRNA Transfection Reagent | Barthet, G., Framery, B., Gaven, F., Pellissier, L., Reiter, E., Claeysen, S., Bockaert, J. and Dumuis, A. (2007) 5-Hydroxytryptamine 4 Receptor Activation of the Extracellular Signal-regulated Kinase Pathway Depends on Src Activation but Not on G Protein or beta-Arrestin Signaling. Mol. Biol. Cell. 18(6): 1979-1991. |
| HEk-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | El-Shewy, H.M., Johnson, K.R., Lee, M-H, Jaffa, A.A., Obeid, L.M. and Luttrell, L.M. (2006) Insulin-like Growth Factors Mediate Heterotrimeric G Protein-dependent ERK1/2 Activation by Transactivating Sphingosine 1-Phosphate Receptors . J. Biol. Chem. 281(42): 31399-31407. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | El-Shewy, H.M., Lee, M-H., Obeid, L.M., Jaffa, A.A. and Luttrell, L.M. (2007) The Insulin-like Growth Factor Type 1 and Insulin-like Growth Factor Type 2/Mannose-6-phosphate Receptors Independently Regulate ERK1/2 Activity in HEK293 Cells. J. Biol. Chem. 282(36): 26150-26157. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Gesty-Palmer, D., Chen, M., Reiter, E., Ahn, S., Nelson, C.D., Wang, S., Eckhardt, A.E., Cowan, C.L., Spurney, R.F., Luttrell, L.M. and Lefkowitz, R.J. (2006) Distinct beta-Arrestin- and G Protein-dependent Pathways for Parathyroid Hormone Receptor-stimulated ERK1/2 Activation. J. Biol. Chem. 281(16): 10856-10864. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | IM Kim, DG Tilley, J Chen, NC Salazar, EJ Whalen, JD Violin, and HA Rockman (2008) Beta-blockers alprenolol and carvedilol stimulate beta-arrestin-mediated EGFR transactivation. Proc Natl Acad Sci 105(38): 14555-60. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Kani, S., Nakayama, E., Yoda, A., Onishi, N., Sougawa, N., Hazaka, Y., Umeda, T., Takeda, K., Ichijo, H., Hamada, Y. and Minami, Y. (2007) Chk2 kinase is required for methylglyoxal-induced G2/M cell-cycle checkpoint arrest: implication of cell-cycle checkpoint regulation in diabetic oxidative stress signaling. Genes Cells. 12(8): 919-928. |
| HEk-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Kara, E., Crepieux, P., Gauthier, C., Martinat, N., Piketty, V., Guillou, F., and Reiter, E. (2006) A Phosphorylation Cluster of Five Serine and Threonine Residues in the C-Terminus of the Follicle-Stimulating Hormone Receptor Is Important for Desensitization But Not for β-Arrestin-Mediated ERK Activation. Mol. Endocrinol. 20(11): p. 3014-3026. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Nelson, C.D., Kovacs, J.J., Nobles, K.N., Whalen, E.J. and Lefkowitz, R.J. (2008) β-Arrestin Scaffolding of Phosphatidylinositol 4-Phosphate 5-Kinase Iα Promotes Agonist-stimulated Sequestration of the {beta}2-Adrenergic Receptor. J. Biol. Chem. 283(30): p. 21093-21101. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Noma, T., Lemaire, A., Prasad, S.V.N., Barki-Harrington, L., Tilley, D.G., Chen, J., Le Corvoisier, P., Violin, J.D., Wei, H., Lefkowitz, R.J. and Rockman, H.A. (2007) β-Arrestin mediated β1-adrenergic receptor transactivation of the EGFR confers cardioprotection. J. Clin. Invest. 117(9): p. 2445-2458. |
| HEk-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Rajagopal, K., Whalen, E.J., Violin, J.D., Stiber, J.A., Rosenberg, P.B., Premont, R.T., Coffman, T.M., Rockman, H.A. and Lefkowitz, R.J. (2006) β-Arrestin2-mediated inotropic effects of the angiotensin II type 1A receptor in isolated cardiac myocytes. PNAS. 103: 16284 - 16289. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Shenoy, S.K., Xiao, K., Venkataramanan, V., Snyder, P.M., Freedman, N.J. and Weissman, A.M. (2008) NEDD4 mediates agonist-dependent ubiquitination, lysosomal targeting and degradation of the beta 2 adrenergic receptor. J. Biol. Chem. 283: 22166 - 22176. |
| HEK-293 | Embryonic Kidney | Human | GeneSilencer siRNA Transfection Reagent | Violin, J.D., DeWire, S.M., Barnes, W.G. and Lefkowitz, R.J. (2006) G Protein-Coupled Receptor Kinase and β-arrestin mediated desensitization of the angiotensin II type 1A receptor elucidated by diacylgylcerol dynamics. J. Biol. Chem. 281: 36411 - 36419. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Bentley, A.M., Normand, G., Hoyt, J. and King, R.W. (2007) Distinct Sequence Elements of Cyclin B1 Promote Localization to Chromatin, Centrosomes, and Kinetochores during Mitosis. Mol. Biol. Cell, Dec 2007; 18: 4847 - 4858. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Cassimeris, L. and Morabito, J. (2004) TOGp, the Human Homolog of XMAP215/Dis1, Is Required for Centrosome Integrity, Spindle Pole Organization, and Bipolar Spindle Assembly. Mol. Biol. Cell 15 (4): 1580-1590. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Chung, J-S., Sato, K., Dougherty, I.I., Cruz, Jr., P.D. and Ariizumi, K. (2007) DC-HIL is a negative regulator of T lymphocyte activation. Blood. 109(10): p. 4320-4327. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Kheifets, V., Bright, R., Inagaki, K., Schechtman, D. and Mochly-Rosen, D. (2006) Protein Kinase C δ}PKC)-Annexin V Interaction: a required step in δ PKC translocation and function. J. Biol. Chem. 281(32): p. 23218-23226. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Layzer, J.M., McCaffrey, A.P., Tanner, A.K., Huang, Zan, Kay, Mark A. and Sullenger, B.A. (2004) In vivo activity of nuclease-resistant siRNAs. RNA 10 (5): p. 766-771. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Pomerening, J.R., Ubersax, J.A. and Ferrell, J.E., Jr. (2008) Rapid Cycling and Precocious Termination of G1 Phase in Cells Expressing CDK1AF. Mol. Biol. Cell, 19: 3426 - 3441. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Shulga, N. and Pastorino, J.G. (2006) Acyl Coenzyme A-binding Protein Augments Bid-induced Mitochondrial Damage and Cell Death by Activating μ-Calpain. J. Biol. Chem. 281(41): 30824-30833. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Witherow, D.S., Garrison, T.R., Miller, W.E. and Robert J. Lefkowitz (2004) {beta}-Arrestin inhibits NF-{kappa}B activity by means of its interaction with the NF-{kappa}B inhibitor I{kappa}B{alpha}. Proc. Natl. Acad. Sci. USA 101: 8603-8607. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Yang, Q-H, Church-Hajduk, R., Ren, J., Newton, M.L. and Du, C. (2003) Omi/HtrA2 catalytic cleavage of inhibitor of apoptosis (IAP) irreversibly inactivates IAPs and facilitates caspase activity in apoptosis. Genes & Dev. 17: 1487 - 1496. |
| HeLa | Cervical Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Zhu, S., Wang, W., Clarke, D.C. and Liu, X. (2007) Activation of Mps1 Promotes Transforming Growth Factor-β-independent Smad Signaling. J. Biol Chem. 282 (25) 18327-18338. |
| HepG2 | Hepatocellular Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Pastorino, J.G. and Shulga, N. (2008) TNFalpha can provoke cleavage and activation of sterol regulatory element binding protein in ethanol exposed cells via a caspase dependent pathway that is cholesterol-insensitive. J. Biol. Chem. 283: 25638 - 25649. |
| HL-60 | Peripheral Blood Promyelocytic Leukemia | Human | GeneSilencer siRNA Transfection Reagent | Smirnova, I.V., Kajstura, M., Sawamura, T. and Goligorsky, M.S. (2004) Asymmetric dimethylarginine upregulates LOX-1 in activated macrophages: role in foam cell formation. AJP: Heart 287(2): p. H782-H790 |
| HMEC-1 | Human Microvascular Endothelial Cells | Human | GeneSilencer siRNA Transfection Reagent | Zhou, X., Stuart, A., Dettin, L.E., Rodriguez, G., Hoel, B. and Gallicano G.I. (2004) Desmoplakin is required for microvascular tube formation in culture. J. Cell Sci. 117: 3129-3140. |
| HPAEC | Pulmonary Artery Endothelium | Human | GeneSilencer siRNA Transfection Reagent | Berdyshev, E.V., Gorshkova, I., Skobeleva, A., Bittman, R., Lu, X., Dudek, S.M., Mirzapoiazova, T., Garcia, J.G.N. and Natarajan, V. (2009) FTY720 inhibits ceramide synthases and upregulates dihydrosphingosine-1-phosphate formation in human lung endothelial cells. J. Biol. Chem. 284 (9): 5467-5477. |
| HT29 | Colon Adenocarcinoma | Human | GeneSilencer siRNA Transfection Reagent | Yao, K., Shida, S., Selvakumaran, M., Zimmerman, R., Simon, E., Schick, J., Haas, N.B. Balke, M., Ross, H., Johnson, S.W. and O"Dwyer, P.J. (2005) Macrophage Migration Inhibitory Factor Is a Determinant of Hypoxia-Induced Apoptosis in Colon Cancer Cell Lines. Clin. Can. Res. 11: 7264-7272. |
| HUT-78 | T Cells | Human | GeneSilencer siRNA Transfection Reagent | Maneechotesuwan, K., Xin, Y., Ito, K., Jazrawi, E., Lee, K-Y., Usmani, O.S., Barnes, P.J. and Adcock, I.M. (2007) Regulation of Th2 Cytokine Genes by p38 MAPK-Mediated Phosphorylation of GATA-3. J. Immunol. 178(4): 2491-2498. |
| JAR | Choriocarcinoma | Human | GeneSilencer siRNA Transfection Reagent | Abboud-Jarrous, G., Atzmon, R., Peretz, T., Palermo, C., Gadea, B.B., Joyce, J.A. and Vlodavsky, I. (2008) Cathepsin L Is Responsible for Processing and Activation of Proheparanase through Multiple Cleavages of a Linker Segment. J. Biol. Chem. 283: 18167 - 18176. |
| Jurkat | T-Cell Lymphoma | Human | GeneSilencer siRNA Transfection Reagent | Nguyen, J.T. and Wells, J.A. (2003) Direct activation of the apoptosis machinery as a mechanism to target cancer cells. Proc. Natl. Acad. Sci. 100: 7533 7538. |
| KATOIII | Gastric Epithelium | Human | GeneSilencer siRNA Transfection Reagent | Nagasako, T., Sugiyama, T., Mizushima, T., Miura, Y., Kato, M. and Asaka, M. (2003) Up-regulated Smad5 Mediates Apoptosis of Gastric Epithelial Cells Induced by Helicobacter pylori Infection. J. Biol. Chem: 278: 4821 - 4825. |
| L/Stab-2 | Fibroblast | Mouse | GeneSilencer siRNA Transfection Reagent | Park, S-Y., Kang, K-B., Thapa, N., Kim, S-Y., Lee, S-J. and Kim, I-S. (2008) Requirement of Adaptor Protein GULP during Stabilin-2-mediated Cell Corpse Engulfment. J. Biol. Chem. 283(16): 10593-10600. |
| LNCaP | Prostate Cancer | Human | GeneSilencer siRNA Transfection Reagent | Takayama, K., Tsutsumi, S., Suzuki, T., Horie-Inoue, K., Ikeda, K., Kaneshiro, K. Fujimura, T., Kumagai, J., Urano, T., Sakaki, Y., Shirahige, K., Sasano, H., Takahashi, S., Kitamura, T., Ouchi, Y., Aburatani, H. and Inoue, S. (2009) Amyloid precursor protein is a primary androgen target gene that promotes prostate cancer growth. Cancer Res 1 69(1): p. 137. |
| M2 | Melanoma | Human | GeneSilencer siRNA Transfection Reagent | Scott, M.G.H., Pierotti, V., Storez, H., Lindberg, E., Thuret, A., Muntaner, O., Labbe-Jullie, C., Pitcher, J.A. and Marullo, S. (2006) Cooperative Regulation of Extracellular Signal-Regulated Kinase Activation and Cell Shape Change by Filamin A and β-Arrestins. Mol. & Cell Biol, 26: 3432-3445. |
| McA-RH 7777 | Hepatoma | Rat | GeneSilencer siRNA Transfection Reagent | Pastorino, J.G. and Shulga, N. (2008) Tumor Necrosis Factor-{alpha} Can Provoke Cleavage and Activation of Sterol Regulatory Element-binding Protein in Ethanol-exposed Cells via a Caspase-dependent Pathway That Is Cholesterol Insensitive. J. Biol. Chem. 283: 25638 - 25649. |
| MCF-7 | Breast Adenocarcinoma | Human | GeneSilencer siRNA Transfection Reagent | Ikeda, K., Ogawa, S., Tsukui, T., Horie-Inoue, K., Ouchi, Y., Kato, S., Muramatsu, M. and Inoue, S. (2004) Protein Phosphatase 5 Is a Negative Regulator of Estrogen Receptor-mediated Transcription. Mol. Endocrinol. 18(5): 1131-1143. |
| MDA MB-231 | Breast Cancer | Human | GeneSilencer siRNA Transfection Reagent | Ge, L., Shenoy, S.K., Lefkowitz, R.J. and DeFea, K.A. (2004) Constitutive protease-activated-receptor-2 mediated migration of MDA MB-231 breast cancer cells requires both beta-arrestin-1 and 2. J. Biol. Chem. 279(53): p. 55419-55424. |
| MDA MB-468 | Breast Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Ge, L., Shenoy, S.K., Lefkowitz, R.J. and DeFea, K.A. (2004) Constitutive protease-activated-receptor-2 mediated migration of MDA MB-231 breast cancer cells requires both beta-arrestin-1 and 2. J. Biol. Chem.279(53): p. 55419-55424. |
| MDA-MB-435 | Melanoma | Human | GeneSilencer siRNA Transfection Reagent | Abboud-Jarrous, G., Atzmon, R., Peretz, T., Palermo, C., Gadea, B.B., Joyce, J.A.and Vlodavsky, I. (2008) Cathepsin L Is Responsible for Processing and Activation of Proheparanase through Multiple Cleavages of a Linker Segment. J. Biol. Chem. 283: 18167 - 18176. |
| MDA-MB-468 | Breast Carcinoma | Human | GeneSilencer siRNA Transfection Reagent | Zoudilova, M., Kumar, P., Ge, L., Wang, P., Bokoch, G. M. and DeFea, K. A. (2007) β-arrestin-dependent regulation of the cofilin pathway downstream of protease-activated receptor-2. J. Biol. Chem. 282: 20634 - 20646. |
| MG63 | Osteosarcoma | Human | GeneSilencer siRNA Transfection Reagent | Ichikawa, T., Horie-Inoue, K., Ikeda, K., Blumberg, B. and Inoue, S. (2007) Vitamin K2 induces phosphorylation of protein kinase A and expression of novel target genes in osteoblastic cells. J Mol Endocrinol 1 39 (4): 239. |
| MKN 28 | Gastric Epithelium | Human | GeneSilencer siRNA Transfection Reagent | Nagasako, T., Sugiyama, T., Mizushima, T., Miura, Y., Kato, M. and Asaka, M. (2003) Up-regulated Smad5 Mediates Apoptosis of Gastric Epithelial Cells Induced by Helicobacter pylori Infection. J. Biol. Chem.: 278: 4821 - 4825. |
| MKN 45 | Gastric Epithelium | Human | GeneSilencer siRNA Transfection Reagent | Nagasako, T., Sugiyama, T., Mizushima, T., Miura, Y., Kato, M. and Asaka, M. (2003) Up-regulated Smad5 Mediates Apoptosis of Gastric Epithelial Cells Induced by Helicobacter pylori Infection. J. Biol. Chem.: 278: 4821 - 4825. |
| MV-4-11 | Biophenotypic B Myelomonocytic Leukemia | Human | GeneSilencer siRNA Transfection Reagent | Yang, X., Liu, L., Sternberg, D., Tang, L., Galinsky, I., DeAngelo, D. and Stone, R. (2005) The FLT3 Internal Tandem Duplication Mutation Prevents Apoptosis in Interleukin-3-Deprived BaF3 Cells Due to Protein Kinase A and Ribosomal S6 Kinase 1-Mediated BAD Phosphorylation at Serine 112. Cancer Res. 65(16): p. 7338-7347. |
| Neuro2a | Neuroblastoma | Mouse | GeneSilencer siRNA Transfection Reagent | Bertling, E., Hotulainen, P., Mattila, P.K., Matilainen, T., Salminen, M., and Lappalainen, P. (2004) Cyclase-associated-protein 1 (CAP1) promotes cofilin-induced actin dynamics in mammalian nonmuscle cells. Mol. Biol. Cell 15 (5): p. 2324-2334. |
| NIH-3T3 | Fibroblast | Mouse | GeneSilencer siRNA Transfection Reagent | Bertling, E., Hotulainen, P., Mattila, P.K., Matilainen, T., Salminen, M., and Lappalainen, P. (2004) Cyclase-associated-protein 1 (CAP1) promotes cofilin-induced actin dynamics in mammalian nonmuscle cells. Mol. Biol. Cell 15 (5): 2324-2334. |
| NIH-3T3 | Fibroblast | Mouse | GeneSilencer siRNA Transfection Reagent | Hotulainen, P., Paunola, E., Vartiainen, M.K. and Lappalainen, P. (2005) Actin-depolymerizing Factor and Cofilin-1 Play Overlapping Roles in Promoting Rapid F-Actin Depolymerization in Mammalian Nonmuscle Cells. Mol. Biol. Cell. 16(2): p. 649-664. |
| NIH-3T3 | Fibroblast | Mouse | GeneSilencer siRNA Transfection Reagent | Nomachi, A., Nishita, M., Inaba, D., Enomoto, M., Hamasaki, M. and Minami, Y. (2008) Receptor Tyrosine Kinase Ror2 Mediates Wnt5a-induced Polarized Cell Migration by Activating c-Jun N-terminal Kinase via Actin-binding Protein Filamin A. J. Biol. Chem. 283(41): 27973-27981. |
| OK | Kidney | Opossum | GeneSilencer siRNA Transfection Reagent | Khundmiri, S.J., Ameen, M., Delamere, N.A. and Lederer, E.D. (2008) PTH-mediated regulation of Na+ -K+ -ATPase requires Src kinase-dependent ERK Phosphorylation. Am J Physiol Renal Physiol. 295(2): F426-F437. |
| OK | Kidney | Opossum | GeneSilencer siRNA Transfection Reagent | Khundmiri, S.J., Dean, W.L., McLeish, K.R. and Lederer, E.D. (2005) Parathyroid Hormone-mediated Regulation of Na+-K+-ATPase Requires ERK-dependent Translocation of Protein Kinase C{alpha}. J. Biol. Chem. 280(10): p. 8705-8713. |
| PC-12 | Pheochromocytoma | Rat | GeneSilencer siRNA Transfection Reagent | de Barry, J., Janoshazi, A., Luc Dupont, J., Procksch, O., Chasserot-Golaz, S., Jeromin, A. and Vitale, N. (2006) Functional Implication of Neuronal Calcium Sensor-1 and Phosphoinositol 4-Kinase-β Interaction in Regulated Exocytosis of PC12 Cells. J. Biol Chem. 281(26): 18098-18111. |
| Primary | Cortical Neurons | Mouse | GeneSilencer siRNA Transfection Reagent | Aarts, M., Lihara, K., Wei, W-L, Xiong, Z-G, Arundine, M., Cerwinski, W., MacDonald, J.F., Tymianski, M. (2003) A Key Role for TRPM7 Channels in Anoxic Neuronal Death. Cell 115: 863. |
| Primary | Cortical Neurons | Mouse | GeneSilencer siRNA Transfection Reagent | Aleyasin, H., Cregan, S.P., Iyirhiaro, G., OHare, M.J., Callaghan, S.M., Slack, R.S. and Park, D.S. (2004) Nuclear Factor-B Modulates the p53 Response in Neurons Exposed to DNA Damage. J. Neurosci. 24 (12): 2963-2973. |
| Primary | Hippocampal Neurons | Rat | GeneSilencer siRNA Transfection Reagent | Amaral, M.D. and Pozzo-Miller, L. (2007) TRPC3 Channels Are Necessary for Brain-Derived Neurotrophic Factor to Activate a Nonselective Cationic Current and to Induce Dendritic Spine Formation. J. Neurosci. 27(19): p. 5179-5189. |
| Primary | Macrophage | Human | GeneSilencer siRNA Transfection Reagent | Asmis, R., Wang, Y., Xu, L., Kisgati, M., Begley, J.G. and驴Mieyal, J.J. (2005) A novel thiol oxidation-based mechanism for adriamycin-induced cell injury in human macrophages. FASEB J. published 13 September 2005, 10.1096/fj.04-2991fje. |
| Primary | Pulmonary Artery Endothelium | Human | GeneSilencer siRNA Transfection Reagent | Birukova, A.A., Chatchavalvanich, S., Rios, A., Kawkitinarong, K., Garcia, J.G.N. and Birukov, K.G. (2006) Differential Regulation of Pulmonary Endothelial Monolayer Integrity by Varying Degrees of Cyclic Stretch. Am. J. Pathol. 168: 1749 - 1761. |
| Primary | Cortical Neurons | Mouse | GeneSilencer siRNA Transfection Reagent | Burkhalter, J., Fiumelli, H., Erickson, J.D. and Martin, J-L. (2007) A critical role for system a amino acid transport in the regulation of dendritic development by BDNF. J Biol Chem 282: 5152 - 5159. |
| Primary | Dendritic Cells (Bone Marrow) | Mouse | GeneSilencer siRNA Transfection Reagent | Chung, J-S, Sato, K., Dougherty, I.I., Cruz, Jr., P.D. and Ariizumi, K. (2007) DC-HIL is a negative regulator of T lymphocyte activation. Blood. 109(10): p. 4320-4327. |
| Primary | Cortical Neurons | Mouse | GeneSilencer siRNA Transfection Reagent | Cui, H., Hayashi, A., Sun, H-S, Belmares, M.P., Cobey, C., Phan, T., Schweizer, J., Salter, M.W., Wang, Y.T., Tasker, R.A., Garman, D., Rabinowitz, J., Lu, P.S. and Tymianski, M. (2007) PDZ Protein Interactions Underlying NMDA Receptor-Mediated Excitotoxicity and Neuroprotection by PSD-95 Inhibitors. J. Neurosci. 27(37): 9901-9915. |
| Primary | Coronary Artery Endothelial | Human HCAEC | GeneSilencer siRNA Transfection Reagent | Dandapat, A., Hu, C., Sun, L. and Mehta, J.L. (2007) Small Concentrations of oxLDL Induce Capillary Tube Formation From Endothelial Cells via LOX-1 Dependent Redox-Sensitive Pathway. Arterioscler Thromb Vasc Biol. |
| Primary | Peritoneal Macrophage | Mouse | GeneSilencer siRNA Transfection Reagent | de Beer, M.C., Zhao, Z., Webb, N.R., van der Westhuyzen, D.R. and de Villiers, W.J.S. (2003) Lack of a direct role for macrosialin in oxidized LDL metabolism J. Lipid Res. 44: 674 - 685. |
| Primary | Dorsal Root Ganglion Neurons | Mouse | GeneSilencer siRNA Transfection Reagent | Haruhisa Higuchi, Toshihide Yamashita, Hideki Yoshikawa, and Masaya Tohyama (2003) Functional inhibition of the p75 receptor using a small interfering RNA. Biochem. & Biophys. Res. Comm. 301: 804809. |
| Primary | Retinal Ganglion | Rat | GeneSilencer siRNA Transfection Reagent | Hayashi, H., Campenot, R.B., Vance, D.E. and Vance, J.E. (2007) Apolipoprotein E-Containing Lipoproteins Protect Neurons from Apoptosis via a Signaling Pathway Involving Low-Density Lipoprotein Receptor-Related Protein-1. J. Neurosci. 27(8): 1933-1941. |
| Primary | Dorsal Root Ganglion | Rat | GeneSilencer siRNA Transfection Reagent | Hengst, U. Cox, L.J., Macosko, E.Z. and Jaffrey, S.R. (2006) Functional and Selective RNA Interference in Developing Axons and Growth Cones. J. Neurosci. 26(21): 5727-5732. |
| Primary | Schwann Cells | Mouse | GeneSilencer siRNA Transfection Reagent | Higuchi, H., Yamashita, T., Yoshikawa, H. and Tohyama, M. (2003) Functional inhibition of the p75 receptor using a small interfering RNA. Biochem. & Biophys. Res. Comm. 301: 804809. |
| Primary | Cardiac Fibroblast | Mouse | GeneSilencer siRNA Transfection Reagent | Hu, C., Dandapat, A., Sun, L., Khan, J.A., Liu, Y., Hermonat, P.L. and Mehta, J.L. (2008) Regulation of TGFbeta 1-mediated collagen formation by LOX-1: Studies based on forced over-expression of TGFbeta 1 in wild-type and LOX-1 knockout mice cardiac fibroblasts. J. Biol. Chem. 283 (16) 10226-10231. |
| Primary | Cardiac Myocytes (Neonatal) | Rat | GeneSilencer siRNA Transfection Reagent | Juhaszova, M., Zorov, D.B., Kim, S-H, Pepe, S., Fu, O., Fishbein, K.W., Ziman, B.D., Wang, S., Ytrehus, K., Antos, C.L., Olson, E.N. and Sollott, S.J. (2004) Glycogen synthase kinase-3{beta} mediates convergence of protection signaling to inhibit the mitochondrial permeability transition pore. J. Clin. Invest. 113(11): p. 1535-1549. |
| Primary | Lung Microvascular Endothelial Cells | Human | GeneSilencer siRNA Transfection Reagent | Kolosova, I.A., Ma, S-F, Adyshev, D.M., Wang, P., Ohba, M., Natarajan, V., Garcia, J.G.N. and Verin, A.D. (2004) Role of CPI-17 in the regulation of endothelial cytoskeleton AJP: Lung. 287(5): L970. |
| Primary | Aortic Muscle Cells | Rat | GeneSilencer siRNA Transfection Reagent | Lake, A.C. & Castellot, J.J. (2003) CCN5 modulates the antiproliferative effect of heparin and regulates cell motility in vascular smooth muscle cells. Cell Comm. & Signaling 1: 5. |
| Primary | Dendritic Cells | Mouse | GeneSilencer siRNA Transfection Reagent | Li, M., Qian, H., Ichim, T.E., Ge, W-W, Popov, I.A., Rycerz, K., Neu, J., White, D., Zhong, R., Min, W-P. (2004) Induction of RNA Interference in Dendritic Cells. Immunologic Research 30 (2) 215-230. |
| Primary | Dendritic Cells | Mouse | GeneSilencer siRNA Transfection Reagent | Li, M., Zhang, X., Zheng, X., Lian, D., Zhang, Z-X., Ge, W., Yang, J., Vladau, C., Suzuki, M., Chen, D., Zhong, R., Garcia, B., Jevnikar, A.M. and Min, W-P (2007) Immune Modulation and Tolerance Induction by RelB-Silenced Dendritic Cells through RNA Interference. J. Immunol. 178(9): p. 5480-5487. |
| Primary | Dendritic Cells | Mouse | GeneSilencer siRNA Transfection Reagent | Liu, G., Ng, H., Akasaki, Y., Yuan, X., Ehtesham, M., Yin, D., Black , K.L. and Yu, J.S. (2004) Small interference RNA modulation of IL-10 in human monocyte-derived dendritic cells enhances the Th1 response. Eur. J. Immunol. 34: 1680-1687. |
| Primary | Cerebellar Neurons | Rat | GeneSilencer siRNA Transfection Reagent | Numakawa, T., Nakayama, H., Suzuki, S., Kubo, T., Nara, F., Numakawa, Y., Yokomaku, D., Araki, T., Ishimoto, T., Ogura, A. and Taguchi, T. (2003) Nerve growth factor-induced glutamate release is via p75 receptor, ceramide and Ca2+ from ryanodine receptor in developing cerebellar neurons. J. Biol. Chem: 278: 41259-41269. |
| Primary | Osteoblast | Mouse | GeneSilencer siRNA Transfection Reagent | Ohyama, Y., Nifuji, A., Maeda, Y., Amagasa, T. and Noda, M. (2004) Spaciotemporal Association and Bone Morphogenetic Protein Regulation of Sclerostin and Osterix Expression during Embryonic Osteogenesis. Endocrinology. 145(10): p. 4685-4692. |
| Primary | HMVEC | Human | GeneSilencer siRNA Transfection Reagent | Radu Stefanescu, Dustin Bassett, Rozbeh Modarresi, Francisco Santiago, Mohamad Fakruddin, and Jeffrey Laurence (2008) Synergistic interactions between interferon-{gamma} and TRAIL modulate c-FLIP in endothelial cells, mediating their lineage-specific sensitivity to thrombotic thrombocytopenic purpura plasma-associated apoptosis. Blood 112: 340 - 349. |
| Primary | Cardiac Myocytes | Feline | GeneSilencer siRNA Transfection Reagent | Ramabadran, R. S., Chancey, A., Vallejo, J.G., Barger, P.M., Sivasubramanian, N. and Mann, D.L. (2008) Targeted Gene Silencing of Tumor Necrosis Factor Attenuates the Negative Inotropic Effects of Lipopolysaccharide in Isolated Contracting Cardiac Myocytes. Tex Heart Inst J. 35(1): 16-21. |
| Primary | T-Cells (CD3+) | Human | GeneSilencer siRNA Transfection Reagent | Samten, B., Howard, S.T., Weis, S.E., Wu, S., Shams, H., Townsend, J.C., Safi, H. and Barnes, P.F. (2005) Cyclic AMP Response Element-Binding Protein Positively Regulates Production of IFN-{gamma} by T Cells in Response to a Microbial Pathogen. J. Immunol. 174(10): p. 6357-6363. |
| Primary | Cortical Neurons | Mouse | GeneSilencer siRNA Transfection Reagent | Sato, S., Xu, J., Okuyama, S., Martinez, LB., Walsh, S.M, Jacobsen, M.T, Swan, R.J., Schlautman, J.D., Ciborowski, P. and Ikezu, T. (2008) Spatial learning impairment, enhanced CDK5/p35 activity, and downregulation of NMDA receptor expression in transgenic mice expressing tau-tubulin kinase 1. J Neurosci 28(53): 14511. |
| Primary | Dendritic Cells | Human | GeneSilencer siRNA Transfection Reagent | Smith, A.L., Ganesh, L., Leung, K., Jongstra-Bilen, J., Jongstra, J. and Nabel, G.J. (2007) Leukocyte-specific protein 1 interacts with DC-SIGN and mediates transport of HIV to the proteasome in dendritic cells. J. Exp. Med. 204(2): p. 421-430. |
| Primary | Cortical Neurons | Mouse | GeneSilencer siRNA Transfection Reagent | Supnet, C., Grant, J., Kong, H., Westaway, D. and Mayne, M. (2006) Amyloid β-(1-42) Increases Ryanodine Receptor-3 Expression and Function in TgCRND8 Mice. J. Biol. Chem. 281: 38440 - 38447. |
| Primary | Aveolar Type II Cells | Mouse | GeneSilencer siRNA Transfection Reagent | Ueno, T., Linder, S., Na, C-L, Rice, W.R., Johansson, J., and Weaver, T.E. (2004) Processing of Pulmonary Surfactant Protein B by Napsin and Cathepsin H. J. Biol. Chem. 279: 16178-16184. |
| Primary | Macrophage | Mouse | GeneSilencer siRNA Transfection Reagent | Wang, Y., Chen, T., Han, C., He, D., Liu, H., An, H., Cai, Z. and Cao, X. (2007) Lysosome-associated small Rab GTPase Rab7b negatively regulates TLR4 signaling in macrophages by promoting lysosomal degradation of TLR4. Blood 110: 962 - 971. |
| Primary | Dorsal Root Ganglion | Rat | GeneSilencer siRNA Transfection Reagent | Wu, K.Y., Hengst, U., Cox, L.J., Macosko, E.Z., Jeromin, A., Urquhart, E.R., and Jaffrey, S.R. (2005) Local translation of RhoA regulates growth cone collapse. Nature 436: 1020-1024. |
| Primary | Lacrimal Gland Acinar Cells | Rabbit | GeneSilencer siRNA Transfection Reagent | Xie, J., Chiang, L., Contreras, J., Wu, K., Garner, J.A., Medina-Kauwe, L. and Hamm-Alvarez, S.F. (2006) Novel Fiber-Dependent Entry Mechanism for Adenovirus Serotype 5 in Lacrimal Acini. J. Virol. 80(23): 11833 - 11851. |
| Primary | Bone Marrow | Mouse | GeneSilencer siRNA Transfection Reagent | Yang, R., Cai, Z., Zhang, Y., Yutzy IV, W.H., Roby, K.F. and Roden, R.B.S. (2006) CD80 in Immune Suppression by Mouse Ovarian Carcinoma驴Associated Gr-1+CD11b+ Myeloid Cells. Cancer Res 66 (13): 6807 - 6815. |
| Primary | Dendritic Cells | Mouse | GeneSilencer siRNA Transfection Reagent | Zheng, X., Koropatnick, J., Li, M., Zhang, X., Ling, F., Ren, X., Hao, X., Sun, H., Vladau, C., Franek, J.A., Feng, B., Urquhart, B.L., Zhong, R., Freeman, D.J., Garcia, B. and Min, W-P. (2006) Reinstalling Antitumor Immunity by Inhibiting Tumor-Derived Immunosuppressive Molecule IDO through RNA Interference. J. Immunol. 177(8): 5639-5646. |
| Primary | Pulmonary Artery Endothelium | Human | GeneSilencer siRNA Transfection Reagent | Zhuowei Li, Xhevahire Hyseni, Jacqueline D. Carter, Joleen M. Soukup, Lisa A. Dailey, and Yuh-Chin T. Huang (2006) Pollutant particles enhanced H2O2 production from NAD(P)H oxidase and mitochondria in human pulmonary artery endothelial cells. Am J Physiol Cell Physiol 291: C357 - C365. |
| Primary HPAEC | Pulmonary Artery Endothelium | Human | GeneSilencer siRNA Transfection Reagent | Rentsendorj, O., Mirzapoiazova, T., Adyshev, D., Servinsky, L.E., Renne, T., Verin, A.D. and Pearse, D.B. (2008) Role of vasodilator-stimulated phosphoprotein in cGMP-mediated protection of human pulmonary artery endothelial barrier function. Am J Physiol Lung Cell Mol Physiol. 294(4): L686 -L697. |
| RAW 264.7 | Macrophage | Mouse | GeneSilencer siRNA Transfection Reagent | de Beer, M.C., Zhao, Z., Webb, N.R., van der Westhuyzen, D.R. and de Villiers, W.J.S. (2003) Lack of a direct role for macrosialin in oxidized LDL metabolism. J. Lipid Res. 44: 674 - 685. |
| RAW 264.7 | Macrophage | Mouse | GeneSilencer siRNA Transfection Reagent | Liu, X., Yao, M., Li, N., Wang, C., Zheng, Y. and Cao, X. (2008) CaMKII promotes TLR-triggered proinflammatory cytokine and type I interferon production by directly binding and activating TAK1 and IRF3 in macrophages. Blood. 112(13): 4961-4970. |
| Renca | Renal Cell Carcinoma | Mouse | GeneSilencer siRNA Transfection Reagent | Ogushi, T., Takahashi, S., Takeuchi, T., Urano, T., Horie-Inoue, K., Kumagai, J., Kitamura, T., Ouchi, Y., Muramatsu, M. and Inoue, S. (2005) Estrogen Receptor-Binding Fragment-Associated Antigen 9 Is a Tumor-Promoting and Prognostic Factor for Renal Cell Carcinoma. Cancer Res. 65(9): p. 3700-3706. |
| SaOs2 | Osteosarcoma | Human | GeneSilencer siRNA Transfection Reagent | Chu, F.,Chou, P.M., Zheng, X., Mirkin, B.L. and Rebbaa, A.驴(2005)驴Control of Multidrug Resistance Gene mdr1 and Cancer Resistance to Chemotherapy by the Longevity Gene sirt1. Cancer Res.65 (22): p. 10183-10187. |
| SaOS2 | Osteosarcoma | Human | GeneSilencer siRNA Transfection Reagent | Zheng, X., Chu, F., Chou, P.M., Gallati, C., Dier, U., Mirkin, B.L., Mousa, S.A. and Rebbaa, A. (2009) Cathepsin L inhibition suppresses drug resistance in vitro and in vivo: a putative mechanism. Am J Physiol Cell Physiol. 296(1): C65-C74. |
| Saos-2 | Osteosarcoma | Human | GeneSilencer siRNA Transfection Reagent | Zheng, X., Chou, P.M., Mirkin, B.L. and Rebbaa, A. (2004) Senescence-initiated Reversal of Drug Resistance: Specific Role of Cathepsin L. Cancer Res. 64: 1773 - 1780. |
| SCCH196 | Sarcoma | Human | GeneSilencer siRNA Transfection Reagent | Takahashi, A., Higashino, F., Aoyagi, M., Yoshida, K., Itoh, M., Kyo, S., Ohno, T., Taira, T., Ariga, H., Nakajima, K., Hatta, M., Kobayashi, M., Sano, H., Kohgo, T. and Shindoh, M., (2003) EWS/ETS Fusions Activate Telomerase in Ewings Tumors. Cancer Res. 63: 8338-8344. |
| SNB19 | Glioblastoma | Human | GeneSilencer siRNA Transfection Reagent | Song, S.W., Fuller, G.N., Zheng, H. and Zhang, W. (2005) Inactivation of the Invasion Inhibitory Gene IIp45 by Alternative Splicing in Gliomas. Cancer Res. 65(9): p. 3562-3567. |
| SW480 | Colon Adenocarcinoma | Human | GeneSilencer siRNA Transfection Reagent | Zhu, S., Wang, W., Clarke, D.C. and Liu, X. (2007) Activation of Mps1 Promotes Transforming Growth Factor-β-independent Smad Signaling. J. Biol. Chem. 282(25): 18327-18338. |
| U20S | Osteosarcoma | Human | GeneSilencer siRNA Transfection Reagent | Hotulainen P. and Lappalainen, P. (2006) Stress fibers are generated by two distinct actin assembly mechanisms in motile cells J. Cell Biol. 173(3): 383-394. |
蚂蚁淘电商平台
ebiomall.com
ebiomall.com
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蚂蚁淘（www.ebiomall.cn）是中国大陆目前唯一的生物医疗科研用品B2B跨境交易平台，
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淘搜索全球供应信息，找到合适的产品后在蚂蚁淘下单，然后蚂蚁淘的海外买手进行跨境采购、
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2021-07-18
胸膜粘连术是治疗恶性胸水的主要方法之一，但如果肺已受恶性肿瘤侵犯导致肺萎陷不能复张，则适宜留置胸腔导管。因为目前还没有很好的方法能在胸水穿刺引流前诊断萎陷肺，为确定治疗方案，患者往往需要经受多次胸腔穿刺。 超声诊断中有很多分析组织运动的模式，如运动模式（M Mode），能定量组织移位，而更高级的弹性图像通过压力的表现形式能定量组织变形。 为 查看更多
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2017-04-06
医流商城提供净信手持式超声波细胞粉碎机UP-400SLED显示自动和手动（脚踏）两种工作模式报价、参数、图片、特点、咨询等有效信息,100%正品保证,是您网上购买净信手持式超声波细胞粉碎机UP-400SLED显示自动和手动（脚踏）两种工作模式的放心平台 查看更多
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2021-08-28
楚业生物科技（上海）有限公司在发布的中型均质机、高压均质机、细胞破碎仪、均质器、NanoFast NF-60供应信息，浏览与中型均质机、高压均质机、细胞破碎仪、均质器、NanoFast NF-60相关的产品或在搜索更多与中型均质机、高压均质机、细胞破碎仪、均质器、NanoFast NF-60相关的内容。 查看更多
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2021-08-26
功率：150W
随机变幅杆：Φ6
可选配变幅杆：Φ3
破碎容量：10-100ml 查看更多
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2021-08-19
医流商城提供洁美液晶超声波清洗器KS-7200DE参数、价格、图片、功能特色、咨询等有效信息，全网价最低，100%正品保证，是您网上购买洁美液晶超声波清洗器KS-7200DE的放心平台。 查看更多
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成都昱强科技有限公司在发布的scientz-950E超声波细胞粉碎机供应信息，浏览与scientz-950E超声波细胞粉碎机相关的产品或在搜索更多与scientz-950E超声波细胞粉碎机相关的内容。 查看更多
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2021-07-17
Constant高压细胞破碎仪 ConstantSystems是一家专业生产高压细胞破碎仪的英国公司，是ScoreGroupPLC集团成员。ConstantSystems一直致力于高压破碎仪的开发和生产，为用户提供优质和性能稳定的破碎仪。通过其全球的营销网络，产品销往北美、欧洲、中东和亚洲等世界各地，为全球制药和生物技术公司、大学和研究机构提供性能卓越的高压破碎仪和良好的服务。ConstantSystems高压细胞破 查看更多
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2021-08-12
1.功率：150W，240Vl
2.最大处理量：200ul — 100mll
3.自动调谐正存储器(AT/M)——提供全自动运行，存储每一循环结束时的频率
4. 数显参数可精确输入，很容易设置
查看更多
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2021-08-18
宁波新芝生物科技股份有限公司在发布的超声波细胞破碎仪JY88-IIN 、JY96-IIN供应信息，浏览与超声波细胞破碎仪JY88-IIN 、JY96-IIN相关的产品或在搜索更多与超声波细胞破碎仪JY88-IIN 、JY96-IIN相关的内容。 查看更多
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2021-09-03
超声波细胞粉碎机是一种利用强超声在液体中产生空化效应，对物质进行超声处理的多功能、多用途的仪器，能用于动植物组织、细胞、细菌、芽胞菌种的破碎，同时可用来乳化、分离、分散、匀化、提取、脱气、清洗及加速化学反应等等。该机广泛应用于生物化学、微生物学、药物化学、表面化学、物理学、动物学、农学、医学、制药等领域教学、科研、生产。 ... 查看更多
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2017-02-17
图示 JY99-IIDN超声波细胞粉碎机 1、材料与设备 黄精：购于沈阳中街新药特药;循环水多用真空泵;HH一6数显恒温水浴锅;光栅分光光度计：山东高密彩虹分析仪器有限公司;电热鼓风恒温干燥箱;JY99-IIDN超声波细胞粉碎机：宁波新芝生物科技股份有限公司。 2、实验方 查看更多
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2017-01-19
产品概述： 本仪器是一种利用强超声在液体中产生空化效应，对物质进行超声处理的多功能、多用途的仪器，能用于多种动植物细胞、病毒细胞的破碎，同时可用来乳化、分离、匀化、提取、消泡、清洗及加速化学反应等等。被广泛应用于生物化学、微生物学、药物化学、表面化学、物理学、动物学等领域。手持式超声波破碎仪外观美，性能可靠。仪器采用大屏幕显示，中央微机集中控制，超声时间，功率任意设定，样品温度检测显示、频率微机跟踪、故障自动报警。所有功能都显示在液... 查看更多
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商品咨询
人民医院医疗设备招标公告123
未成年QI062017-11-01
由信号发生器来产生一个特定频率的信号，这个特定频率就是换能器的频率，一般应用在超声波设备中的超声波频率为20KHz、25KHz、28KHz、33KHz、40KHz、60KHz。
换能器组件
换能器组件主要由换能器和变幅杆组成。
隔音箱
可以有效地的降低工作过程中的所发出的噪音，保持实验室安静。
超声波细胞破碎仪在我国的行业推广已进入成熟阶段，而应用仍不够普及!该仪器(设备)应用范围非常广泛，这是其它仪器设备所不能比拟的。也正因如此，该仪器(设备)的市场潜力很大，所以生产厂家也日趋增多，这也同时造成了超声清洗行业及市场的相对混乱，可以用八个字来形容“鱼龙混杂，良莠不齐”!把超声波细胞破碎仪进行分类。
换能器组件
换能器组件主要由换能器和变幅杆组成。
隔音箱
可以有效地的降低工作过程中的所发出的噪音，保持实验室安静。
超声波细胞破碎仪在我国的行业推广已进入成熟阶段，而应用仍不够普及!该仪器(设备)应用范围非常广泛，这是其它仪器设备所不能比拟的。也正因如此，该仪器(设备)的市场潜力很大，所以生产厂家也日趋增多，这也同时造成了超声清洗行业及市场的相对混乱，可以用八个字来形容“鱼龙混杂，良莠不齐”!把超声波细胞破碎仪进行分类。
【资源】(分享学习)超声波破碎细胞的原理及超声细胞破碎仪的用途 ...123
lrb20012021-08-23
超声波是物质介质中的一种弹性机械波，它是一种波动形式，因此它可以用于探测人体的生理及病理信息，既诊断超声。同时，它又是一种能量形式，当达到一定剂量的超声在生物体内传播时，通过它们之间的相互作用.超声波是物质介质中的一种弹性机械波，它是一种波动形式，因此它可以用于探测人体的生理及病理信息，既诊断超声。同时，它又是一种能量形式，当达到一定剂量的超声在生物体内传播时，通过它们之间的相互作用，能引起生物体的功能和结构发生变化，即超声生物效应。超声对细胞的作用主要有热效应，空化效应和机械效应。热效应是当超声在介质中传播时，摩擦力阻碍了由超声引起的分子震动，使部分能量转化为局部高热（42-43℃），因为正常组织的临界致死温度为45.7℃，而肿瘤组织比正常组织敏感性高，故在此温度下肿瘤细胞的代谢发生障碍，DNA、RNA、蛋白质合成受到影响，从而杀伤癌细胞而正常组织不受影响。空化效应是在超声照射下，生物体内形成空泡，随着空泡震动和其猛烈的聚爆而产生出机械剪切压力和动荡，使肿瘤出血、组织瓦解以致坏死。另外，空化泡破裂时产生瞬时高温（约5000℃）、高压（可达500×104Pa），可使水蒸气热解离产生.OH自由基和.H原子，由.OH自由基和.H原子引起的氧化还原反应可导致多聚物降解、酶失活、脂质过氧化和细胞杀伤。机械效应是超声的原发效应，超声波在传播过程中介质质点交替地压缩与伸张构成了压力变化，引起细胞结构损伤。杀伤作用的强弱与超声的频率和强度密切相关。
超声波细胞破碎仪的原理并不是太神秘、太复杂。简单说就是将电能通过换能器转换为声能，这种能量通过液体介质(如水)而变成一个个密集的小气泡，这些小气泡迅速炸裂，产生的象小***一样的能量，从而起到破碎细胞等物质的作用(俗称“空化效应”)。
超声波细胞粉碎机是利用一种超声波在液体中产生空化效应的多功能、多用途的仪器；它能用于各种动植物细胞、病毒细胞、细菌及组织的破碎，也可用于各类无机物质的破碎重组，同时可用来乳化、分离、匀化、提取、消泡清洗及加速化学反应等。超声波细胞粉碎机有广泛的用途，如:
1、超声波提取生物纳米（超声波化学合成法）
超声波化学反应中，起关键作用的是声波的空化效应，在超声波的辐照过程中，在液体里将发生空化气泡的形成，长大和崩灭，当空化气泡崩灭时产生一个覆盖着的强压力脉冲，产生许多独特的性质，例如产生高达5000K的高温，大于200Mpa的压力，这就是超声波化学合成的能量来源，利用这些能量能在一些特殊粉末表面合成出纳米粒子。
2、超声波制药（1）注射用医药物质的分散——将磷脂类与胆固醇混合用适当方法与药物混合在水溶液中，经超声分散，可以得到更小粒子供静脉注射。
（2）草药提取——利用超声分散破坏植物组织，加速溶剂穿透组织作用，提高中草药有效成分提取率。如金鸡纳树皮中全部生物碱用一般方法侵出需5小时以上，采用超声分散只要半小时即可完成。
（3）制备混悬剂——在超声空化和强烈搅拌下，将一种固体药物分散在含有表面活性剂的水溶液中，可以形成1um左右口服或静脉注射混悬剂。例“静注喜树碱混悬剂”“肝脏造影剂”、“硫酸钡混悬剂”。
（4）制备疫苗——将细胞或病菌借助于超声分散将其杀死以后，再用适当方法制成疫苗。
3、超声波对化妆品的分散
为了更进一步提取药物精华和粒子微细化，并节约生产成本，达到分散、乳化效果，使化妆品更深入渗透到肌肤里层，让肌肤很好的吸收，发挥药物的效力和作用，采用超声波乳化可达到非常理想的效果。采用超声分散，则不需要使用乳化剂，就能使蜡及石蜡乳化、化妆水等油的微粒子分散。石腊在水中分散的粒子直径可达1um以下。
4、超声波对酒的醇化—催陈技术
一瓶美酒以它的酒味醇厚，绵软柔和、芳香浓郁为人青睐，人们常用陈年老酒来形容酒的珍贵，一瓶上世纪的陈酒，标价几万元，其价格的含义在于时间的存放上。酒的主要控制因素是化学变化即酸的形成，并进一步酯化，酯参与乙醇和水的缔合。刚出厂的酒含有戍醇，有辛辣味，这种气味要经过很长时间才能化解，这个缓慢变化称酒的醇化。用功率1.6KW，频率17.5-22KHZ的超声波处理5-10min，可使酒的老熟时间缩短1/3到1/2。
因此超声波细胞粉碎机可以并且已经被广泛用于生物化学、微生物学药理学、物理学、动物学、农学、医学、制药等领域的教学、科研、生产。
超声波细胞破碎仪的原理并不是太神秘、太复杂。简单说就是将电能通过换能器转换为声能，这种能量通过液体介质(如水)而变成一个个密集的小气泡，这些小气泡迅速炸裂，产生的象小***一样的能量，从而起到破碎细胞等物质的作用(俗称“空化效应”)。
超声波细胞粉碎机是利用一种超声波在液体中产生空化效应的多功能、多用途的仪器；它能用于各种动植物细胞、病毒细胞、细菌及组织的破碎，也可用于各类无机物质的破碎重组，同时可用来乳化、分离、匀化、提取、消泡清洗及加速化学反应等。超声波细胞粉碎机有广泛的用途，如:
1、超声波提取生物纳米（超声波化学合成法）
超声波化学反应中，起关键作用的是声波的空化效应，在超声波的辐照过程中，在液体里将发生空化气泡的形成，长大和崩灭，当空化气泡崩灭时产生一个覆盖着的强压力脉冲，产生许多独特的性质，例如产生高达5000K的高温，大于200Mpa的压力，这就是超声波化学合成的能量来源，利用这些能量能在一些特殊粉末表面合成出纳米粒子。
2、超声波制药（1）注射用医药物质的分散——将磷脂类与胆固醇混合用适当方法与药物混合在水溶液中，经超声分散，可以得到更小粒子供静脉注射。
（2）草药提取——利用超声分散破坏植物组织，加速溶剂穿透组织作用，提高中草药有效成分提取率。如金鸡纳树皮中全部生物碱用一般方法侵出需5小时以上，采用超声分散只要半小时即可完成。
（3）制备混悬剂——在超声空化和强烈搅拌下，将一种固体药物分散在含有表面活性剂的水溶液中，可以形成1um左右口服或静脉注射混悬剂。例“静注喜树碱混悬剂”“肝脏造影剂”、“硫酸钡混悬剂”。
（4）制备疫苗——将细胞或病菌借助于超声分散将其杀死以后，再用适当方法制成疫苗。
3、超声波对化妆品的分散
为了更进一步提取药物精华和粒子微细化，并节约生产成本，达到分散、乳化效果，使化妆品更深入渗透到肌肤里层，让肌肤很好的吸收，发挥药物的效力和作用，采用超声波乳化可达到非常理想的效果。采用超声分散，则不需要使用乳化剂，就能使蜡及石蜡乳化、化妆水等油的微粒子分散。石腊在水中分散的粒子直径可达1um以下。
4、超声波对酒的醇化—催陈技术
一瓶美酒以它的酒味醇厚，绵软柔和、芳香浓郁为人青睐，人们常用陈年老酒来形容酒的珍贵，一瓶上世纪的陈酒，标价几万元，其价格的含义在于时间的存放上。酒的主要控制因素是化学变化即酸的形成，并进一步酯化，酯参与乙醇和水的缔合。刚出厂的酒含有戍醇，有辛辣味，这种气味要经过很长时间才能化解，这个缓慢变化称酒的醇化。用功率1.6KW，频率17.5-22KHZ的超声波处理5-10min，可使酒的老熟时间缩短1/3到1/2。
因此超声波细胞粉碎机可以并且已经被广泛用于生物化学、微生物学药理学、物理学、动物学、农学、医学、制药等领域的教学、科研、生产。
超声细胞破碎仪探头有疙瘩怎么处理123
聚完医药8I2021-08-07
清洗掉就可以了
【求助】超声破碎仪能使药物溶解吗? 细胞技术讨论版论坛123
dululu2021-08-14
挥发油+DMSO用超声破碎仪能溶解？？？？？？？？
溶解后加培养液稀释不会出现悬浮浑浊吗？？？？？？
溶解后加培养液稀释不会出现悬浮浑浊吗？？？？？？
从文自传的书评 (33) Douban123
2017-10-21
工作频率范围：20～25KHz。频率自动跟踪。
可储存十套常规程序数据和一套组合程序，工作方式有定时和计数两种。
组合程序最多可以由十套常规程序组成，可选择循环或不循环工作模式。
超温保护及报警功能。
超声波输出强度自动限定功能。
定时方式工作时间定时：0～99小时59分59秒。
计数方式超声工作次数：0～9999次。
超声时间范围：0～99小时59分59秒。
间隙时间范围：0～99小时59分59秒。间隙时间=0为超声连续工作。
温度控制精度：±1℃。
工作电压：VAC100-240，50-60Hz。
工作环境：室内（无潮湿，无阳光直射，无腐蚀性气体向左转|向右转
可储存十套常规程序数据和一套组合程序，工作方式有定时和计数两种。
组合程序最多可以由十套常规程序组成，可选择循环或不循环工作模式。
超温保护及报警功能。
超声波输出强度自动限定功能。
定时方式工作时间定时：0～99小时59分59秒。
计数方式超声工作次数：0～9999次。
超声时间范围：0～99小时59分59秒。
间隙时间范围：0～99小时59分59秒。间隙时间=0为超声连续工作。
温度控制精度：±1℃。
工作电压：VAC100-240，50-60Hz。
工作环境：室内（无潮湿，无阳光直射，无腐蚀性气体向左转|向右转
如何选购超声波细胞破碎仪|上海嘉鹏科技有限公司123
感性的asd23562021-07-22
在电脑上如何登陆INS方法
第一步：登陆脸谱vpn加速器官网
第二步：登陆客户端
第三步：连接所需要的线路
第四步：点击启动连接即可
第五步：可以在百度查询IP是否改变
账号lpvpn 密码lpvpn
第一步：登陆脸谱vpn加速器官网
第二步：登陆客户端
第三步：连接所需要的线路
第四步：点击启动连接即可
第五步：可以在百度查询IP是否改变
账号lpvpn 密码lpvpn
超声波细胞破碎仪对身体有害吗？ 泰速尔123
sen49532021-07-26
对孕妇多少有点影响，男人没有关系的
超声破碎仪使用说明123
音色tz42021-08-02
黑的调电流的，红的测试，蓝的是按下复位按钮，蓝色旁边绿色那个凸出来是动作指示，蓝色的上面十字长的指向H是手动复位，把它按下去往左旋到指向A是自动复位。
关于细菌超声破碎的一些经验总结123
陡变吧GEK2021-07-23
一是看破碎后菌液的浑浊程度，较清澈的是破碎比较完全的
二是镜检染色观察计数，破碎完全的基本看不到完整的菌体，都是细胞碎片
三是测菌液的电导率，根据电导率大小来判断。
二是镜检染色观察计数，破碎完全的基本看不到完整的菌体，都是细胞碎片
三是测菌液的电导率，根据电导率大小来判断。
一种用于dna和rna提取的细胞破碎仪的制作方法123
duzhengde2021-08-12
能用超声破碎仪提取总RNA和基因组DNA吗？
我问了两个实验员，一个说可以，一个说不行。
说不行的理由：1：会把RNA和DNA打断。2：超声破碎过程中会产热，会降解RNA。
请问有人用过破碎仪提取总RNA或基因组DNA吗？如果能，请问电压调到多少？还有哪些要求。
另外关于破碎仪消毒的问题，能用酒精棉球擦拭，再用无菌棉片檫干，再放入trizol中超声破碎吗？还有没有更好的方法？
谢谢！
我问了两个实验员，一个说可以，一个说不行。
说不行的理由：1：会把RNA和DNA打断。2：超声破碎过程中会产热，会降解RNA。
请问有人用过破碎仪提取总RNA或基因组DNA吗？如果能，请问电压调到多少？还有哪些要求。
另外关于破碎仪消毒的问题，能用酒精棉球擦拭，再用无菌棉片檫干，再放入trizol中超声破碎吗？还有没有更好的方法？
谢谢！
超声波细胞破碎仪使用注意事项:新芝生物123
牛牛n7Q2021-08-06
进行活细胞植发技术前5天，不要使用阿司匹林药物，进行活细胞植发技术前要对身体进行检查，保证身体健康，精神正常，而且手术者要正确看待手术效果。女性在进行活细胞注射前要避开月经期，而且在手术前要洗澡，保持身体的清洁。在进行植发前要保证手术部位吴局部感染病灶。
上海供应非接触式超声破碎仪丨非接触式全自动超声破碎仪价格 ...123
兔儿爷嗻nk32017-10-12
时间长了容易发热烧坏。
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