合同研究|技术与诀窍技术与诀窍JPT 开发了多种基于肽的独特专有技术和产品，例如临床肽和库、肽微阵列、肽 ELISA、蛋白肽和肽文库。这些技术能够有效地适应涉及药物发现、多肽结构的鉴定和优化、免疫疗法和疫苗开发以及生物医学研究不同领域的蛋白质组学的研究和开发项目。我们在生物信息学、化学信息学和药物化学方面的强大背景，以及我们坚实的轨道在管理研发合作方面的记录使 JPT 成为基于肽的药物发现、免疫疗法和疫苗开发以及分子诊断等领域的肽项目的知名合作伙伴。看看我们的专有技术：Biological Assays & ScreeningPeptide Microarray-based ProfilingPeptide ELISA-based验证质量基于光谱的发现化学药物化学多肽化学超高通量合成数据评估与统计生物信息学、化学信息学、分子建模合同研究|蛋白质组学|功能蛋白质组学功能蛋白质组学功能蛋白质组学旨在在蛋白质组范围内阐明蛋白质或蛋白质组和类别的生物学功能。这包括酶活性的表征以及蛋白质/蛋白质相互作用和翻译后修饰在蛋白质。结合我们的技术和专业知识，我们能够绘制由于细胞处理或环境条件变化而导致的信号通路变化。这可以通过大规模测量激酶、磷酸酶、蛋白酶或其他蛋白质修饰酶的酶活性或通过测量蛋白质的表观遗传修饰来完成。我们还开发了评估和呈现复杂数据集的创新方法，这至关重要用于实验结果的最佳利用。 1：改编自 Rauh 等人，Nature Comm (2013)合同研究|蛋白质组学ProteomicsJPT 为蛋白质组学界提供了大量的创新研究工具。除了使用高通量化学和分析方法来加速从生物样本中发现新的生物标志物外，JPT 还开发了一种新的基于质谱的肽标准技术，用于临床和靶向蛋白质组学中目标蛋白质的绝对定量。此外，JPT 在功能蛋白质组学领域拥有长达十年的提供服务和项目专业知识的记录，范围从阐明蛋白质-蛋白质相互作用和酶活性到表观遗传调控。基于质谱的蛋白质组学 功能蛋白质组学应用 应用目标发现和验证：我们评估您的目标蛋白在生物网络中的作用在一项实验中从患者血液样本中提取肿瘤相关抗原，并将数量与翻译后相关联al 修饰状态与临床数据临床监测：监测治疗干预的效果和疾病状态对于理解和描述应用药物对靶蛋白的影响至关重要；尤其是大量相关蛋白质的平行定量将促进个性化医学方法、患者分层和伴随诊断开发蛋白质组的蛋白质和蛋白质修饰状态的蛋白质组广泛定量酶活性或酶家族的蛋白质组广泛表征优点优点专有的高通量肽技术用于蛋白质的高度多重鉴定独特的方法用于蛋白质的绝对和多重量化翻译后修饰的覆盖范围快速访问整个人类蛋白质组中的数十万种肽参考文献参考文献Prosit：深度学习对肽串联质谱的蛋白质组范围预测Gessulat 等人，Nature Methods (2019)Dev基于靶向质谱法定量富含突触后密度 (PSD) 的蛋白质方法的进展 Wilson 等人，蛋白质组学 (2019)29 种健康人体组织的深层蛋白质组学和转录组丰度图谱Wang 等人，分子系统生物学 (2019) 采矿用于蛋白质瓜氨酸化的人体组织蛋白质组Lee 等人，分子与细胞蛋白质组学 (2018)ProteomeTools：使用合成肽通过 LC-MS/MS 对 21 种翻译后蛋白质修饰进行系统表征Zolg 等人，分子与细胞蛋白质组学 (2018) 组蛋白分析质谱法的修改 Völker-Albert 等人，蛋白质科学的当前方案 (2018)更多参考资料 Loading...

Histone Peptide H4 44:63 pS47

Product Code: SP-His_0253

C-terminally biotinylated synthetic Histone peptide carrying 1 modification (pS47) derived from human Histone H4 (Swiss-Prot Id(s): P62805). Amino acid sequence: Ac-KRI-pS-GLIYEETRGVLKVFLE-Lys(Biotinoyl)-NH2Amount: 1 vial containing 10nmol (appr. 35µg)Purity: >70% (HPLC-MS)Delivery format: Freeze-dried in amber type glass vialsApplication(s): Western Blot, Binding experiments, ELISA, Enzyme kinetics, Pull down assays, Substrate SpecifityIndication(s)/Topic(s): Cancer, Phosphatase,Kinase, Methyltransferase, Acetyltransferase, Histones, Phosphatase, Gene regulation & expression, Epigenetics, Cell signaling, Nucleosome, AgingDelivery time: 2-5 days

JPTs Histone Code Peptides As histones are a major focus of epigenetics, JPTs computational scientists compiled a unique library of synthetic posttranslationally modified and unmodified human histone peptides that is now available as individual biotinylated peptides. The peptide library consists of more than seven hundred 20mer peptides from 29 histone families (e.g. H2A[1,19,85...], H2B[1,22,42...], H3[1,23,43...], H4[1,14,44...]). Peptides carry PTMs at reported sites (e.g. acetylation, methylation, phosphorylation, citrullination) in various combinations representing natural variants. JPTs highly skilled and committed scientific staff ensures that the most appropriate methods and techniques are selected for every synthesis project. All of JPTs catalog peptides are provided with HPLC-MS analyses to confirm the identity and demonstrate the high quality of our peptides.

Benefits of JPTs Histone Code Peptides - Whole production performed in a regulated European environment according to DIN EN ISO 9001:2015 & GCLP standards; audits welcome! - For high-throughput screening the whole Histone Code Peptide library is also available displayed on peptide microarrays- Provision of freeze dried aliquots for enhanced stability - Proven track record for many applications

Histone Peptide H4 44:63 pS47

Selected References for Histone Peptide Tools \"Histone H2A and H4 N-Terminal Tails are Positioned by the MEP50 WD-Repeat Protein for Efficient Methylation by the PRMT5 Arginine Methyltransferase\"Burgos et al., J. Biol. Chem. (2015) - PMID: 25713080 \"H3K23me2 is a New Heterochromatic Mark in Caenorhabditis elegans\" Vandamme et al., Nucleic Acids Res. (2015) - PMID: 26476455\"Structure of the Arginine Methyltransferase PRMT5-MEP50 Reveals a Mechanism for Substrate Specificity\"Ho et al. PLOS ONE (2013) - PMID:23451136\"Use of High-Density Histone Peptide Arrays for Parsing the Specificity of a Histone-Modifying Enzyme Complex\"Shechter et al, Application Note (2013) (full text)\"Comprehensive Peptide Microarrays for Histone Research\"Schnatbaum et al, 32th EPS (2012) (full text)

Application Notes\"Use of High-Density Histone Peptide Arrays for Parsing the Specificity of a Histone-Modifying Enzyme Complex\"Shechter et al. Application Note (2013) (full text)\"Comprehensive Characterization of Antibodies Directed towards Epigenetic Histone-Modifications\" Masch et al. Application Note (2015) (full text)

Testimonial for Histone Code Peptide Microarray \"JPTs new extremely high-density histone peptide array is a dramatic leap forward for analysis of the histone code hypothesis. The complete sequence coverage across core histones and histone variants as well as the presence of common and rare histone post-translational modifications\"alone and in combination\" makes this an unparalleled tool for studying the \"writers\" and \"readers\" of the histone code. My laboratory has used these arrays to ask the sequence and PTM dependence of a histone writer enzyme and we have discovered an activity specifying code that will keep us busy for years to come.\"David Shechter, PhD, Department of Biochemistry, Albert Einstein College of Medicine, YU (NY, USA)

Histone Peptide H4 44:63 pS47

Technical Data:

Histone Family	Histone H4
Modification	Phosphorylation
Sequence Position	Core
Isoform	H4
Biotinylation	Biotinylated Peptide
Application	ELISA
Protein Name	Histones
Organism	Homo sapiens (Human)
Indication / Topic	AcetyltransferaseEpigeneticsKinase
Number of peptides	1

Further Information:

Amount	1 vial containing 10nmol (appr. 35µg)
Specifications	C-terminally biotinylated synthetic Histone peptide carrying 1 modification (pS47)
Documentation	Product Datasheet
Swiss-Prot ID	P62805
Sequence	Ac-KRI-pS-GLIYEETRGVLKVFLE-Lys(Biotinoyl)-NH2