Excellgen/Nuclear receptor coactivator 1, SRC1-RID Biotinylated / His Tag removed/RP-133/5,000 U
| Product Name | Nuclear receptor coactivator 1, SRC1-RID Biotinylated |
| Size | 5,000 U |
| Description | Steroid receptor coactivator 1 (SRC1) is a transcriptional coactivoator that mediates the activating functions of many of the nuclear hormone receptors. It is also known as NCoA1 and is a member of the SRC/p160 coactivator family (1). SRC1 has been shown to be over expressed in some cancers (2). SRC1 is a 160 kDa protein that contains several LXXLL motifs, which are involved in nuclear receptor interaction (3). The region 627-786 contains 3 LXXLL motifs that are involved in interaction with nuclear hormone receptors (4,5) and has been previously used in assays detecting ligand-dependent receptor-cofactor interactions (6). This protein does not contain His tag and will be useful for assays where a native untagged protein is desired. Lane 1 corresponds to 6His-tagged and biotinylated SRC-RID; Lane 2 corresponds to biotinylated SRC-RID without 6His tag. |
| Applications | protein-protein interaction assays, particularly suitable for high throughput screening (HTS) assays. |
| Source | E. coli |
| Fusion Tag | None |
| Purification Method | FPLC |
| Unit Definition (Activity) | 1 unit equals to 1 nanogram of purified protein. 100 units are sufficient for a protein-protein interaction assay. |
| Purity | ~ 95% as determined by SDS-PAGE |
| Gene ID | 8648 |
| Accession # | NM_003743 |
| Gene Name | NCOA1 |
| Gene Synonyms | SRC1; KAT13A; RIP160; F-SRC-1; bHLHe42; bHLHe74 |
| MW | 19.8 kDa |
| Protein Sequence | SQTSHKLVQL LTTTAEQQLR HADIDTSCKD VLSCTGTSNS ASANSSGGSC PSSHSSLTERHKILHRLLQE GSPSDITTLS VEPDKKDSAS TSVSVTGQVQ GNSSIKLELD ASKKKESKDHQLLRYLLDKD EKDLRSTPNL SLDDVKVKVE KKEQMDPCNT |
| Storage Buffer | 20 mM Tris-HCl, pH 8.0, 250 mM NaCl, 50% Glycerol |
| Storage | -20 to -80 °C. |
| Shipping | 4°C or dry ice |
| References | 1. Onate, S. et al., (1995) Science 270, 1354-13572. Yanase, T., et al., (2004) Intern. Med. 43, 368-3733. Heery, D. et al., (1997) Nature 387, 733-7364. Xu, J. and Li, Q., (2003) Mol. Endocrinol. 17, 1681-16925. Bai, Y. and Giguere, V., (2003) Mol. Endocrinol. 17, 589-5996. Cho, M. et al., (2003) J. Biochem. Mol. Biol. 36, 207-213 |
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