Description

The ImmunoStar NK1R antiserum was quality control tested using standard immunohistochemical methods in rat brain using biotin/avidin-HRP techniques. Specificity of the antiserum was demonstrated by soluble pre-adsorption and Western blot. Tissue staining is completely eliminated by pretreatment of the diluted antibody with 10 mg of rat NK1R peptide residues 393-407.

Photo Description: IHC image of neurons staining for the neurokinin 1 receptor in the rat striatum. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

Host: Rabbit

Quantity / Volume: 100 µL

State: Lyophilized Whole Serum

Reacts With: Rat

Availability: In Stock

Alternate Names: Tachykinin receptor 1; NK-1 receptor; SPR, Substance-P receptor; tachykinin peptide hormone; Tacr1r, anti-NK-1

Gene Symbol / ID, Accession #: Tacr1, 24807RRID: AB_10013880