I.U.B.: 3.4.22.2

Papain is a sulfhydryl protease from Carica papaya latex. It has a molecular weight of 23 kDa and an optimum pH range of 6.0-7.0. The action of papain on leucine methyl ester produces an insoluble polyleucine peptide. Papain breaks down the intercellular matrix of cartilage. Papain is activated by cysteine, sulfide, and sulfite. Stabilizing agents are EDTA, cysteine and dimercaptoethanol.

Stability/Storage: Stable for 6-12 months at 2-8°C. Do not freeze aqueous suspensions.

Technical Notes: Papain preparations should be incubated in the activation solution before use to ensure full activity. Applications include antibody fragmentation and primary/neural cell isolation.

Unit Definition: One Unit hydrolyzes one micromole of benzoyl-L-arginine ethyl ester per minute at 25°C, pH 6.2, after activation in a solution containing 1.1 mM EDTA, 0.067 mM mercaptoethanol and 5.5 mM cysteine-HCl for 30 minutes.

Related Products:

Cell Isolation Optimizimg System (CIT)Hepatocyte Isolation System (HIS)Neonatal Cardiomyocyte Isolation System (NCIS)Papain Dissociiation System (PDS/PDS2)