您知悉并同意，丁香通平台展示产品并非为平台自身产品，您发起询价或试用申请后，平台将会把您已提供或按要求提供的相关信息同步提供至所咨询的具体产品商家，由商家为您提供具体产品的价格咨询或产品试用注意事项及相关试用产品或完成相应购买，因此您知悉并授权平台将您的信息进行同步共享。您有权拒绝，但您知悉将无法参加询价或试用活动。         舜冉(上海)生物SERANA胎牛血清中国区总代理.           Gibco胎牛血清、Hyclone血清、BI胎牛血清、SIGMA血清、PAN胎牛血清、BOV胎牛血清、Corning胎牛血清、PAA胎牛血清、NTC胎牛血清、HAKATA胎牛血清等常年备货，现货充足，欢迎各位老师选购！        舜冉所出售的血清，均为原装进口，一个月内不满意，包退换！  舜冉(上海)生物所销售的 Gibco胎牛血清，全部都为进口原装，提供产品COA质检报告及批次报告。舜冉（上海）生物血清库主营品牌：gibco品牌，Bovogen品牌，hyclone品牌，ATCC品牌，Sigma品牌，abcam品牌，CST品牌，SBI品牌，BI品牌，SERANA品牌，PAA品牌，PAN品牌。主营：进口胎牛血清，gibco10099-141胎牛血清，gibco10091-148胎牛血清，小牛血清，胰酶，双抗，细胞系，代购ATCC原代细胞，gibco羊水培养基（货号：11269-016），sigma人AB血清。（需要提供海关报关单等相关事宜，请咨询本公司）舜冉（上海）生物科技有限公司 Gibco fetal bovine serum (FBS): frequently asked questions  What is the difference between fetal bovine serum and fetal calf serum?There is no difference. Both terms refer to serum from an unborn cow.How is dialyzed FBS processed?It is dialyzed by tangential flow filtration against 0.15 M NaCl using a 10,000 molecular weight cutoff membrane until the glucose level is less than 5 mg/dL. Because this is not exhaustive dialysis, low molecular weight dialyzable components such as amino acids may not becompletely removed. Exhaustive dialysis is not performed because it can result in precipitation and inactivation of serum peptides. What is the difference between Certified and Qualified FBS?The serum is from the same source, collected and processed in the same way. The differences are in the testing performed on the serum and the specifications for release. Certified serum undergoes additional biochemical and hormonal testing. Certified FBS has a lower concentration of endotoxin (5 EU/mL or less) and hemoglobin (15 mg/dL or less). What does International Serum Industry Association (ISIA) traceability certification mean?It means the company maintains all auditable history of quality and quantity of materials from the point of collection (including specific animal numbers for donor products), and documentation supporting all stages of processing and transportation or commercial transactions. How do I heat-inactivate serum?Heat it at 56°C in a water bath for 30 minutes and swirl the bottle every 10 minutes or so. For accuracy, use a second bottle of similar size as a control and add an equivalent volume of water to the control bottle. Place a thermometer in the control bottle to see when 56°C is reached. Set your timer for 30 minutes at this point.Why would I heat-inactivate serum?Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.What is the importance of gamma-irradiated sera? Gamma irradiation is recognized as an effective method for inactivating viruses in animal-origin material. Based onUSDA regulations for the general requirements for antibody products (9CFR, Section 113.450), the minimum dosage for treating blood derivatives of animal origin is 25 kGy (kilogray). Certain European countries require productsto be treated prior to importation with a minimum dose of 25 kGy.We will gamma-irradiate serum on request. We have validated a process for using gamma irradiation in the range of 30–45 kGy to inactivate the most common bovine viruses and mycoplasmas that may be present in FBS. The level of inactivation is 6–8 logs for viruses and 6–7 logsfor mycoplasmas. We have also demonstrated that physiochemical properties and cell culture performance of serum are not altered by gamma irradiation atlevels of 30–40 kGy.  Can I get custom serum products for my specific research requirements?Yes, we offer a wide range of custom options. Please contact our serum specialists to discuss your application and research needs. We will work with you to supply sera that is specially processed and tested to meet your individual requirements. Some examples of our custom capabilities are heat inactivation, gamma irradiation, and testing for tetracycline residues. What is the shelf life of FBS?Most FBS products that are unopened and stored in a freezer have a 5-year shelf life. Also, you can refer to the expiration date on the bottle and the Certificate of Analysis for this information. Why does material precipitate in FBS upon storage in the refrigerator?Gibco™ FBS is not pre-aged. When stored at 2–8°C, various proteins and lipoproteins in the serum (e.g., cold agglutinins, fibrinogen, vitronectin) may aggregate and form visible material or turbidity; however, this should not affect serum performance. We recommend that you store FBS at–20°C and avoid repeated freeze-thaw cycles.My FBS contains flocculence. What could it be?Flocculence may appear in FBS for a variety of reasons. The most common reason is the denaturation of serum lipoproteins. You may observe fibrin, a clot-forming protein, after the serum has been thawed. This should not affect serum performance.To remove flocculence, transfer the serum to sterile tubes and centrifuge the material briefly at 400 x g, then filter the resulting supernatant along with your media. Do not attempt to filter serum containing flocculence, as it may clog filters. Is serum sterile?Fetal bovine serum is triple-filtered through 0.1 µm filters. Bovine serum is filtered through 0.2 µm filters.Is serum tetracycline-free?Our limit of detection for tetracycline is 19.7 ng/mL, and tetracycline must be \"undetectable” to pass inspection, but we cannot say that it is tetracycline-free. How should serum be thawed?Serum should be thawed overnight at 2–8°C.If my FBS arrives partially thawed, can I still use it? All Gibco FBS is shipped frozen and packaged in dry ice, so it should arrive frozen. You can still confidently use FBS that is partially thawed, if at least two-thirds of it is frozen. Can I aliquot and refreeze serum?Yes, after the overnight thaw, you can aliquot the serum into smaller, single-use aliquots and refreeze it.How long can I store thawed serum at 2–8°C? Serum should be used within 2–4 weeks when stored at 2–8°C. How can I minimize lot-to-lot variability?You can test each new lot to be sure that it works for your specific applications. Alternatively, our serum sales team can determine the most consistent, highest-performing serum lot available using the Gibco™ iMatch™ Sera Lot Matching Tool. What sizes of packaging are available?Gibco FBS is available in 50 mL, 100 mL, 500 mL, and 1,000 mL bottles.How can I sample FBS or set up a reserve?Your serum sales representative will be happy to assist you in getting a reserve set up for a specific lot or testing to see what lot may work best for you.Alternatively, you can request a FBS sample yourself atthermofisher.com/fbssamplerequest.1、产品验收：（1） 血清内外包装均应完好，无破损、裂缝、溢渗等现象；（2）血清到货时应为冷冻或冰水混合状态，不应完全融化；（3）若出现上述现象，请拍照取证后及时与我们联系，以便进行更换。2、血清的解冻：（1）请在2℃~8℃环境中解冻，不宜在较高温度下进行解冻，解冻温度较高会导致血清浑浊，沉淀增加，品质下降；（2） 血清解冻的过程中请不时摇匀（小心勿造成气泡），使血清成分和温度均匀，从而减少沉淀的产生；（3） 血清解冻后应尽快用完，尽量避免反复冻融；（4）血清不宜在室温中长时间放置，使用后尽快放回2℃～8℃中，2℃～8℃存放时，请勿超过一个月。（5） 高温解冻、剧烈摇晃、反复冻融、放置时间过长等，均会导致血清品质的降低。3、本产品仅供研究或进一步生产使用，不用于诊断或治疗；舜冉（上海）生物，折扣多多，有买就有送!!优势出售原装进口血清：GIBCO 10099-141、GIBCO 16000-044、GIBCO 10270-106（南美胎牛血清）、GIBCO 10091-148、GIBCO 16050-122（马血清）；BOVOGEN胎牛血清SFBS、新生牛血清SNCS；PAN 胎牛血清ST30-3302、P30-2602（ES级）；Hyclone 南美血清Sv30087.02、澳洲源SH30084.03、乌拉圭血清SV30087.03；NTC胎牛血清；BI胎牛血清南美血源04-001-1ACS；SIGMA人血清F4522、SIGMA胎牛血清F2442；GEMINI胎牛血清900-108、100-500、科研用人AB血清100-512；康宁特级胎牛血清35-076-CV；SBI 无外泌体血清EXO-FBS-50A-1；CLARK胎牛血清FB15015、FB25015、FB35015（透析型）、NBC15015（新生牛血清）等优质血清，欢迎来电详询。★CQ★舜冉（上海）生物优势批发：GIBCO 培养基、胰酶（100ml和500ml）、GIBCO胶原酶（I型、II型、IV型）、GIBCO 双抗15140-122；hyclone培养基、胰酶和双抗；ELISA试剂盒；GIBCO 1640培养基；GIBCO DMEM/F12；GIBCODMEM；PBS缓冲液SH30256.01和C10010500BT；BD Matrigel基质胶；完全培养基500ml；西班牙原装琼脂糖 Agarose；LIPO2000（1.5ml 0.75ml）；LIPO3000（0.75ml 1.5ml）；GIBCO B27；trizol试剂等，价格美丽，质量第一，批量走货，价格更优，超高性价比产品，欢迎咨询。活动进行中，培养基优惠促销，胰酶双抗买十送一活动，胎牛血清折扣多多，有买就有送，欢迎来电详询，更多人AB血清、胎牛血清、豚鼠血清、兔血清、绵羊血清、山羊血清、封闭驴血清、大鼠血清、小鼠血清。代购ATCC原代细胞，SBI试剂盒，等试剂，请来电咨询Gibco澳洲胎牛血清  舜冉（上海）生物科技有限公司 风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。