Description
DMEM/F-12 (Dulbeccos Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool. This DMEM/F-12 is modified as follows:
| With | Without |
| • L-glutamine | • HEPES |
The complete formulation is available.Using DMEM/F-12DMEM/F-12 is a 1:1 mixture of DMEM and Hams F-12. This formulation combines DMEMs high concentrations of glucose, amino acids, and vitamins with F-12s wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO
2 environment to maintain physiological pH.cGMP manufacturing and quality systemDMEM/F-12 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable DMEM/F-12 product made in our Scotland facility (11320-074). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Specifications
Glutamine:| L-Glutamine |
Phenol Red Indicator:| Phenol Red |
Form:| Liquid |
HEPES Buffer:| No HEPES |
Sodium Pyruvate Additive:| Sodium Pyruvate |
Serum Supplementation:| Standard Serum Supplementation |
Glucose:| High Glucose |
Product Line:| Gibco® |
Product Size:| 500 mL |
Volume (Metric):| 500 ml |
Green Features:| Sustainable packaging |
Shipping Condition:| Room Temperature |
Contents & storage
Storage conditions: 2°C to 8°C (protect from light)Shipping conditions: AmbientShelf life: 12 months from date of manufacture
Media formulations
11320 - DMEM/F-12
