Description: TNF-alpha, Human, mAb 52B83, FITC

The monoclonal antibody 52B83 reacts with tumor necrosis factor alpha (TNF-alpha). TNF-alpha is a homotrimeric 17 kDa protein, that interacts with either one of the two types of TNF-receptors, termed I and II, leading to receptor cross-linking and signal transduction. The receptors differ strongly in their intra-cellular signaling pathways.TNF-alpha was originally described as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Furthermore,- TNF-alpha is found to be a central mediator in many inflammatory and immunological processes. It can be induced by various products of micro-organisms and by various cytokines leading to expression of a wide variety of- cytokines. The pro-inflammatory properties of- TNF-alpha play a central role in several auto-immune diseases such as rheumatoid arthritis and inhibition by neutralizing molecules have been shown to be beneficial in patients.

Specifications:

Catalog number Product type Quantity Formulation Conjugate Application Application Notes Use Isotype Species Cross reactivity Alias References Storage and stability Precautions Disease

HM2010F

Monoclonal antibodies

100 µg

1 ml (100 μg/ml) 0.2 μm filtered protein G purified FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide.

FITC

Flow cytometry, Frozen sections, Immuno assays, Western blot

FC: Antibody 52B83 was used to stain soluble TNF bound to TNF receptors.- Cells were fixed in PBA containing 0.2% formaldehyde (Ref. 3)W: A reduced sample treatment and 15% SDS-Page was used. The band size observed is 17 kDa (Ref.4).IHC-F:- 6 µm tissue sections were fixed in acetone for 10 minutes (Ref. 4).

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Mouse IgG1

Human

Guinea pig - Yes, Mouse - Yes, Rhesus monkey - Yes

Tumor Necrosis Factor-alpha, TNF, TNF-SF2, cachectin

1. Bradding, P et al; Interleukin-4, -5, and -6 and Tumor Necrosis Factor-alpha in normal and asthmatic airways: Evidence for the human mast cell as a source of these cytokines. Am J Respir Cell Mol Biol 1994, 10: 4712. Bradding, P et al: TNFalpha is localized to nasal mucosal mast cells and is released in acute allergic rhinitis. Clin Exp Allergy 1995, 25: 4063. Gerspach, J et al; Detection of membrane-bound Tumor Necrosis Factor (TNF): an analysis of TNF-specific reagents. Microsc Res Tech 2000, 50: 2434. Laan van der, N et al; Tumor necrosis factor alpha (TNFalpha) in human skin: a comparison of different antibodies for immunohistochemistry. Arch Dermatol Res 2001, 293: 2265. Lee, E et al; Clinical and immunohistochemical characteristics of mucoceles. Ann Dermatol 2009, 21:3456. Brunner, P et al; Infliximab induces downregulation of the IL-12/IL-23 axis in 6-sulfo-LacNac (slan)1 dendritic cells and macrophages. J Allergy Clin Immunol 2013, 132:1184

Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.

Autoimmunity, Gastroenterology