Description:

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Rat IL-1alpha ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat IL-1alpha proteins within the range of 16-1000 pg/ml. The Rat IL-1alpha ELISA is capable of recognizing both recombinant and naturally produced Rat IL-1alpha proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 5% cross reactivity.• Murine IL-1alphaThe antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. • Human IL-1alpha, IL-1beta• Murine IL-1beta• Rat GM-CSF, GRObeta, GRO/KC, IFN-gamma, IL-1beta, IL-2, IL-3beta, IL-4, IL-6, IL-7, IL-10, IL-13, RANTES, SCF, TNF-alpha

Additional Information:

Name Related Product Names Gene Symbol Gene Family Gene Fullname Gene id Alias Symbols Protein Name Description of Target Swissprot ID Protein Accession Num Nucleotide Accession Num Species Reactivity Assay Info Sensitivity Kit Range Standard Range Sample Type Product Format Specimen/Volumes Swiss Prot Number Specificity Applications GI Number Format Stability Reconstitution and Storage Reactivity Kit Size Kit Principle Kit Component Storage Lead Time Intended Use

Rat IL-1alpha ELISA Kit

Interleukin-1 alpha, IL-1 alpha

IL1A

ENDOLIG

interleukin 1 alpha

24493

IL-1 alpha, Interleukin-1 alpha

Interleukin-1 alpha

proinflammatory cytokine [RGD, Feb 2006]

P16598

NP_058715.1

NM_017019.1

Rat

Quantitative Colorimentric Sandwich ELISA

16 pg/mL

16-1,000 pg/mL

16-1000 pg/ml

Cell lysates, sera and plasma

1 x 96-Well Plate or 12 x 8-Well Strips

Refer to Manual

P16598

The Rat IL-1α ELISA is capable of recognizing both recombinant and naturally produced Rat IL-1α proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 5% cross reactivity.• Murine: IL-1αThe antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.• Human: IL-1α, IL-1β• Murine: IL-1β• Rat: GM-CSF, GROβ, GRO/KC, IFN-γ, IL-1β, IL-2, IL-3β, IL-4, IL-6, IL-7, IL-10, IL-13, RANTES, SCF, TNF-α

ELISA

24493

ELISA Kit. Refer to manual for specific component formats

6 months

4C/6 Months

Rat

12 x 8-Well Microstrips

The Aviva Rat IL-1alpha ELISA Kit contains the components necessary for quantitative determination of natural or recombinant rIL-1alphaconcentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a \"Sandwich\" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a \"sandwich\" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator.The capture antibodies coated to the bottom of each well are specific for a particular epitope on theRatIL-1alpha cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and \"sandwiching\" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetricreaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.

ComponentsAmount Capture Antibody Coated Microplate 96 Wells (12 x 8) Ready-to-Use Streptavidin-HRP 12 mL Ready-to-Use Substrate 12 mL Stop Solution 12 mL Wash Buffer (15x) 50 mL Protein Standard Diluent 12 mL Sample Diluent 12 mL Detection Antibody Diluent 12 mL Biotin-Labeled Detection Antibody Lyophilized Protein Standard Lyophilized (100 ng)

4°C/6 Months

Domestic: within 1 week deliveryInternational: 1 week

Research Use Only