Zyagen/NP-1 Recombinant Protein/8058/1 Ea-蚂蚁淘生物

Background

Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The ?-defensins which include NP-1, NP-2 and NP-3, are distinguished from the ?-defensins by the pairing of their three disulfide bonds. In addition to antimicrobial activity, NP-1 exhibits chemotactic activity on dendritic cells. NP-1 is expressed as the C-terminal portion of an inactive precursor protein, which also contains a 19 amino acid N-terminal signal sequence and a 45 amino acid polypeptide. NP-1 contains a six-cysteine motif that forms three intra-molecular disulfide bonds. Recombinant human NP-1 is a 3.4 kDa protein containing 30 amino acid residues.

Specifications

Additional Names

Neutrophil Peptide-1, alpha Defensin-1, HNP-1, Cryptdin

Preparation

Determined by its ability to chemoattract immature dendritic cells using a concentration of 1.0 - 10.0 ng/ml.

Location

ACYCRIPACI AGERRYGYCI YQGRLWAFCC

Source

E. coli

Reactivity

n/a

Tested Application

n/a

Storage

The lyophilized NP-1 recombinant protein is stable for at least 2 years from date of receipt at -20ËšC. Reconstituted NP-1 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20ËšC. As with any protein, exposing NP-1 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Species Reactivity

n/a

Purity

Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).