Agrisera/Rhodanese/cell cycle control phosphatase superfamily protein/AS15 2900/-蚂蚁淘生物


product information
Background		Rhodanese/cell cycle control phosphatase superfamily protein is localized in chloroplasts.
Immunogen		KLH-conjugated synthetic peptide derived from Arabidopsis thaliana ATCaS sequence UniProt: F4J9G2, TAIR:AT3G59780
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Lyophilized
Quantity		50 µl
Reconstitution		For reconstitution add 50 µl of sterile water.
Storage		Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		Western blot (WB)
Related products		Antibodies to proteins involved in photosynthesisPlant protein extraction buffer Secondary antibodies
Additional information

application information
Recommended dilution		1:1000 (WB)
Expected \| apparent MW		73.9 \| 69 kDa
Confirmed reactivity		Arabidopsis thaliana
Predicted reactivity		Brassica napus, Gossypium arboreum
Not reactive in		no confirmed exceptions from predicted reactivity known in the moment
Additional information
Selected references		To be added when available, antibody released in October 2016.

Application example

$\"western$

10, 15, 20 µg of total protein from Arabidopsis thaliana chloroplast and thylakoids were separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose. Blots were blocked with 10% Non fat dairy milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was washed twice, then washed three times for 15 minutes in TBS-T at RT weith agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 in TBST for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 1 minute.

Courtesy of Dr. Rikard Fristedt VU University Amsterdam, The Netherlands

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