Antibodies-Online/beta-2 Microglobulin ELISA Kit (beta-2-Microglobulin)/ABIN2641816/5 x 96 tests
Antigen - B2M
- b2m
- B2MG
- Ly-m11
- beta2-m
- beta2m
- b2m-W01
- b2m-W03
- b2m-Z01
- b2m-Z02
- b2m-Z03
- bwm
- wu:fa94c05
- wu:fb10a09
- beta-2-microglobulin
- beta-2 microglobulin
- Beta-2-microglobulin-like
- B2M
- b2m
- B2m
- LOC100350679
Alternatives Human beta-2 Microglobulin ELISA Kit Method Type Competition ELISA Detection Range 0.025-10 μg/mL Minimum Detection Limit 0.025 μg/mL Application ELISA Options Supplier Supplier Product No. ![\"Independent](\"https://www.ebiomall.com/images/antibodies_online/201710/029855_validation_logo_scale_105x150_029855_validation_logo.png\" "\\"Successfully") | - Successfully validated (ELISA)
- by Affina Biotechnologies, Inc
- No. #029855
- Date 04/02/2016
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| ![\"beta-2-Microglobulin](\"https://www.ebiomall.com/images/antibodies_online/201710/beta-2-Microglobulin+B2M+ELISA+Kit_aspect_300x200_abimg789651.png\") ![\"beta-2-Microglobulin](\"https://www.ebiomall.com/images/antibodies_online/201710/beta-2-Microglobulin+B2M+ELISA+Kit_aspect_300x200_abimg789652.png\") |
| Antigen | Human β2-microglobulin (B2M) |
| Lot Number | C738151804 |
| Method validated | ELISA |
| Positive Control | Human pooled serum (Biochemed, Lot#BC033016HSPMG) |
| Negative Control | Chicken serum (Biochemed, Lot#BC03316CSPMG) |
| Notes | β2-microglobulin was easily detected in diluted and undiluted positive samples (up to 8-fold dilutions). Very low signal was seen in the negative control. Spike showed very good recovery (~82%). |
| Controls | - Positive control: Human pooled serum (Biochemed, Lot#BC033016HSPMG)
- Negative control: Chicken serum (Biochemed, Lot#BC03316CSPMG)
- Standard curve: 0, 0.025, 0.1, 0.5, 2.5, 10 μg/mL β2-microglobulin provided in the ELISA kit
- Spike control: 2.5 μg/mL standard premixed with chicken serum in a 1:1 ratio
|
| Protocol | - 1. 50 μL of standard and samples were added to 96-well strip plates provided in the kit with 50 μL of HRP conjugate. All samples and standards were assayed in duplicate.
- 2. The microplate was covered and incubated at 37°C for 1 hr.
- 3. Content of the wells was discarded and wells were washed 3 times with 200 μl of washing solution.
- 4. 50 μl of Substrate A and Substrate B each was added to each well. The plate was covered and incubated at 37°C for 15 min.
- 5. 50 μl of the Stop Solution was added per well.
- 6. The optical density (OD value) of each well was read immediately using a microplate reader set to 450 nm.
- 7. The duplicate readings for each sample were averaged and the average zero standard optical density subtracted. The corrected average-value was tabulated as Average Absorbance. A standard curve was generated by plotting the mean OD value for each standard on the x-axis against the concentration on the Y-axis using CurveExpert 1.4 (CUSABIO). An exponential equation was used for the best fit through the points on the graph.
- 8. The CurveExpert Analyze feature was used to calculate human β2-microglobulin concentrations of the samples based on their Average Absorbance values.
|
| Experimental Notes | - The concentration of human β2-microglobulin in human and chicken sera was measured according to the manufacturer’s directions. |
