This antibody detects Drebrin A and E in samples from human, mouse, rat, guinea pig, and cat origins and Drebrin E only in chicken samples. Mouse, Rat, Human预测可用于: Chicken, Guinea pig, Cat, Monkey, Quail 常规说明

Abcam is committed to meeting high quality standards of ethical manufacturing and has decided to discontinue this product by June 2020 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We suggest ab60933 or ab178408 or ab176318 as possible replacements.

For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Drebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites, respectively. 组织特异性 Brain neurons. Also found in the heart, placenta, skeletal muscle, kidney and pancreas. Developmentally regulated brain protein antibody Developmentally-regulated brain protein antibody DKFZp434D064 antibody DREB_HUMAN antibody Drebrin 1 antibody Drebrin antibody Drebrin E antibody Drebrin E2 antibody Drebrin1 antibody DrebrinE antibody see all Western blot - Anti-Drebrin antibody [M2F6] (ab12350)This image is courtesy of an Abreview submitted by Dr Martin Broadstock All lanes : Anti-Drebrin antibody [M2F6] (ab12350) at 1/5000 dilutionLanes 1-4 : Trangenic (APP/PS1) male mouse cortical lysateLanes 5-8 : Wild-type male mouse cortical lysateLane 9 : Rat cortical lysateSecondaryAll lanes : Alexa-Fluor 680 conjugated goat anti-mouse IgGPredicted band size: 65 kDaObserved band size: 120,42,95 kDa why is the actual band size different from the predicted?Western blot showing detection of drebrin in mouse and rat cortical lysates by ab12350.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Drebrin antibody [M2F6] (ab12350)This image is courtesy of Carl Hobbs, King\'s College London, United Kingdom

IHC-P image of Drebrin staining on Human ovarian carcinoma sections using ab12350 (1:100). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21 C. ab12350 was diluted 1:100 in TBS buffer (containing BSA and Azide) and sections were then incubated with ab12350 for 16 hours at 21 C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Drebrin antibody [M2F6] (ab12350)This image is courtesy of Carl Hobbs, King\'s College London, United Kingdom ab12350 at 1/50 staining mouse brain tissue sections by IHC-P. The tissue fixed in 4% Formalin, diluted in Dulbecco A PBS with standard pwax processing. Citric acid 0.01M pH6 was used as antigen retrieval solution. The tissue sections were incubated with the antibody for 2 hours. A biotinylated goat polyclonal antibody was used as the secondary. The image shows the lateral ventricle showing high cytoplasmic positivity in migrating progenitor neurones in the subventricular space.There is a low level of positivity in the dendritic field of the striatum; this appears to be specific.An identical pattern of staining is seen in same area of rat brain. All non-migratory cells in the same field are negative. The Corpus Callosum is negative.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Drebrin antibody [M2F6] (ab12350)This image is courtesy of Carl Hobbs, King\'s College London, United Kingdom Immunohistochemistical detection (on formaldehyde/PFA-fixed paraffin-embedded sections) of Drebrin antibody [M2F6] (ab12350) on Quail Tissue sections (embryo d5/6 brain). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody: ab12350 incubated at 1/200 for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti mouse Igs (1/200). Immunohistochemistry - Free Floating - Anti-Drebrin antibody [M2F6] (ab12350)This image is courtesy of Sophie Pezet, Univ London Kings Coll, United Kingdom Immunofluorescent staining in the rat brain cerebellum with ab12350 mouse monoclonal Drebrin antibody. Drebrin-positive neurons and fibers are observed. Picture taken with X20 objective. Protocol: IHC free-floating protocol using 4% PFA fixed brain tissue. Rats were intracardially perfused with 4% PFA. Tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. Primary antibody ab12350 was used at 1/1000 incubated overnight at room temperature. Secondary antibody was Alexa Fluor 488 used at 1/1000, 2h incubation at room temperature. Image recoloured in Adobe photoshop. Overlay histogram showing SH-SY5Y cells stained with ab12350 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12350, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions. Western blot analysis of Drebrin expression in mouse brain (1) NIH/3T3 (2), Jurkat (3), HeLa (4), and PC12 (5) using Drebrin mAb (ab12350).

发表研究结果有使用 ab12350？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab12350 被引用在 27 文献中.

Zhao B et al. Microtubules Modulate F-actin Dynamics during Neuronal Polarization. Sci Rep 7:9583 (2017).PubMed: 28851982 Woo JA et al. Loss of function CHCHD10 mutations in cytoplasmic TDP-43 accumulation and synaptic integrity. Nat Commun 8:15558 (2017). MousePubMed: 28585542 Cho C et al. Early growth response-1-mediated down-regulation of drebrin correlates with loss of dendritic spines. J Neurochem 142:56-73 (2017).PubMed: 28369888 Tello-Lafoz M et al. Sorting nexin 27 interactome in T-lymphocytes identifies zona occludens-2 dynamic redistribution at the immune synapse. Traffic 18:491-504 (2017).PubMed: 28477369 Ambrosi C et al. Connexin43 Forms Supramolecular Complexes through Non-Overlapping Binding Sites for Drebrin, Tubulin, and ZO-1. PLoS One 11:e0157073 (2016).PubMed: 27280719 View all Publications for this product Blocking step BSA as blocking agent for 10 minute(s) Concentration: 1% Temperature: 21 C Blocking step BSA as blocking agent for 10 minute(s) Concentration: 1% Temperature: 21 C

Thank you for your reply and for providing that information. I have organized for a replacement vial of ab12350 to be send to you. As you purchased the antibody from Cedarlane, we need to send to them first and they will then ship it out to you. Hopefully you should have it by the end of the week. I do appreciate your patience in this matter and if there is anything else I can help you with, please let me know.

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I am sorry about all the emails, however I do need to make sure that I am sending a replacement to the correct place and since you ordered through Cedarlane, I cannot just look at the shipping address. The PO and order number you provided did not match anything in our system (I suspect these are Cedarlanes order information not Abcams), but I tracked what I believe to be your order through the lot number.However before send a replacement antibody, I do need you to confirm either the order date, maybe call Celarlane again and ask them to provide the PO# they used with Abcam. As soon as I can verify that I have the correct order, I will send the replacement.Once again, I do apologize for the delay in you receiving the replacement, but I do need to make sure that I have your order and not someone else s.I look forward to your reply.

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Thank you for your reply.I think I have found your original order and so will be able to get you the replacement next week. Before going further, could you confirm the date that the original order was placed and also confirm that you are ok with receiving a new vial of the same lot. As I mentioned in my previous email, we do not have a different lot that I can send.If you would prefer a different antibody, that would also be possible.I look forward to your reply and helping to resolve this situation.

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Thank you for your reply.I would be happy to send you a different lot of the antibody. Before I can do that though, I do need to have the original order information. Since you ordered via Cedarlane, would you please contact them and ask them to provide you with the order information they sent to Abcam. Once I have that information, I can go ahead and send the replacement.I look forward to your reply and resolving this situation.

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Thank you for your reply and for providing that information.Based on the information that you have provided, I believe the best way forward is to either replace or refund the antibody for you. Unfortunately we do not have another lot of ab12350 that I can send.Please let me know how you would you like to continue and if you could also provide either the Abcam order number or the PO# used to purchase the antibody, that would be very helpful.I look forward to your reply.

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Thank you for contacting Abcam.I am sorry about the issues that you are having with the current lot of ab12350 in IHC-P. The antibody is covered under our Abpromise for six months and is guaranteed to work in IHC-P on human samples.For our records would you able to let us know all the different conditions (primary antibody concentration, blocking, incubation times etc) that you have tried using the current lot of the antibody? I know you successfully used previous lots before, but there may be some protocol advice I could give.If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody. To replace the antibody could also provide either the Abcam order number or the PO# used to purchase the antibody?I look forward to your reply and helping you to resolve this issue.

Read More Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) Concentration: 5% Temperature: 23 C Blocking step BSA as blocking agent for 15 minute(s) Concentration: 2% Temperature: 25 C

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