MW: 198.2 Da CAS Registry #: 5144-89-8 Formula: C12H8N2 · H2O Description:1,10-phenanthroline (o-phenanthroline) is a chelator with high affinity for divalent metal ions. When complexed with copper, it possesses nuclease activity that has been used to study DNA-protein interactions. While not specific for DUBs, it has been shown to be a potent inhibitor of JAMM-type isopeptidases through chelation of the active site Zn²⁺ ion. As such, o-phenanthroline is widely used when preparing cell lysates for enrichment of proteins bearing K63-linked polyubiquitin chains which are especially sensitive to DUBs in the JAMM family. Synonym(s): o–phenanthroline Purity: ≥99% Supplied As: 0.5M solution in anhydrous DMSO (100X) Biological Activity: Metalloprotease inhibitor; chelates iron, zinc and other divalent metals. Effective concentration 1-10 mM. Format: DMSO solution Application(s): Useful for inhibition of JAMM-family isopeptidases. Especially valuable for protecting K63-linked polyubiquitin chains in cell lysates. Reference(s): 1. Cooper, E. M., Cutcliffe, C., Kristiansen, T. Z., Pandey, A., Pickart, C. M., and Cohen, R. E. (2009) K63-specific deubiquitination by two JAMM/MPN+ complexes: BRISC-associated Brcc36 and proteasomal Poh1. EMBO J. 28:621-631.2. Sigman, D.S., et al., Nuclease activity of 1,10-phenanthroline-copper in study of protein DNA interactions. Methods Enzymol., 208:414-433 (1991). Warning(s): Protect from moisture. Avoid freeze/thaw cycles. Scientific Category: Ubiquitin Enzymes Product Type: Inhibitors/Activators Data shown is lot-specific. Contact us for specific information on other lots.