FabGennix/StripOBuffer Western Blot Stripping Buffer/FGI-1989/
Overview |
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Product |
StripOBufferTM |
Description | StripOBufferTM is a specially formulated water based buffer that will strip antibodies from antigens in 5-10 minutes at room temperature |
Volume | 100 ML |
Concentration | 10X solution |
Storage | Room temperature for short-term storage |
Applications | 25 mL of StripObuffer can be used on 25-50 mini-blots. |
Save time by re-using blotsPreserve protein and antigen samplesEliminate issues with proper equal protein loadingCompare phosphorylated and non-phosphorylated state of the same proteinon the same blotReconfirm protein expression by re-probing the same blot with different set ofantibodies raised against antibodies from different vendors or same or different epitopesPreform multiple stripping’s while maintaining antigenicityNo heating requiredNo β-mercaptoethanol required – No smellCompatible with Nitrocellulose and PVDF membranesDilute (1:10) the supplied buffer in distilled water prior to use.Rinse the membrane in distilled water first and then in stripping buffer at least once.Multiple rinses with buffer results in clearer bands on autoradiogramsImmerse the membrane completely in 10 mL of stripping buffer for each mini-gel membrane. Gently shake the membrane on a rocker for 5-10 minutes at room temperature.Rinse the membrane with distilled water.Rinse the membrane with TBST or the buffer in which your primary antibody is diluted.Proceed for blocking stage.
