Description: LBP, various species, ELISA kit

Lipopolysacharide (LPS) Binding Protein (LBP) is a type 1 acute phase protein that is constitutively produced by the liver and rapidly upregulated during the acute phase response. LBP plays a central role in the response to LPS. The protein catalyses the monomerization of LPS and its transfer to (s)CD14 and to lipoproteins. In this way LBP has both a role in the activation pathway of LPS: activation of monocytes by LPS leading to release of inflammatory mediators and in the neutralization of LPS i.e. the uptake of LPS by lipoprotein and subsequent clearing. The assay can be used to quantify functional LBP in tissue culture supernatants of cell cultures, plasma and serum samples of several species. In plasma of healthy individuals LBP is present at levels which increase during acute phase responses.

Specifications:

Catalog number Product type Quantity Standard range Detection level Working volume Species Cross reactivity Application Disease Principle Storage and stability Precautions References

HK503

Assays

1 x 96 det.

1.6 to 100 ng/ml

1.6 ng/ml

100 µl/well

N/A

Bovine - Yes, Canine - Yes, Chimpanzee - Yes, Cynomolgus monkey - Yes, Human - Yes, Rabbit - Yes, Rat - Yes, Rhesus monkey - Yes, Sheep - Yes, Swine - Yes

The LBP ELISA kit for a wide variety of species is to be used for the in vitro quantitative determination of LBP in cell culture supernatants, plasma or serum.

Infectious diseases

The LBP ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of 1 plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibo­dies recognizing LBP. Biotinylated LPS will bind to captured LBP. Streptavidin-peroxidase conjugate will bind to the biotinylated LPS. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of citric acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the LBP standards (log). The LBP concentration of samples, which are run concurrently with the human LBP standards, can be determined from the human LBP standard curve.

Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.

1. Gucht, S et al; Porcine Reproductive and respiratory syndrome virus infection increasesCD14 expression and Lipopolysaccharide-Binding Protein in the lungs of pigs. ViralImmunol 2005, 18:116 2. Nikunen, S et al; Association of bovine respiratory disease with clinical status and acutephase proteins in calves. Comp Immunol Microbiol Infect Dis 2007, 3: 143 3. Suojala, L et al; Acute phase response in two consecutive experimentally induced E.coliintramammary infections in dairy cows. Acta Vet Scand 2008, 50: 18 4. Khafipour, E at al; A grain-based subacute ruminal acidosis challenge causestranslocation of lipposaccharide and triggers inflammation. Am Dairy Sci Assoc 2009,92: 1060