Aquaporin 2 phospho S261 Antibody Properties Description Anti-Aquaporin 2 pS261 (Rabbit) Antibody - 612-401-D08 Host Rabbit Target Species Rat Known Cross Reactivity rat Clonality Polyclonal Application ELISA : 1:10,000 Western Blot : 1:1000 Physical State Liquid Shipping Condition Dry Ice Format IgG Label Unconjugated Buffer 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5 Stabilizer 0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol GeneName AQP2 Relevant Links UniProtKB - P34080GeneID - 25386UniProtKB - P34080.1

Aquaporin 2 phospho S261 Antibody Description

Background Aquaporin 2 pS261 antibody recognizes aquaporin 2 (AQP2) which is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans. A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells. Recently, serine-261 has been identified as a novel phosphorylation site on AQP2 and levels of phosphorylated S261 have been shown to decrease with vasopressin treatment suggesting its involvement in vasopressin-dependent AQP2 trafficking. Anti-Aquaporin 2 pS261 antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research. Synonyms ADH water channel, Aquaporin-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct Immunogen Anti-Aquaporin 2 pS261 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser261. Immunogen Type Peptide Post Translational Modification Phosphorylation Storage Condition Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Application Note Anti-Aquaporin 2 pS261 antibody is suitable for use in ELISA, Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 29 kDa AQP2 protein phosphorylated at Ser261 in the appropriate cell lysate or extract. This antibody also recognizes the glycosylated for of aquaporin 2 at approximately 37 kDa in size. Purity/Specifity Anti-Aquaporin 2 pS261 Antibody is directed against rat Aquaporin 2 protein phosphorylated at S261. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Minimal reactivity occurs against non-phosphorylated Aquaporin 2. Reactivity against Aquaporin 2 occurs from rat sources. However, reactivity is also expected against the following species based on 100% sequence homology: canine and bovine, chicken, human, mouse and non-human primate. Disclaimer Note-General This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.