Species: Human Host Species: Sf9 insect cells MW: 142 kDa Genbank #: NM_005813 Tag(s): N-terminal GST-tag a.a: full length Background:PKCν , also known as PKD3, is a members of the protein kinase C (PKC) family of serine/threonine kinases that play critical roles in the regulation of cellular differentiation and proliferation in many cell types. PKCν is composed of 890 amino acid residues and the protein has 77.3% similarity to human PKCµ (PKCmu) and 77. 4% similarity to mouse PKD (1). The PKCν mRNA is ubiquitously expressed in various tissues and the gene is located between markers WI-9798 and D2S177 on chromosome 2p21 region. Description:Recombinant human PKCν (PKCnu), full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system. Synonym(s): EPK2; PRKCN; PKD3; nPKC-NU; PKCν protein kinase c nu Purity: ≥75% Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol. Format: Aqueous buffer solution Storage / Stability:

>6 months at -80°C.

Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Reference(s): Hayashi,A. et al: PKCnu, a new member of the protein kinase C family, composes a fourth subfamily with PKCmu. Biochim Biophys Acta. 1999 May 6;1450(1):99-106. Warning(s): Avoid freeze/thaw cycles Scientific Category: Kinase/Serine-Threonine Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.