product information Background SRK2E (Serine/threonine-protein kinase SRK2E) is an activator of the abscisic acid (ABA) signaling pathway, regulating such ABA responses as: stomato closure, response to drought and plant pathogens.Synonyms: OST1, Open stomata 1 Immunogen KLH-conjugated inique synthetic peptide derived from Arabidopsis thaliana SRK2E sequence UniProt: Q940H6, AT4G33950 Host Rabbit Clonality Polyclonal Clone Purity Affinity purified serum Format Lyophilized in PBS pH 7.5 Quantity 50 µg Reconstitution For reconstitution add 50 µl of sterile water. Storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications western blot (WB), pull-down assay, immunoprecipitation (IP) Related products AS14 2783 | anti-SRK | Ser/Thr-protein kinase SnRK, rabbit antibodiesAS11 1748 | ABA ELISA quantitation kitcollection of antibodies to proteins involved in signal transduction Plant protein extraction buffer Secondary antibodies Additional information

application information
Recommended dilution		1 : 1000 with standard ECL (WB), 10 µg (pull-down assay), 5 µg (IP)
Expected | apparent MW		41 kDa
Confirmed reactivity		Arabidopsis thaliana
Predicted reactivity
Not reactive in		no confirmed exceptions from predicted reactivity are currently known
Additional information		to be added when available
Selected references		to be added when available, antibody released in March 2015

application informationBacterial lysates were separated on 12% SDS-PAGE  and blotted 1h to PVDF using semi-dry or tank transfer. Blots were blocked with 5 % milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 from Agrisera) diluted to 1:50 000 in  for 30 min. at RT with agitation. The blot was washed as above and developed for 3 min with ECL according to the manufacturers instructions. Exposure time was 30 seconds.Courtesy of Dr. Agnieszka Ludwików, UAM, Poznań, Poland

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Protein A agarose beads (40µl) where coated with 10µl (1µg/ul) antibodies and after incubation with amount of extract (10 mg/ml) indicated washed extensively and loaded on gel. In gel kinase assay was performed as described in Fujii, 2007. Autoradiograph shows immunoprecipitated kinase from plant extracts. 1 beads with BSA 20 µl loaded on gel 2 beads with plant extract (WT) 20 µl loaded on gel 3 beeds with plant extract (mutant X) 20 µl loaded on gel 4 beads with BSA 10 µl loaded on gel 5 beads with plant extract (WT) 10 µl loaded on gel 6 beeds with plant extract (mutant X) 10 µl loaded on gel 7 beads with BSA 5 µl loaded on gel 8 beads with plant extract (WT) 5 µl loaded on gel 9 beeds with plant extract (mutant X) 5 µl loaded on gel Courtesy of Dr. Szymon Świeżewski, Institute of Biochemistry and Biophysics, Polish Academy of Science, Warsaw, Poland

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