Description:

This reagent (MAb F7-26) allows the important discrimination between apoptosis and necrosis. In this respect it can be regarded as a unique research tool superior to TUNEL staining. The specificity of the assay is based upon the high sensitivity of DNA to thermal denaturation in condensed chromatin of apoptotic cells. Although there is no change of the original product there have been important improvements in the staining protocol involving a formamide-based DNA-denaturation. This is now more convenient to use, yielding a sharper contrast between apoptotic cells and controls. The increased sensitivity of MAb F7-26 allows omission of a previously recommended Biotin/Streptavidin amplification step, a source of background problems. The APOSTAIN assay is now applicable for cold (4°C) formalin fixed tissue.

Product Specification:

Alternative Name:	Single-stranded DNA
Clone:	F7-26
Host:	Mouse
Isotype:	IgM
Immunogen:	Calf thymus ssDNA.
Species reactivity:	Species independent
Specificity:	Recognizes deoxycytidine of ssDNA of at least 25-30 bases in length in the absence of any reactivity to double-stranded DNA; not species-specific. Specifically detects apoptotic cells; necrotic cells are not recognized.
Applications:	Flow Cytometry, ICC, IHC (FS), IHC (PS)
Recommended Dilutions/Conditions:	Immunohistochemistry (frozen sections, paraffin sections - important: applicable for cold fixation at +4°C - for reference see Manual)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Quantity:	200µl/20 tests; 1ml/100 tests
Formulation:	Liquid. In PBS containing 5% fetal bovine serum (FBS).
Shipping:	Shipped on Dry Ice
Long Term Storage:	-80°C