Agrisera/AKING1 | SNF1-related protein kinase regulatory subunit gamma 1/AS09 613/
| | product information | | Background | | AKING1 is a member of SnRK1/SNF1/AMPK family in plants. It is a regulatory subunit of the putative trimeric SNF1-related protein kinase (SnRK) comples which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants. | | Immunogen | | KLH-conjugated peptide derived from Arabidopsis thaliana AKING1 Q8LBB2, At3g48530 | | Host | | Rabbit | | Clonality | | Polyclonal | | Clone | | | | Purity | | Affinity purified serum in PBS pH 7.4. | | Format | | Lyophilized | | Quantity | | 50 µg | | Reconstitution | | For reconstitution add 50 µl of sterile water. | | Storage | | store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. | | Tested applications | | western blot (WB) | | Related products | | AS09 460 | Anti-AKINB1 | SNF1-related protein kinase regulatory subunit beta-1, rabbit antibodyAS09 462 | Anti-AKINB2 | SNF1-related protein kinase regulatory subunit beta-2, rabbit antibodyAS15 2859 | Anti-AKIN ß3 | SNF1-related protein kinase regulatory subunit beta-3, rabbit antibodyAS09 463 | Anti-AKIN beta gamma, rabbit antibodyAS10 919 | Anti-AKIN10 SNF1-related protein kinase catalytic subunit alpha KIN10, rabbit antibodyAS10 920 | Anti-AKIN11 | SNF1-related protein kinase catalytic subunit alpha KIN11, rabbit antiboddycollection of antibodies to proteins involved in signal transduction Plant protein extraction buffer Secondary antibodies | | Additional information | | | |
| application information |
| Recommended dilution | | 1: 2000 (WB) |
| Expected | apparent MW | | 48 kDa |
| Confirmed reactivity | | Arabidopsis thaliana |
| Predicted reactivity | | Arabidopsis thaliana |
| Not reactive in | | Chlamydomonas reinhardtii, Vitis vinifera |
| Additional information | | |
| Selected references | | Emanuelle et al. (2015). SnRK1 from Arabidopsis thaliana is an atypical AMPK. Plant J. 2015 Mar 3. doi: 10.1111/tpj.12813.Ramon et al. (2013). The hybrid Four-CBS-Domain KINβγ-subunit functions as the canonical γsubunit of the plant energy sensor SnRK1. Plant j. April 1. |
application example
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10 ug of Arabidopsis thaliana Columbia WT and KIN gamma KO seedlings were grinded in MOPS loading buffer and heated for 5 min at 95°C. Samples were separated on 10 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody, diluted to 1:4 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 15 seconds.
Courtesy of Dr. Matthew Ramon
