Species: Human Host Species: FreeStyle 293-F cells MW: 37 kDa Genbank #: NM_004079.3 Tag(s): C-terminal His-tag a.a: CD33 1–16, CathepsinS 17–331 Description:Human cathepsin S (GenBank Accession No. NM_004079.3), CD33 signal peptide(a.a. 1-16) + cathepsin S (a.a. 17-331), with C-terminal His-tag, MW=37 kDa, expressed in FreeStyle 293-F cells. UniProt P25774 Synonym(s): CTSS Specific Activity: ≥ 700 pmole/min/µg Assay Conditions: The enzymes were diluted in 25 mM MES buffer pH5, 0.1 M NaCl, 5 mM DTT, and activated by low pH at room temperature for 2 hours. After addition of 10 μM substrate (Z-L-R-AMC) a kinetic reaction was followed for 30 minutes at room temperature and the fluorescence intensity was measured at exc360/em460. Formulation: 45 mM Tris7HCl, pH 8.0,124 mM NaCl, 2.4 mM KCl, 225 mMimidazole, 3 mM DTT, and 10% glycerol Format: Aqueous buffer solution Storage / Stability:

>6 months at -80°C.

Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Reference(s): 1. Seo H.R. et al., Int. J. Cancer 124 (8),1794-1801 (2009).2. Lutzner N. and Kalbacher H. J. Biol.Chem. 283 (52), 36185-36194 (2008). Warning(s): Avoid freeze/thaw cycles Scientific Category: Protease Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.