A potent, irreversible and specific inhibitor of deubiquitinating enzymes (DUBs) that is prepared by chemical synthesis. It is N-terminally tagged with an HA-tag (YPYDVPDYA), which allows for sensitive identification or purification of DUBs since it is specifically recognized by anti-HA antibodies. The HA tag is separated from the Ub N-terminus by two aminohexanoic acid (Ahx) linkers for efficient recognition of the HA tag.

Additional Information

Product Name: Also Known As: Catalog No.: Size: Molecular Weight: Species: Source: Stock: Concentration: Quality Assurance: Storage: PDF Data Sheet: NCBI RefSeq: Image(s): Shipping Method: References:

HA-Ub-VME

N/A

M1010

25 µg

9913 Da by MS

Human

Synthetic

Product in lyophilized powder form.

N/A

≥95% by RP-HPLC

Powder at −20°C; solution at −80°C. Avoid multiple freeze/thaw cycles.

PDF Datasheet， MSDS

N/A

(Click image to enlarge)

Coomassie-stained SDS-PAGELane 1: HA-Ub-VMELane 2: UbiquitinLane 3: Molecular weight markers

Room temperature shipping

1. de Jong et al., (2012) ChemBioChem 13, 2251.2. Borodovsky et al., (2001) EMBO J. 20, 5187. 3. Borodovsky et al., (2002) Chemistry and Biology 9, 1149. 4. El Oualid et al., (2010) Angew. Chem. Int. Ed. 49, 10149.

Details

A potent, irreversible and specific inhibitor of deubiquitinating enzymes (DUBs) that is prepared by chemical synthesis. It is N-terminally tagged with an HA-tag (YPYDVPDYA), which allows for sensitive identification or purification of DUBs since it is specifically recognized by anti-HA antibodies. The HA tag is separated from the Ub N-terminus by two aminohexanoic acid (Ahx) linkers for efficient recognition of the HA tag.Sample Preparation:Dissolve the powder in DMSO (e.g. add 1.25 μl DMSO to 25 μg powder to get 20 mg/ml or 2.25 mM DMSO stock) and add this DMSO stock to the required buffer (please note the order of addition). Typical reaction concentrations vary from 0.2 - 2 mM.Images:(Click image to enlarge) Coomassie-stained SDS-PAGELane 1: HA-Ub-VMELane 2: UbiquitinLane 3: Molecular weight markers