Description:

Short Description: In this assay the Alpha 2-Macroglobulin present in serum sample reacts with the anti-A2M. antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound serum proteins by washing, anti-A2M. antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound serum A2M. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Alpha 2-Macroglobulin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Alpha 2-Macroglobulin in the test sample. The quantity of Alpha 2-Macroglobulin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for serum dilution.

Additional Information:

Name Related Product Names Alias Symbols Protein Name Description of Target Swissprot ID Species Reactivity Assay Info Sensitivity Kit Range Homology NCBI Acc Number Standard Range Sample Type Product Format Specimen/Volumes Swiss Prot Number Applications Reconstitution and Storage Reactivity Kit Size Kit Principle Kit Component Kit Duration Storage Lead Time Intended Use Additional Information ::

Rat Alpha 2-Macroglobulin ELISA Kit

ALPHA 2M. CPAMD5; Rat Alpha 2-Macroglobulin ELISA Kit; Alpha-2-M; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 CPAMD5Rat Alpha 2-Macroglobulin A2M

A2M, Alpha-2-macroglobulin, CPAMD5

Pregnancy zone protein

Is able to inhibit all four classes of proteinases by a unique trapping mechanism. This protein has a peptide stretch, called the bait region which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region,

Q61838

Rat

Assay Methodology: Quantitative Sandwich ELISA

2.330 ng/ml

6.25 ng/ml - 400 ng/ml

Rat

NP_000005.2

Refer To Manual

Serum, plasma and other biological fluids

In Solution

Refer To Manual

P01023

ELISA

1. DILUENTThe 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.2. WASH SOLUTIONThe 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.3. ENZYME-ANTIBODY CONJUGATEUndiluted horseradish peroxidase anti-A2M conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.4. CHROMOGEN-SUBSTRATE SOLUTIONThe Substrate Solution should be stored at 4-8C and is stable until the expiration date.5. STOP SOLUTIONThe Stop Solution should be stored at 4-8C and is stable until the expiration date.6. ANTI-RAT ALPHA 2-MACROGLOBULIN ELISA MICRO PLATEAnti-Rat A2M coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.7. RAT ALPHA 2-MACROGLOBULIN CALIBRATORThe lyophilized Rat Alpha 2-Macroglobulin calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.

Rat

96 well

Genway Biotech Alpha-2 Macroglobulin ELISA Kit (Rat) (GWB-98EFFB) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Pregnancy zone protein has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Pregnancy zone protein - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pregnancy zone protein captured in the well.

ComponentAmount Anti-Rat Alpha-2 Macroglobulin Microplate 96 Wells (12 x 8 Well Strips) 20X Diluent Concentrate 50 mL 20X Wash Solution 50 mL 100X Enzyme-Antibody Conjugate 150 uL Chromogen-Substrate Solution 12 mL Stop Solution 12 mL Rat Alpha-2 Macroglobulin Calibrator 1

80 Minutes

Refer to kit instructions for component storage

Domestic: within 1 week deliveryInternational: 1 week

Research Use Only

Intended Use: The Alpha 2-Macroglobulin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Alpha 2-Macroglobulin in biological samples of rats.

Reagents: 1. DILUENT CONCENTRATE (Running Buffer)One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution.3. ENZYME-ANTIBODY CONJUGATE 100XOne vial containing 150 ul of affinity purified anti-Rat Alpha 2-Macroglobulin antibody conjugated with horseradish peroxidase in a stabilizing buffer.4. CHROMOGEN-SUBSTRATE SOLUTIONOne vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.5. STOP SOLUTIONOne vial containing 12 ml 0.3 M sulfuric acid.WARNING: Avoid contact with skin.6. ANTI-RAT ALPHA 2-MACROGLOBULIN ELISA MICRO PLATETwelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Rat A2M.7. RAT ALPHA 2-MACROGLOBULIN CALIBRATOROne vial containing a lyophilized Rat Alpha 2-Macroglobulin calibrator.