Background
Eotaxin-3 is a CC chemokine that signals through the CCR3 receptor. It is produced by endothelial cells stimulated with IL-4 or IL-13. Eotaxin-3 selectively targets cells expressing CCR3, including eosinophils, basophils, T cells and monocytes. Eotaxin-3 has similar activity to Eotaxin and Eotaxin-2, but the three Eotaxins share only a low degree of sequence homology. Recombinant human Eotaxin-3 is an 8.4 kDa protein containing 71 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.
Specifications
Additional Names
CCL26Preparation
Biological activity was determined by its ability to chemoattract human CCR3/HEK 293 cells. The maximum activity was achieved at 2ug/ml.Location
TRGSDISKTC CFQYSHKPLP WTWVRSYEFT SNSCSQRAVI FTTKRGKKVC THPRKKWVQK YISLLKTPKQ LSource
E. coliReactivity
n/aTested Application
n/aStorage
The lyophilized Eotaxin-3 recombinant protein is stable for at least 2 years from date of receipt at -20ËšC. Reconstituted Eotaxin-3 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20ËšC. As with any protein, exposing Eotaxin-3 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.Species Reactivity
n/aPurity
Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
