Description:

BG-Maleimide is a thiol-reactive building block for the one-step synthesis of SNAP-tag^® substrates from thiol-containing precursors such as thiol-modified oligonucleotides. This building block allows you to make custom SNAP-tag substrates for labeling SNAP-tag fusion proteins for a wide range of applications such as the coupling of proteins and solid surfaces such as DNA chips (1). The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O⁶-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described in this document.

Figure 1: Structure of BG-Maleimide (MW 489.5 g/mol)

Notes:

Dry BG-Maleimide should be stored at -20°C.The BG-Maleimide building block should be dissolved in anhydrous DMF. Choose a solvent volume that will be compatible with the final use. Unused building block dissolved in DMF should be stored at -20°C.